A comparative study of conventional versus new, magnesium-poor Vacutainer® Sodium Citrate blood collection tubes for determination of prothrombin time and INR

2014 ◽  
Vol 134 (1) ◽  
pp. 187-191 ◽  
Author(s):  
Anton M.H.P. van den Besselaar ◽  
Iris J.H. van Vlodrop ◽  
Paul B. Berendes ◽  
Christa M. Cobbaert
Keyword(s):  
Comparative Study ◽  
Prothrombin Time ◽  
Sodium Citrate ◽  
Blood Collection ◽  
Blood Collection Tubes ◽  
Citrate Blood

Polymicrobial pseudobacteraemias associated with non-sterile sodium citrate blood collection tubes

1993 ◽  
Vol 25 (4) ◽  
pp. 297-299 ◽  
Author(s):  
P.G. Rathbone ◽  
V. Sinickas ◽  
V. Humphery ◽  
S. Graves ◽  
A. Hellyar
Keyword(s):  
Sodium Citrate ◽  
Blood Collection ◽  
Blood Collection Tubes ◽  
Citrate Blood

Prothrombin Time Ratio Is Reduced by Magnesium Contamination in Evacuated Blood Collection Tubes

2001 ◽  
Vol 85 (04) ◽  
pp. 647-650 ◽  
Author(s):  
W. van Dam ◽  
A. Sturk ◽  
R. M. Bertina ◽  
A. M. H. P. van den Besselaar
Keyword(s):  
Prothrombin Time ◽  
Sodium Citrate ◽  
Oral Anticoagulant Therapy ◽  
Blood Collection ◽  
Magnesium Ions ◽  
Time Ratio ◽  
Normal Plasma ◽  
The Mean ◽  
Blood Collection Tubes ◽  
Prothrombin Time Ratio

SummaryMagnesium ions were detected in sodium citrate solutions in several lots of evacuated blood collection tubes. The mean concentrations ranged between 1.3 and 1.6 mmol/L. Magnesium was also present in the rubber stoppers of the blood collection tubes and could be leached into the citrate solution. It was shown that magnesium added to citrated plasma shortened the prothrombin time of both coumarin and normal plasma. The effect of magnesium was relatively greater on coumarin than on normal plasma resulting in reduced prothrombin time ratio. Shortening of the prothrombin time was also observed when magnesium chloride was added to dialysed plasma, i.e., in the absence of citrate. These results indicate that magnesium contamination can interfere with accurate INR determination in the control of oral anticoagulant therapy.

Increased plasma glucose levels after change of recommendation from NaF to citrate blood collection tubes

2014 ◽  
Vol 47 (7-8) ◽  
pp. 625-628 ◽  
Author(s):  
Peter Ridefelt ◽  
Torbjörn Åkerfeldt ◽  
Johanna Helmersson-Karlqvist
Keyword(s):  
Plasma Glucose ◽  
Blood Collection ◽  
Glucose Levels ◽  
Blood Collection Tubes ◽  
Citrate Blood

Evacuated blood collection tubes and the determination of beta-thromboglobulin

1981 ◽  
Vol 23 (3) ◽  
pp. 323-324 ◽  
Author(s):  
N. Eche ◽  
A. Boneu ◽  
P. Sie ◽  
H. Lucot
Keyword(s):  
Blood Collection ◽  
Blood Collection Tubes

scholarly journals Comparative Study of Blood Collection Tubes and Thromboplastin Reagents for Correction of INR Discrepancies

2012 ◽  
Vol 138 (2) ◽  
pp. 248-254 ◽  
Author(s):  
Anton M.H.P. van den Besselaar ◽  
Anton P. van Zanten ◽  
Helen M. Brantjes ◽  
Marc G.L.M. Elisen ◽  
Felix J.M. van der Meer ◽  
...  
Keyword(s):  
Comparative Study ◽  
Blood Collection ◽  
Blood Collection Tubes

scholarly journals Thrombin generation in different commercial sodium citrate blood tubes

2019 ◽  
Vol 0 (0) ◽  
Author(s):  
Gian Luca Salvagno ◽  
Davide Demonte ◽  
Matteo Gelati ◽  
Giovanni Poli ◽  
Emmanuel J. Favaloro ◽  
...  
Keyword(s):  
Thrombin Generation ◽  
Sodium Citrate ◽  
Multiple Comparisons ◽  
Lag Time ◽  
Peak Height ◽  
Blood Collection ◽  
Reference Ranges ◽  
Becton Dickinson ◽  
The Mean ◽  
Citrate Blood

