Human recombinant LKM (P150 DB1) antigen is a sensitive and specific diagnostic reagent for differential diagnosis of chronic hepatitis

Raised levels of platelet-associated IgG have been described in many pathological conditions such as ITP, LES, rheumatoid arthritis, lymphoma, drug thrombocytopenia. We measured surface-bound platelet IgG in 60 patients suffering from chronic hepatitis. In all cases diagnosis was confirmed by needle biopsy of the liver and by pertinent laboratory data; the technique used was that described by Dixon et al.(1975). In chronic active hepatitis (40 cases) we found increased amounts of platelet-associated IgG with values ranging from 110 to 480 ng/107 platelets (mean 195 ng; < 0.O01). In persistent hepatitis (40 cases) surface-bound platelet IgG were always less than 70 ng/107 platelets. A significant inverse correlation between increased amount of platelet IgG and the platelet count was not found. This test can be of considerable help in the differential diagnosis of chronic hepatitis as well in the follow up of these patients for prognostic and therapeutic decisions.

Download Full-text

Multivariate analysis of an enzymic profile for the differential diagnosis of viral hepatitis.

Clinical Chemistry ◽

10.1093/clinchem/26.13.1897 ◽

1980 ◽

Vol 26 (13) ◽

pp. 1897-1899 ◽

Cited By ~ 14

Author(s):

G Plomteux

Keyword(s):

Differential Diagnosis ◽

Chronic Hepatitis ◽

Viral Hepatitis ◽

Glutamate Dehydrogenase ◽

Alanine Aminotransferase ◽

Liver Diseases ◽

Acute Viral Hepatitis ◽

Principal Component ◽

Liver Function Tests ◽

Alanine Aminotransferase Activity

Abstract Differential diagnosis of acute viral hepatitis, persistent chronic hepatitis, aggressive chronic hepatitis, and post-necrotic cirrhosis can reasonably be achieved on the basis of three well-known liver-function tests: aspartate aminotransferase, alanine aminotransferase, and glutamate dehydrogenase. With use of principal-component analysis, these four liver diseases can be characterized by two criteria: a “cytolytic” criterion, correlated particularly with a membrane-permeability test—namely, alanine aminotransferase activity—and a “mitochondrial damage” criterion, which is associated with above-normal ornithine carbamyltransferase and glutamate dehydrogenase activities.

Download Full-text

Histological Differential Diagnosis of Chronic Hepatitis

Chronic Hepatitis ◽

10.1159/000399257 ◽

2015 ◽

pp. 10-17 ◽

Cited By ~ 2

Author(s):

V. J. Desmet

Keyword(s):

Differential Diagnosis ◽

Chronic Hepatitis

Download Full-text

Diagnostic and economic efficiency of schemes for differential diagnosis of chronic hepatitis and liver cirrhosis, based on the determination of interleukin-13, alpha-1-defensin and enzymatic activities in serum

Immunopathology Allergology Infectology ◽

10.14427/jipai.2020.4.84 ◽

2020 ◽

pp. 84-92

Author(s):

H Yupatov ◽

V Pryshchepenko

Keyword(s):

Differential Diagnosis ◽

Liver Cirrhosis ◽

Chronic Hepatitis ◽

Liver Biopsy ◽

Blood Serum ◽

Economic Effect ◽

Enzymatic Activities ◽

Diagnostic Efficiency ◽

Interleukin 13

The search for non-invasive criteria for the differential diagnosis of chronic hepatitis and liver cirrhosis is one of the urgent tasks of hepatology. Purpose: to evaluate the diagnostic and economic efficiency of differential diagnostic schemes for the determination of chronic hepatitis and liver cirrhosis of alcoholic and unspecified etiology, based on the determination of interleukin-13, alpha-1-defensin and enzymatic activities in blood serum. Material and method: The study included 41 patients with chronic hepatitis, 102 patients with liver cirrhosis of alcoholic and unspecified etiology. The control group included 43 donors from the blood transfusion station. The levels of enzymatic activities, interleukin-13 and alpha-1-defensin of blood serum were determined. Results: Hyaluronidase and elastase activities, as well as serum interleukin-13 and alpha-1-defensin, showed high diagnostic efficiency in detecting chronic diffuse inflammatory liver diseases, and trypsin-like and deoxyribonuclease activities in differential diagnosis of chronic hepatitis and liver cirrhosis. The developed differential diagnostic schemes allow differentiating chronic hepatitis and liver cirrhosis with a high odds ratio and have a high economic effect in comparison with Fibrotest and liver biopsy. The inclusion of interleukin-13 and alpha-1-defensin in the examination scheme allows to increase the diagnostic efficiency. Conclusions: Schemes for the differential diagnosis of chronic hepatitis and liver cirrhosis based on enzymatic activity, interleukin-13 and alpha-1-defensin of blood serum allow to improve the results of diagnostic measures, and also have a high economic effect in comparison with Fibrotest and liver biopsy.

Download Full-text