Polymicrobial pseudobacteraemias associated with non-sterile sodium citrate blood collection tubes

Summary Background This study aimed to verify whether blood drawn into six different commercial coagulation tubes generated comparable results of thrombin generation. Methods Blood was sequentially collected from 20 healthy subjects into different brand and draw volume 3.2% sodium citrate tubes (4.3 mL Sarstedt, 3.0 mL Greiner, 2.7 mL Becton Dickinson, 2.0 mL Kima, 1.8 mL Sarstedt and 1.0 mL Greiner). Thrombin generation was measured in plasma with the fully-automated ST Genesia analyzer using the weakest trigger (STG-BleedScreen). Results Different values of lag time (LT), time to reach thrombin peak (TP), thrombin peak height (PH) and endogenous thrombin potential (ETP) were commonly found in different tubes. Thrombin generation was the lowest in 4.3 mL Sarstedt tubes and the highest in 1.0 mL Greiner tubes. Other tubes displayed intermediate values. In multiple comparisons, LT was significantly different in 6/15 cases (40%), whilst PH, TP and ETP were significantly different in 14/15 (93%), 13/15 (87%) and 13/15 (87%) cases. The mean percent bias of LT, PH, TP and ETP ranged between -6% and +1%, -27% and +116%, -22% and +8%, and between -18% and +65%. The intra-assay imprecision of LT, PH, TP and ETP was exceeded in 0/15 (0%), 13/15 (87%), 6/15 (40%) and 13/15 (87%) comparisons. The correlation of LT, PH, TP and ETP values in different tubes ranged between 0.718–0.971, 0.570–0.966, 0.725–0.977 and 0.101–0.904. Conclusions Blood collection for thrombin generation assays requires local standardization using identical tubes for brand and draw volume, and reference ranges calculated according to type of tubes.

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Prothrombin Time Ratio Is Reduced by Magnesium Contamination in Evacuated Blood Collection Tubes

Thrombosis and Haemostasis ◽

10.1055/s-0037-1615647 ◽

2001 ◽

Vol 85 (04) ◽

pp. 647-650 ◽

Cited By ~ 12

Author(s):

W. van Dam ◽

A. Sturk ◽

R. M. Bertina ◽

A. M. H. P. van den Besselaar

Keyword(s):

Prothrombin Time ◽

Sodium Citrate ◽

Oral Anticoagulant Therapy ◽

Blood Collection ◽

Magnesium Ions ◽

Time Ratio ◽

Normal Plasma ◽

The Mean ◽

Blood Collection Tubes ◽

Prothrombin Time Ratio

SummaryMagnesium ions were detected in sodium citrate solutions in several lots of evacuated blood collection tubes. The mean concentrations ranged between 1.3 and 1.6 mmol/L. Magnesium was also present in the rubber stoppers of the blood collection tubes and could be leached into the citrate solution. It was shown that magnesium added to citrated plasma shortened the prothrombin time of both coumarin and normal plasma. The effect of magnesium was relatively greater on coumarin than on normal plasma resulting in reduced prothrombin time ratio. Shortening of the prothrombin time was also observed when magnesium chloride was added to dialysed plasma, i.e., in the absence of citrate. These results indicate that magnesium contamination can interfere with accurate INR determination in the control of oral anticoagulant therapy.

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Comparison of Samples Obtained From 3.2% Sodium Citrate Glass and Two 3.2% Sodium Citrate Plastic Blood Collection Tubes Used in Coagulation Testing

American Journal of Clinical Pathology ◽

10.1309/7v2kp1hp9q29bmn2 ◽

2004 ◽

Vol 122 (6) ◽

pp. 843-848 ◽

Cited By ~ 15

Author(s):

Robert C. Gosselin ◽

Kim Janatpour ◽

Edward C. Larkin ◽

Yanlap P. Lee ◽

John T. Owings

Keyword(s):

Sodium Citrate ◽

Blood Collection ◽

Coagulation Testing ◽

Blood Collection Tubes

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Comparison of Commercially Available Blood Collection Tubes Containing Sodium Citrate and Hirudin in Platelet Aggregation Testing

Medical Science Monitor Basic Research ◽

10.12659/msmbr.905246 ◽

2017 ◽

Vol 23 ◽

pp. 264-269 ◽

Cited By ~ 1

Author(s):

Walter J. Janse van Rensburg ◽

Patricia van der Merwe

Keyword(s):

Platelet Aggregation ◽

Sodium Citrate ◽

Blood Collection ◽

Blood Collection Tubes

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1-Desamino-8-D-Arginine Vasopressin (DDAVP) Infusion in Type IIB von Willebrand’s Disease: Shortening of Bleeding Time and Induction of a Variable Pseudothrombocytopenia

Thrombosis and Haemostasis ◽

10.1055/s-0038-1647265 ◽

1990 ◽

Vol 64 (01) ◽

pp. 117-120 ◽

Cited By ~ 22

Author(s):

Alessandra Casonato ◽

M Teresa Sartori ◽

Luigi de Marco ◽

Antonio Girolami

Keyword(s):

Monoclonal Antibody ◽

Platelet Count ◽

Arginine Vasopressin ◽

Calcium Concentration ◽

Sodium Citrate ◽

Bleeding Time ◽

Blood Collection ◽

Von Willebrand's Disease ◽

Blood Samples ◽

Von Willebrand’S Disease

SummaryWe have investigated the effects of 1-desamino-8-D-arginine vasopressin (DDAVP) infusion on platelet count and bleeding time in 4 patients with type IIB von Willebrand’s disease (vWd). Three of four patients showed a normalization of the bleeding time within 1 h after the infusion, while bleeding time was not modified in the fourth. In accordance with the literature, thrombocytopenia was observed after DDAVP infusion, but this thrombocytopenia was due to the anticoagulants used for blood collection. In two patients (F. I., G. F.) no thrombocytopenia was observed when platelets were counted by fingerstick method but there was a 20% platelet decrease in blood samples collected in sodium citrate and a 50% decrease in samples collected in EDTA. Dramatic falls in platelet counts (70–95%) were observed in the additional two patients (C. A., D.Z.) after DDAVP infusion, when both sodium citrate or EDTA were used as anticoagulants. In the latter two patients there was also a 50% decrease in platelet count when the fingerstick method was used. The decrease in the patient’s platelet count in EDTA samples after DDAVP infusion could be prevented, in part, by the previous additions of an anti GPIb monoclonal antibody and an anti GPIIb-IIIa monoclonal antibody.Thus, the thrombocytopenia observed in the four IIB vWd patients studied after DDAVP infusion seems to be, at least partially, a pseudothrombocytopenia depending on the calcium concentration in the blood samples and the availability of GPIb and GPIIb-IIIa receptors. These findings and the normalization of the bleeding time observed in three of the four patients has led us to reconsider the possible use of DDAVP in the treatment of our IIB vWd patients.

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