HLA-B27 alleles in Japanese patients with ankylosing spondylitis (AS) and healthy controls

1995 ◽  
Vol 44 ◽  
pp. 46
Keyword(s):  
Ankylosing Spondylitis ◽  
Japanese Patients ◽  
Healthy Controls ◽  
Hla B27

HLA-B27 subtypes in Turkish patients with ankylosing spondylitis and healthy controls

2011 ◽  
Vol 32 (10) ◽  
pp. 3103-3105 ◽  
Author(s):  
Muradiye Acar ◽  
Tulin Cora ◽  
Recep Tunc ◽  
Hasan Acar
Keyword(s):  
Ankylosing Spondylitis ◽  
Healthy Controls ◽  
Hla B27 ◽  
Turkish Patients

Association of hla-b39 with hla-b27-negative ankylosing spondylitis and pauciarticular juvenile rheumatoid arthritis in japanese patients

1995 ◽  
Vol 38 (11) ◽  
pp. 1672-1677 ◽  
Author(s):  
Akihiro Yamaguchi ◽  
Naoyuki Tsuchiya ◽  
Hiroshi Mitsui ◽  
Michiko Shiota ◽  
Atsuko Ogawa ◽  
...  
Keyword(s):  
Rheumatoid Arthritis ◽  
Ankylosing Spondylitis ◽  
Juvenile Rheumatoid Arthritis ◽  
Japanese Patients ◽  
Hla B27

scholarly journals Antibody activity in ankylosing spondylitis sera to two sites on HLA B27.1 at the MHC groove region (within sequence 65-85), and to a Klebsiella pneumoniae nitrogenase reductase peptide (within sequence 181-199).

1990 ◽  
Vol 171 (5) ◽  
pp. 1635-1647 ◽  
Author(s):  
C Ewing ◽  
R Ebringer ◽  
G Tribbick ◽  
H M Geysen
Keyword(s):  
Amino Acids ◽  
Ankylosing Spondylitis ◽  
Klebsiella Pneumoniae ◽  
Homologous Sequence ◽  
Antibody Activity ◽  
Healthy Controls ◽  
Hla B27 ◽  
Reactive Site ◽  
A Site ◽  
Groove Region

74 overlapping peptides of varying lengths from Klebsiella pneumoniae nitrogenase reductase (residues 181-199) and from the HLA B27.1 molecule (residues 65-85) were synthesized and tested by ELISA against sera from HLA B27+ ankylosing spondylitis (AS) patients, and sera from HLA B27+ and HLA B27- healthy first-degree relatives. Antibody activity in AS sera to Klebsiella peptides of four to eight amino acids was maximal with the peptide NSRQTDR. Activity to HLA B27 peptides was maximal with the peptide KAKAQTDR (named epitope I). These peptides overlap with, but are proximal to the NH2 terminus from QTDRED, which is homologous in HLA B27.1 and K. pneumoniae nitrogenase reductase. A second weaker reactive site was noted in the HLA B27.1 peptides, proximal to the COOH terminus from the homologous sequence, namely peptide REDLRTLL (named epitope II). Little activity was seen against peptides that included the entire homologous sequence. Sera from 50 AS patients showed higher total Ig activity against peptides KAKAQTDR (p less than 0.001) and NSRQTDR (p less than 0.02) than did sera from 22 B27+ and 22 B27- healthy controls. These data indicate that AS patient sera contain antibodies that bind to K. pneumoniae nitrogenase peptides and HLA B27.1 peptides, and that there are at least two epitopes on HLA B27.1 in the alpha 1 domain, at the MHC groove region, that are autoantigenic in AS patients. Epitope I may be a site for crossreactivity between HLA B27 and Klebsiella.

A high density SNP genotyping approach within the 19q13 chromosome region identifies an association of a CNOT3 polymorphism with ankylosing spondylitis

2012 ◽  
Vol 71 (5) ◽  
pp. 714-717 ◽  
Author(s):  
Roberto Díaz-Peña ◽  
Ana M Aransay ◽  
Beatriz Suárez-Álvarez ◽  
Jacome Bruges-Armas ◽  
Naiara Rodríguez-Ezpeleta ◽  
...  
Keyword(s):  
Ankylosing Spondylitis ◽  
High Throughput ◽  
Human Leucocyte Antigen ◽  
Chromosome Region ◽  
Healthy Controls ◽  
Nucleotide Polymorphisms ◽  
Hla B27 ◽  
Single Nucleotide ◽  
Sliding Windows ◽  
Genomic Variants

