Single step purification of biologically active recombinant rat basic fibroblast growth factor by immobilized metal affinity chromatography

1995 ◽  
Vol 1250 (1) ◽  
pp. 29-34 ◽  
Author(s):  
Michael Kroiher ◽  
Simona Raffioni ◽  
Robert E. Steele
Keyword(s):  
Growth Factor ◽  
Affinity Chromatography ◽  
Fibroblast Growth Factor ◽  
Basic Fibroblast Growth Factor ◽  
Single Step ◽  
Biologically Active ◽  
Immobilized Metal Affinity Chromatography ◽  
Fibroblast Growth ◽  
Metal Affinity ◽  
Single Step Purification

scholarly journals Plant-Produced Basic Fibroblast Growth Factor (bFGF) Promotes Cell Proliferation and Collagen Production

2020 ◽  
Vol 07 (04) ◽  
pp. e150-e157
Author(s):  
Kaewta Rattanapisit ◽  
Angkana Jantimaporn ◽  
Pornjira Kaewpungsup ◽  
Balamurugan Shanmugaraj ◽  
Prasit Pavasant ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
Growth Factor ◽  
Fibroblast Growth Factor ◽  
Basic Fibroblast Growth Factor ◽  
Signal Peptide ◽  
Dermal Fibroblast ◽  
Biologically Active ◽  
Vector System ◽  
Fibroblast Growth ◽  
Collagen Production

AbstractHuman fibroblast growth factor regulates a broad spectrum of biological functions, including cell proliferation and tissue differentiation, and has a wider application in tissue engineering. Here, we described the production of human basic fibroblast growth factor in plants by using a geminiviral vector system. In this study, we transiently expressed basic fibroblast growth factor containing a C-terminus 8X-Histidine with and without a barley alpha amylase signal peptide in Nicotiana benthamiana. The expression level of basic fibroblast growth factor without the signal peptide was found to be higher than the basic fibroblast growth factor with the signal peptide. Further, the recombinant basic fibroblast growth factor was purified from the plant crude extract by two-step purification viz., ammonium sulfate precipitation and Ni-affinity chromatography. Our results demonstrated that the purified plant-produced basic fibroblast growth factor was biologically active and promotes the proliferation of human periodontal ligament stem cells and human follicle dermal papilla cells in vitro. Moreover, the plant-produced basic fibroblast growth factor also induced collagen production in human dermal fibroblast cells. Our results suggest the potential use of plant-produced basic fibroblast growth factor as an antiaging and hair growth-promoting agent in the cosmetic industry.

Expression of Biologically Active Basic Fibroblast Growth Factor by Genetically Modified Rat Primary Skin Fibroblasts

2002 ◽  
Vol 64 (2) ◽  
pp. 503-513 ◽  
Author(s):  
Jasodhara Ray ◽  
Joanna Hogg ◽  
Andreas S. Beutler ◽  
Hideichi Takayama ◽  
Andrew Baird ◽  
...  
Keyword(s):  
Growth Factor ◽  
Fibroblast Growth Factor ◽  
Basic Fibroblast Growth Factor ◽  
Genetically Modified ◽  
Skin Fibroblasts ◽  
Biologically Active ◽  
Fibroblast Growth

Properly Oriented Heparin−Decasaccharide-Induced Dimers Are the Biologically Active Form of Basic Fibroblast Growth Factor

Biochemistry ◽  
1997 ◽  
Vol 36 (25) ◽  
pp. 7936-7936
Author(s):  
Franklin J. Moy ◽  
Michal Safran ◽  
Andrew P. Seddon ◽  
Doug Kitchen ◽  
Peter Böhlen ◽  
...  
Keyword(s):  
Growth Factor ◽  
Fibroblast Growth Factor ◽  
Basic Fibroblast Growth Factor ◽  
Active Form ◽  
Biologically Active ◽  
Fibroblast Growth
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