Summary Background This study aimed to verify whether blood drawn into six different commercial coagulation tubes generated comparable results of thrombin generation. Methods Blood was sequentially collected from 20 healthy subjects into different brand and draw volume 3.2% sodium citrate tubes (4.3 mL Sarstedt, 3.0 mL Greiner, 2.7 mL Becton Dickinson, 2.0 mL Kima, 1.8 mL Sarstedt and 1.0 mL Greiner). Thrombin generation was measured in plasma with the fully-automated ST Genesia analyzer using the weakest trigger (STG-BleedScreen). Results Different values of lag time (LT), time to reach thrombin peak (TP), thrombin peak height (PH) and endogenous thrombin potential (ETP) were commonly found in different tubes. Thrombin generation was the lowest in 4.3 mL Sarstedt tubes and the highest in 1.0 mL Greiner tubes. Other tubes displayed intermediate values. In multiple comparisons, LT was significantly different in 6/15 cases (40%), whilst PH, TP and ETP were significantly different in 14/15 (93%), 13/15 (87%) and 13/15 (87%) cases. The mean percent bias of LT, PH, TP and ETP ranged between -6% and +1%, -27% and +116%, -22% and +8%, and between -18% and +65%. The intra-assay imprecision of LT, PH, TP and ETP was exceeded in 0/15 (0%), 13/15 (87%), 6/15 (40%) and 13/15 (87%) comparisons. The correlation of LT, PH, TP and ETP values in different tubes ranged between 0.718–0.971, 0.570–0.966, 0.725–0.977 and 0.101–0.904. Conclusions Blood collection for thrombin generation assays requires local standardization using identical tubes for brand and draw volume, and reference ranges calculated according to type of tubes.

scholarly journals Impact of various reagents during blood collection from poultry to analyze morpho-biochemical indicators

2021 ◽  
Vol 262 ◽  
pp. 02009
Author(s):  
Vladimir Vertiprakhov ◽  
Alena Grozina ◽  
Vladimir Fisinin ◽  
Natalya Ovchinnikova ◽  
Anatoly Bittirov
Keyword(s):  
Broiler Chickens ◽  
Blood Count ◽  
Sodium Citrate ◽  
Medical Science ◽  
White Blood Count ◽  
Blood Collection ◽  
Blood Samples ◽  
Biochemical Indicators ◽  
The Impact

The work provides experimental data on the impact of anticoagulants (K3 - EDTA, 3.8% sodium citrate and lithium heparin) and coagulation activator on morpho-biochemical indicators in the blood samples of broiler chickens of cross-selection of Agricultural Center “Smena” at the age of 35 days. The studies were carried out with the use of semi-automatic flow analyzer Sinnowa BS-3000P (SINNOWA Medical Science & Technology Co., Ltd, China) and veterinary automatic blood analyzer DF-50 by Dymind Biotech (PRC) with the use of original reagents. The results showed that the most optimal reagent for determination of morphological indicators of the poultry blood was EDTA. The average white blood count (WBC) is lower in blood samples with citrate and heparin in contrast to EDTA by 30.5% and 24.1%, respectively. The average red blood count (RBC) is lower in tubes with sodium citrate (by 37.9 %) and lithium heparin (by 13.8 %) in contrast to K3-EDTA. When the sodium citrate anticoagulant is used, the blood biochemistry results for all positions of the experiment, excluding calcium, are lower than in heparin, and its closest values are lower than in coagulation activator, excluding trypsin activity. That’s why, the most optimal reagents for biochemistry, including for blood enzyme tests, should be considered the tubes with heparin or coagulation activator.

scholarly journals Next-generation rapid serum tube technology using prothrombin activator coagulant: fast, high-quality serum from normal samples

2019 ◽  
Vol 57 (4) ◽  
pp. 483-497 ◽  
Author(s):  
Kong-Nan Zhao ◽  
Goce Dimeski ◽  
John de Jersey ◽  
Lambro A. Johnson ◽  
Michael Grant ◽  
...  
Keyword(s):  
Whole Blood ◽  
Blood Clotting ◽  
Blood Collection ◽  
High Quality ◽  
Coagulation Activity ◽  
Novel Approach ◽  
Normal Individuals ◽  
Rapid Generation ◽  
Blood Collection Tubes

AbstractBackgroundIncomplete blood clotting or latent clotting in serum is a common laboratory problem, especially for patients on anticoagulant therapy or when serum tubes are centrifuged before clotting is completed. We describe a novel approach to producing high-quality serum using snake venom prothrombin activator complex (OsPA) as an additive in blood collection tubes for non-anticoagulated (normal) individuals.MethodsPlasma clotting assays were performed using a Hyland-Clotek instrument. Blood clotting was visually observed, and thromboelastography was also performed to determine the important parameters of coagulation. Thrombin generation was assayed using the chromogenic substrate S-2238, and biochemical analytes in the serum were determined on chemistry and immunoassay analysers. Fibrinogen was determined by either ELISA or Clauss fibrinogen assay.ResultsWe initially showed that OsPA had strong coagulation activity in clotting not only recalcified citrated plasma and recalcified citrated whole blood, but also fresh whole blood in a clinical setting. The use of TEG clearly showed improved speed of clotting and generation of a firmer clot. We also showed that the use of OsPA to produce serum did not interfere with the determination of commonly measured biochemical analytes. The underlying clotting mechanism involves a burst of thrombin production at the initial stages of the clotting process upon contact with prothrombin in blood.ConclusionsThese results demonstrate rapid generation of high-quality serum, contributing to faster turnaround times with standardised quality samples, for accurate analyte determinations in normal individuals.

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