ObjectiveTo identify genomic variants in the 19q13 chromosome region associated with ankylosing spondylitis (AS) in human leucocyte antigen (HLA)-B27-positive populations.MethodsHigh-throughput genotyping of 1536 haplotype-tag single nucleotide polymorphisms (SNPs) was performed in 249 patients with AS and 302 healthy controls. Some of the identified associations were validated by genotyping four SNPs in two additional cohorts consisting of 412 cases/301 controls and 144 cases/203 controls. All individuals selected (both cases and controls) were HLA-B27-positive.ResultsTwo markers in two different genes (CNOT3 and LAIR2) showed significant association (p<10−3) with AS. In addition, sliding windows analysis showed association of groups of adjacent SNPs in regions located around CNOT3 (Chr19: 59347459-59356564, p=2.43×10−4 to 6.54×10−4). The associations were validated by genotyping four SNPs from regions located near LAIR2 and CNOT3 genes (rs1055234, rs8111398, rs2287828 and rs4591276) in two additional cohorts. The CNOT3 polymorphism (rs1055234) remained associated with AS (combined p=9.73×10−6). One SNP, located downstream of KIR3DL1, was detected which, tested in combination with HLA-Bw4I80, was associated with AS.ConclusionA novel significant association was detected between SNP rs1055234 and AS susceptibility.

scholarly journals Shotgun metagenomics reveals an enrichment of potentially cross-reactive bacterial epitopes in ankylosing spondylitis patients, as well as the effects of TNFi therapy upon microbiome composition

2019 ◽  
Vol 79 (1) ◽  
pp. 132-140 ◽  
Author(s):  
Jian Yin ◽  
Peter Richard Sternes ◽  
Mingbang Wang ◽  
Jing Song ◽  
Mark Morrison ◽  
...  
Keyword(s):  
Ankylosing Spondylitis ◽  
Gut Microbiome ◽  
Bacterial Species ◽  
Metagenomic Sequencing ◽  
Healthy Controls ◽  
Hla B27 ◽  
Clinical Genetic ◽  
Shotgun Metagenomics ◽  
Microbiome Composition ◽  
Shotgun Metagenomic Sequencing

ObjectivesDiverse evidence including clinical, genetic and microbiome studies support a major role of the gut microbiome in the common immune-mediated arthropathy, ankylosing spondylitis (AS). We set out to (1) further define the key microbial characteristics driving disease, and (2) examine the effects of tumour necrosis factor-inhibitor (TNFi) therapy upon the microbiome.MethodsThe stools from a case–control cohort of 250 Han-Chinese subjects underwent shotgun metagenomic sequencing. All subjects were genotyped using the Illumina CoreExome SNP microarray.ResultsPrevious reports of gut dysbiosis in AS were reconfirmed and several notable bacterial species and functional categories were differentially abundant. TNFi therapy was correlated with a restoration the perturbed microbiome observed in untreated AS cases to that of healthy controls, including several important bacterial species that have been previously associated with AS and other related diseases. Enrichment of bacterial peptides homologous to HLA-B27-presented epitopes was observed in the stools of patients with AS, suggesting that either HLA-B27 fails to clear these or that they are involved in driving HLA-B27-associated immune reactions. TNFi therapy largely restored the perturbed microbiome observed in untreated AS cases to that of healthy controls, including several important bacterial species that have been previously associated with AS and other related diseases. TNFi therapy of patients with AS was also associated with a reduction of potentially arthritogenic bacterial peptides, relative to untreated patients.ConclusionThese findings emphasise the key role that the gut microbiome plays in driving the pathogenesis of AS and highlight potential therapeutic and/or preventative targets.

HLA-BW35 AND B5 IN JAPANESE PATIENTS WITH GRAVES' DISEASE

1977 ◽  
Vol 86 (4) ◽  
pp. 754-757 ◽  
Author(s):  
A. Kawa ◽  
S. Nakamura ◽  
M. Nakazawa ◽  
S. Sakaguchi ◽  
T. Kawabata ◽  
...  
Keyword(s):  
Japanese Patients ◽  
Graves Disease ◽  
Healthy Controls

ABSTRACT Thirty-three Japanese patients with Graves' disease and 106 healthy controls living in the Kagoshima area, the southernmost part of the Japanese mainland, were HLA typed by the NIH method. None of them were related to each other. The only antigen showing an increased frequency in Japanese patients with Graves' disease was HLA-BW35 (corrected P < 0.02). A decreased frequency of B5 in the patients was also statistically significant (corrected P < 0.02).

Sign in / Sign up

Export Citation Format

Share Document