Intratypic differentiation of poliovirus strains by enzyme-linked immunosorbent assay (ELISA): poliovirus type 2 and poliovirus type 3

1987 ◽  
Vol 18 (1) ◽  
pp. 25-36 ◽  
Author(s):  
Graciela Glikmann ◽  
Maria Pedersen ◽  
Inger Petersen
Keyword(s):  
Immunosorbent Assay ◽  
Enzyme Linked Immunosorbent Assay ◽  
Poliovirus Type ◽  
Type 3

scholarly journals Case of Poliomyelitis Caused by Significantly Diverged Derivative of the Poliovirus Type 3 Vaccine Sabin Strain Circulating in the Orphanage

Viruses ◽  
2020 ◽  
Vol 12 (9) ◽  
pp. 970
Author(s):  
Ekaterina A. Korotkova ◽  
Maria A. Prostova ◽  
Anatoly P. Gmyl ◽  
Liubov I. Kozlovskaya ◽  
Tatiana P. Eremeeva ◽  
...  
Keyword(s):  
Genome Sequencing ◽  
Mucosal Immunity ◽  
Neutralizing Antibodies ◽  
Oral Poliovirus Vaccine ◽  
Paralytic Poliomyelitis ◽  
Epidemiological Investigation ◽  
Poliovirus Type ◽  
Complete Rejection ◽  
Type 3

Significantly divergent polioviruses (VDPV) derived from the oral poliovirus vaccine (OPV) from Sabin strains, like wild polioviruses, are capable of prolonged transmission and neuropathology. This is mainly shown for VDPV type 2. Here we describe a molecular-epidemiological investigation of a case of VDPV type 3 circulation leading to paralytic poliomyelitis in a child in an orphanage, where OPV has not been used. Samples of feces and blood serum from the patient and 52 contacts from the same orphanage were collected twice and investigated. The complete genome sequencing was performed for five polioviruses isolated from the patient and three contact children. The level of divergence of the genomes of the isolates corresponded to approximately 9–10 months of evolution. The presence of 61 common substitutions in all isolates indicated a common intermediate progenitor. The possibility of VDPV3 transmission from the excretor to susceptible recipients (unvaccinated against polio or vaccinated with inactivated poliovirus vaccine, IPV) with subsequent circulation in a closed children’s group was demonstrated. The study of the blood sera of orphanage residents at least twice vaccinated with IPV revealed the absence of neutralizing antibodies against at least two poliovirus serotypes in almost 20% of children. Therefore, a complete rejection of OPV vaccination can lead to a critical decrease in collective immunity level. The development of new poliovirus vaccines that create mucosal immunity for the adequate replacement of OPV from Sabin strains is necessary.

A poliovirus type-specific IgM antibody-capture enzyme-linked immunosorbent assay for the rapid diagnosis of poliomyelitis

1994 ◽  
Vol 2 (2) ◽  
pp. 113-126 ◽  
Author(s):  
Ria Nibbeling ◽  
Johan H.J. Reimerink ◽  
Mubina Agboatwala ◽  
Tary Naquib ◽  
Albert Ras ◽  
...  
Keyword(s):  
Immunosorbent Assay ◽  
Rapid Diagnosis ◽  
Enzyme Linked Immunosorbent Assay ◽  
Poliovirus Type ◽  
Igm Antibody ◽  
Antibody Capture

scholarly journals Postabsorptive hyperglucagonemia in patients with type 2 diabetes mellitus analyzed with a novel enzyme‐linked immunosorbent assay

2015 ◽  
Vol 7 (3) ◽  
pp. 324-331 ◽  
Author(s):  
Toshihiro Matsuo ◽  
Jun‐ichiro Miyagawa ◽  
Yoshiki Kusunoki ◽  
Masayuki Miuchi ◽  
Takashi Ikawa ◽  
...  
Keyword(s):  
Diabetes Mellitus ◽  
Type 2 Diabetes ◽  
Type 2 Diabetes Mellitus ◽  
Immunosorbent Assay ◽  
Enzyme Linked Immunosorbent Assay

Enzyme-linked immunosorbent assay for circulating human resistin: resistin concentrations in normal subjects and patients with type 2 diabetes

2004 ◽  
Vol 339 (1-2) ◽  
pp. 57-63 ◽  
Author(s):  
Aya Fujinami ◽  
Hiroshi Obayashi ◽  
Kiyoe Ohta ◽  
Toshiko Ichimura ◽  
Masataka Nishimura ◽  
...  
Keyword(s):  
Type 2 Diabetes ◽  
Immunosorbent Assay ◽  
Normal Subjects ◽  
Enzyme Linked Immunosorbent Assay ◽  
Human Resistin
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