Expression of the A12 form of acetylcholinesterase by developing avian leg muscle cells in vivo and during differentiation in primary cell cultures

Abstract Oxygen (O2) is an essential element for aerobic respiration. Atmospheric concentration of O2 is approximately 21%. Mammalian cells, however, are generally adapted to O2 levels much lower than atmospheric conditions. The pericellular levels of O2 must also be maintained within a fairly narrow range to meet the demands of cells. This applies equally to cells in vivo and cells in primary cultures. There has been growing interest in the performance of cell culture experiments under various O2 levels to study molecular and cellular responses. To this end, a range of technologies (e.g. gas-permeable technology) and instruments (e.g. gas-tight boxes and gas-controlled incubators) have been developed. It should be noted, however, that some of these have limitations and they are still undergoing refinement. Nevertheless, better results should be possible when technical concerns are taken into account. This paper aims to review various aspects of O2 level adjustment in primary cell cultures, regulation of pericellular O2 gradients and possible effects of the cell culture medium.

Download Full-text

Replication of Coxsackievirus B3 in Primary Cell Cultures Generates Novel Viral Genome Deletions

Journal of Virology ◽

10.1128/jvi.01774-07 ◽

2007 ◽

Vol 82 (4) ◽

pp. 2033-2037 ◽

Cited By ~ 37

Author(s):

K.-S. Kim ◽

N. M. Chapman ◽

S. Tracy

Keyword(s):

Cell Cultures ◽

Viral Genome ◽

Coxsackievirus B3 ◽

Primary Cell ◽

Primary Cell Cultures ◽

Deletion Size ◽

Cellular Environment ◽

Immortalized Cell ◽

Selection Of

ABSTRACT Coxsackievirus B3 (CVB3) generates 5′-terminally deleted genomes (TDs) during replication in murine hearts. We show here that CVB3 populations with TDs can also be generated within two to three passages of CVB3 in primary, but not immortalized, cell cultures. Deletions of less than 49 nucleotides increase in size during passage, while 5′ TDs of 49 nucleotides appear to be the maximum deletion size. The cellular environment of contact-inhibited primary cell cultures or the myocardium in vivo is sufficient for the selection of 5′ TDs over undeleted genomes.

Download Full-text

Osservazioni su Generazioni Successive di Tumori Prodotti in Criceti con Inoculo di Cellule Trasformate da SV40

Tumori Journal ◽

10.1177/030089167205800204 ◽

1972 ◽

Vol 58 (2) ◽

pp. 95-106 ◽

Cited By ~ 1

Author(s):

José Menezes

Keyword(s):

Lymph Node ◽

Cell Cultures ◽

Tumor Biology ◽

T Antigen ◽

Primary Cell ◽

Transformed Cells ◽

Local Growth ◽

Primary Cell Cultures ◽

Successive Generations

This paper describes some important features of successive generations of tumors produced in hamsters by inoculation of SV40-transformed cells (C12TSV5 and RHaT), and presents data indicating the correlation among increased percentage of polykaryocytes in the tumor primary cell cultures, increased capacity of cells for fusion, and the production of metastases. According to our knowledge this correlation has not yet been described in tumor biology. The data presented also suggest that the large polykaryocytes from tumor primary cell cultures do not multiply and that they are particularly affected by most experimental procedures. Thus they appear to perish or be drastically reduced in cultures of cells submitted to centrifugation, freezing procedures for routine cells preservation and even trypsination. Our observations also show that cell morphology, SV40 rescuability, and T-antigen appear to be among the features which are not lost in transformed cells throughout in vivo passages. Furthermore, a theory is discussed in an attempt to explain, in part, the local growth of the tumor as well as the occurrence of metastases in the hamster, particularly those at the lymph node level.

Download Full-text