Molecular analysis of deletion mutations at the HPRT locus in CHO cells using the polymerase chain reaction

Author(s):  
Z.D. Xu ◽  
Y. Yu ◽  
B.J.F. Rossiter ◽  
R.A. Gibbs ◽  
C.T. Caskey ◽  
...  
2019 ◽  
Vol 29 (2) ◽  
pp. 116-120
Author(s):  
M. C. F. Silva‐Malta ◽  
C. C. S. Santos ◽  
P. C. Gonçalves ◽  
L. C. Schmidt ◽  
M. L. Martins

Medicine ◽  
2015 ◽  
Vol 94 (47) ◽  
pp. e2067 ◽  
Author(s):  
Francisco Artur Forte Oliveira ◽  
Clarissa Pessoa Fernandes Forte ◽  
Paulo Goberlânio de Barros Silva ◽  
Camile B. Lopes ◽  
Raquel Carvalho Montenegro ◽  
...  

Author(s):  
Rosnizar Rosnizar ◽  
Kartini Eriani

This study aimed to analyze MSP-1 gene in mice erythrocyte infected with Plasmodium berghei using polymerase chain reaction (PCR). The result showed the existence of 462 bp DNA band which was assumed to encode MSP-1 protein with molecular weight of 19 kDa that can only be found in erythrocyte infected with Plasmodium berghei. BLASTn analysis showed that PbMSP-1 obtained in this study have 100% similar identity with mRNA of PbMSP-1 partial sequence (462 bp), 93% similarity with PbMSP-1 complete sequence (5750 bp and 5376 bp), and 87% similarity with PbMSP-1 incomplete sequence (333 bp).


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