scholarly journals Hydrogen peroxide activation of cytosolic phospholipase A2 in vascular smooth muscle cells

1995 ◽  
Vol 1265 (1) ◽  
pp. 67-72 ◽  
Author(s):  
G.N. Rao ◽  
M.S. Runge ◽  
R.W. Alexander
Keyword(s):  
Hydrogen Peroxide ◽  
Smooth Muscle ◽  
Vascular Smooth Muscle ◽  
Smooth Muscle Cells ◽  
Phospholipase A2 ◽  
Vascular Smooth Muscle Cells ◽  
Muscle Cells ◽  
Cytosolic Phospholipase A2 ◽  
Cytosolic Phospholipase ◽  
Peroxide Activation

scholarly journals Orai1 and Ca2+-independent phospholipase A2 are required for store-operated Icat-SOC current, Ca2+ entry, and proliferation of primary vascular smooth muscle cells

2012 ◽  
Vol 302 (5) ◽  
pp. C748-C756 ◽  
Author(s):  
Bo Yang ◽  
Tomasz Gwozdz ◽  
Joanna Dutko-Gwozdz ◽  
Victoria M. Bolotina
Keyword(s):  
Smooth Muscle ◽  
Vascular Smooth Muscle ◽  
Smooth Muscle Cells ◽  
Dna Synthesis ◽  
Phospholipase A2 ◽  
Cell Lines ◽  
Vascular Smooth Muscle Cells ◽  
Muscle Cells ◽  
Multiple Functions

Store-operated Ca2+ entry (SOCE) is important for multiple functions of vascular smooth muscle cells (SMC), which, depending of their phenotype, can resemble excitable and nonexcitable cells. Similar to nonexcitable cells, Orai1 was found to mediate Ca2+-selective (CRAC-like) current and SOCE in dedifferentiated cultured SMC and smooth muscle-derived cell lines. However, the role of Orai1 in cation-selective store-operated channels (cat-SOC), which are responsible for SOCE in primary SMC, remains unclear. Here we focus on primary SMC, and assess the role of Orai1 and Ca2+-independent phospholipase A2 (iPLA2β, or PLA2G6) in activation of cat-SOC current ( Icat-SOC), SOCE, and SMC proliferation. Using molecular, electrophysiological, imaging, and functional approaches, we demonstrate that molecular knockdown of either Orai1 or iPLA2β leads to similar inhibition of the whole cell cat-SOC current and SOCE in primary aortic SMC and results in significant reduction in DNA synthesis and impairment of SMC proliferation. This is the first demonstration that Orai1 and iPLA2β are equally important for cat-SOC, SOCE, and proliferation of primary aortic SMC.

scholarly journals Probucol inhibits hydrogen peroxide to induce apoptosis of vascular smooth muscle cells

2013 ◽  
Vol 7 (4) ◽  
pp. 1185-1190 ◽  
Author(s):  
LIN SHENG ◽  
BO JIAO ◽  
LIJUAN SHAO ◽  
SHAOJIE BI ◽  
CHAO CHENG ◽  
...  
Keyword(s):  
Hydrogen Peroxide ◽  
Smooth Muscle ◽  
Vascular Smooth Muscle ◽  
Smooth Muscle Cells ◽  
Vascular Smooth Muscle Cells ◽  
Muscle Cells
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