Altered cytokine genes expression by ConA-activated spleen cells from mice infected by lymphocytic choriomeningitis virus

1993 ◽  
Vol 35 (3) ◽  
pp. 247-253 ◽  
Author(s):  
Jean-Hervé Colle ◽  
Marie-Françoise Saron ◽  
Paolo Truffa-Bachi
Keyword(s):  
Lymphocytic Choriomeningitis ◽  
Lymphocytic Choriomeningitis Virus ◽  
Spleen Cells ◽  
Cytokine Genes ◽  
Genes Expression

scholarly journals Selection of genetic variants of lymphocytic choriomeningitis virus in spleens of persistently infected mice. Role in suppression of cytotoxic T lymphocyte response and viral persistence.

1984 ◽  
Vol 160 (2) ◽  
pp. 521-540 ◽  
Author(s):  
R Ahmed ◽  
A Salmi ◽  
L D Butler ◽  
J M Chiller ◽  
M B Oldstone
Keyword(s):  
T Lymphocyte ◽  
Genetic Variants ◽  
Cytotoxic T Lymphocyte ◽  
Lymphocytic Choriomeningitis ◽  
Lymphocytic Choriomeningitis Virus ◽  
Spleen Cells ◽  
Ctl Response ◽  
Immunocompetent Mice ◽  
Lcmv Infection ◽  
Ctl Responses

We studied the mechanism of lymphocytic choriomeningitis virus (LCMV) persistence and the suppression of cytotoxic T lymphocyte (CTL) responses in BALB/c WEHI mice infected at birth with LCMV Armstrong strain. Using adoptive transfer experiments we found that spleen cells from persistently infected (carrier) mice actively suppressed the expected LCMV-specific CTL response of spleen cells from normal adult mice. The suppression was specific for the CTL response and LCMV -specific antibody responses were not affected. Associated with the specific CTL suppression was the establishment of persistent LCMV infection. The transfer of spleen or lymph node cells containing LCMV -specific CTL resulted in virus clearance and prevented establishment of the carrier state. The suppression of LCMV -specific CTL responses by carrier spleen cells is not mediated by a suppressor cell, but is due to the presence of genetic variants of LCMV in spleens of carrier mice. Such virus variants selectively suppress LCMV-specific CTL responses and cause persistent infections in immunocompetent mice. In striking contrast, wild-type LCMV Armstrong, from which these variants were generated, induces a potent CTL response in immunocompetent mice and the LCMV infection is rapidly cleared. Our results show that LCMV variants that emerge during infection in vivo play a crucial role in the suppression of virus-specific CTL responses and in the maintenance of virus persistence.

scholarly journals Effector T lymphocytes in lymphocytic choriomeningitis virus-infected mice. Cytolytic activity of Lyt-23 spleen cells in vitro does not correlate with elimination of infectious virus from spleens.

1981 ◽  
Vol 153 (4) ◽  
pp. 992-997 ◽  
Author(s):  
M Varho ◽  
F Lehmann Grube ◽  
M M Simon
Keyword(s):  
T Lymphocytes ◽  
Infectious Virus ◽  
Lymphocytic Choriomeningitis ◽  
Cytolytic Activity ◽  
Lymphocytic Choriomeningitis Virus ◽  
Target Cells ◽  
Spleen Cells ◽  
Marginal Effects ◽  
Infected Cells

The T lymphocytes from mice recovering from infection with lymphocytic choriomeningitis virus were selected for subclasses by treatment with anti-Lyt antisera and complement. Lyt-23 cells and mixtures of lyt-1 and Lyt-23 cells caused up to one-half the destruction of cultivated target cells as compared with untreated T lymphocytes; Lyt-1 cells alone were not cytotoxic. Selected and unselected spleen T cells were also inoculated intravenously into previously infected mice. Whereas unselected cells reduced infectious virus in the spleens of the recipients approximately 100-fold, only marginal effects, which were not preferentially associated with one particular subclass, were seen with selected LYT-23 or Lyt-1 lymphocytes or a mixture of both. Apparently the Lyt-23 cells, shown to by cytolytic for infected cells in vitro, did not cause elimination of a measurable quantity of the virus from mice.

Antiviral Effect of Interferon Lambda Against Lymphocytic Choriomeningitis Virus

2015 ◽  
Vol 35 (7) ◽  
pp. 540-553 ◽  
Author(s):  
Lubomira Lukacikova ◽  
Ingrid Oveckova ◽  
Tatiana Betakova ◽  
Katarina Laposova ◽  
Katarina Polcicova ◽  
...  
Keyword(s):  
Antiviral Effect ◽  
Lymphocytic Choriomeningitis ◽  
Lymphocytic Choriomeningitis Virus ◽  
Interferon Lambda

scholarly journals The Nucleoprotein Is Required for Lymphocytic Choriomeningitis Virus-Based Vaccine Vector Immunogenicity

2015 ◽  
Vol 89 (22) ◽  
pp. 11734-11738 ◽  
Author(s):  
Stephanie Darbre ◽  
Susan Johnson ◽  
Sandra Kallert ◽  
Paul-Henri Lambert ◽  
Claire-Anne Siegrist ◽  
...  
Keyword(s):  
Gene Expression ◽  
T Cell ◽  
T Cell Responses ◽  
Lymphocytic Choriomeningitis ◽  
Lymphocytic Choriomeningitis Virus ◽  
Viral Transcription ◽  
Genome Replication ◽  
Recombinant Glycoprotein ◽  
Cell Responses ◽  
Deficient Vector

Recombinant glycoprotein-deficient lymphocytic choriomeningitis virus-based vaccine vectors (rLCMV/ΔGP) are potent CD8+T cell inducers. To investigate the underlying molecular requirements, we generated a nucleoprotein-deficient vector counterpart (rLCMV/ΔNP). NP but not GP is a minimaltrans-acting factor for viral transcription and genome replication. We found that, unlike rLCMV/ΔGP, rLCMV/ΔNP failed to elicit detectable CD8+T cell responses unless NP wastranscomplemented in a transgenic host. Hence, NP-dependent intracellular gene expression is essential for LCMV vector immunogenicity.

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