WOMEN IN CANCER THEMATIC REVIEW: Ovarian cancer–peritoneal cell interactions promote extracellular matrix processing

Ovarian cancer has a distinct tendency for metastasising via shedding of cancerous cells into the peritoneal cavity and implanting onto the peritoneum that lines the pelvic organs. Once ovarian cancer cells adhere to the peritoneal cells, they migrate through the peritoneal layer and invade the local organs. Alterations in the extracellular environment are critical for tumour initiation, progression and intra-peritoneal dissemination. To increase our understanding of the molecular mechanisms involved in ovarian cancer metastasis and to identify novel therapeutic targets, we recently studied the interaction of ovarian cancer and peritoneal cells using a proteomic approach. We identified several extracellular matrix (ECM) proteins including, fibronectin, TGFBI, periostin, annexin A2 and PAI-1 that were processed as a result of the ovarian cancer–peritoneal cell interaction. This review focuses on the functional role of these proteins in ovarian cancer metastasis. Our findings together with published literature support the notion that ECM processing via the plasminogen–plasmin pathway promotes the colonisation and attachment of ovarian cancer cells to the peritoneum and actively contributes to the early steps of ovarian cancer metastasis.

Pengaruh Insuflasi terhadap Jumlah Sel Mast Peritoneum Tikus

Peritoneal insufflation is swelling process to perform laparoscopic surgery, the choice of gas for insufflation in laparoscopic surgery, namely CO2. because it is not flammable bleak N2O, so it can be used safely for diathermy in mice. This study aims to determine whether there is influence insufflation in an increased number of mast cells in rats (Rattus norvegicus). This study is pure experimental research with posttest-only control group design, which consists of the control and treatment of pressure 8 mmHg and 10 mmHg, with the results obtained p0 control = 0.52 p1 8 mmHg = 7.84 p2 10 mmHg = 11.94. ANOVA test showed that the increase in the number of mast cells was significantly occurred in the group with a pressure of 10 mmHg as compared to the pressure of 8 mmHg. Based on research carried out can be concluded that the higher the CO2 gas in the insufflation pressure significantly increases the number of mast cells.Keywords: insuflation, peritoneal, cell Mast Abstrak: Insuflasi adalah proses penggembugan peritoneum untuk melakukan pembedahan laparoskopi, pilihan gas untuk insuflasi pada bedah laparoskopi yaitu CO2. karena tidak mudah terbakar seprti N2O, sehingga dapat digunakan secara aman untuk diatermi pada tikus. Penelitian ini bertujuan untuk mengetahui apakah ada pengaruh insuflasi dalam peningkatan jumlah sel mast pada tikus putih (Rattus norvegicus). penelitian ini merupakan penelitian experimental murni dengan posttest-only with control group design, yang terdiri dari kontrol dan perlakuan dengan tekanan 8 mmHg dan 10 mmHg, dengan hasil yang didapatkan p0 kontrol = 0,52 p1 8 mmHg = 7,84 p2 10 mmHg=11,94. Uji anova menunjukan bahwa peningkatan jumlah sel mast secara bermakna terjadi pada kelompok dengan tekanan 10 mmHg dibandingkan dengan tekanan 8 mmHg. Berdasarkan penelitian yang dilakukan dapat diambil kesimpulan bahwa semakin tinggi tekanan gas CO2 pada insuflasi secara bermakna dapat meningkatkan jumlah sel mast. Kata-kata Kunci :Insuflasi, sel mast, peritoneum