cDNA sequencing based typing of HLA-A alleles using a robotic workstation

1996 ◽  
Vol 47 (1-2) ◽  
pp. 115
Author(s):  
F. Fischer Gottfried ◽  
Faé Ingrid ◽  
Petrasek Monika ◽  
Broer Edeltraud ◽  
R. Mayr Wolfgang
Keyword(s):  
Cdna Sequencing ◽  
Robotic Workstation

The Hierarchic Order of Intermediate Filament Structure and Assembly

1985 ◽  
Vol 43 ◽  
pp. 722-725
Author(s):  
U. Aebi ◽  
E.C. Glavaris ◽  
R. Eichner
Keyword(s):  
Tissue Specificity ◽  
Structural Model ◽  
Eukaryotic Cells ◽  
Cdna Sequencing ◽  
Filament Structure ◽  
Keratin Filaments ◽  
Isoelectric Points ◽  
Immunological Crossreactivity

Five different classes of intermediate-sized filaments (IFs) have been identified in differentiated eukaryotic cells: vimentin in mesenchymal cells, desmin in muscle cells, neurofilaments in nerve cells, glial filaments in glial cells and keratin filaments in epithelial cells. Despite their tissue specificity, all IFs share several common attributes, including immunological crossreactivity, similar morphology (e.g. about 10 nm diameter - hence ‘10-nm filaments’) and the ability to reassemble in vitro from denatured subunits into filaments virtually indistinguishable from those observed in vivo. Further more, despite their proteinchemical heterogeneity (their MWs range from 40 kDa to 200 kDa and their isoelectric points from about 5 to 8), protein and cDNA sequencing of several IF polypeptides (for refs, see 1,2) have provided the framework for a common structural model of all IF subunits.

scholarly journals UNAGI: an automated pipeline for nanopore full-length cDNA sequencing uncovers novel transcripts and isoforms in yeast

2020 ◽  
Vol 20 (4) ◽  
pp. 523-536
Author(s):  
Mohamad Al kadi ◽  
Nicolas Jung ◽  
Shingo Ito ◽  
Shoichiro Kameoka ◽  
Takashi Hishida ◽  
...  
Keyword(s):  
Full Length ◽  
Cdna Sequencing ◽  
Full Length Cdna ◽  
Novel Transcripts ◽  
Automated Pipeline

scholarly journals Long-read cDNA sequencing identifies functional pseudogenes in the human transcriptome

2021 ◽  
Vol 22 (1) ◽  
Author(s):  
Robin-Lee Troskie ◽  
Yohaann Jafrani ◽  
Tim R. Mercer ◽  
Adam D. Ewing ◽  
Geoffrey J. Faulkner ◽  
...  
Keyword(s):  
Cultured Cells ◽  
Open Reading Frames ◽  
Cdna Sequencing ◽  
Protein Coding ◽  
Dynamic Component ◽  
Gene Copies ◽  
Long Read ◽  
Normal Human ◽  
Reading Frames ◽  
Transcriptional Landscape

AbstractPseudogenes are gene copies presumed to mainly be functionless relics of evolution due to acquired deleterious mutations or transcriptional silencing. Using deep full-length PacBio cDNA sequencing of normal human tissues and cancer cell lines, we identify here hundreds of novel transcribed pseudogenes expressed in tissue-specific patterns. Some pseudogene transcripts have intact open reading frames and are translated in cultured cells, representing unannotated protein-coding genes. To assess the biological impact of noncoding pseudogenes, we CRISPR-Cas9 delete the nucleus-enriched pseudogene PDCL3P4 and observe hundreds of perturbed genes. This study highlights pseudogenes as a complex and dynamic component of the human transcriptional landscape.

Automation ofIn situHybridization: Application of the Capillary Action Robotic Workstation

1988 ◽  
Vol 11 (4) ◽  
pp. 253-258 ◽  
Author(s):  
Elizabeth R. Unger ◽  
David J. Brigati ◽  
Margie L. Chenggis ◽  
Lynn Rae Budgeon ◽  
Douglas Koebler ◽  
...  
Keyword(s):  
Capillary Action ◽  
Robotic Workstation

scholarly journals Human-Centered Robotic Development in the Steel Shop: Improving Health, Safety and Digital Skills at the Workplace

Metals ◽  
2021 ◽  
Vol 11 (4) ◽  
pp. 647
Author(s):  
Valentina Colla ◽  
Ruben Matino ◽  
Antonius Johannes Schröder ◽  
Mauro Schivalocchi ◽  
Lea Romaniello
Keyword(s):  
Social Innovation ◽  
Health And Safety ◽  
Field Evaluation ◽  
End Users ◽  
Operating Conditions ◽  
Viable Solution ◽  
Relevant Role ◽  
Steel Sector ◽  
Robot Cooperation ◽  
Robotic Workstation

Within the implementation of the Industry 4.0 paradigm in the steel sector, robots can play a relevant role in improving health and safety conditions at the workplace, by overtaking cumbersome, repetitive and risky operations. However, the implementation of robotics solutions in this particular sector is hampered by harsh operating conditions and by particular features of many procedures, which require a combination of force and sensitivity. Human–robot cooperation is a viable solution to overcome existing barriers, by synergistically combining human and robot abilities in the sense of a human-centered Industry 5.0. In this sense, robotics solution should be designed in a way to integrate and meet the end-users’ demands in a common development process for successfully implementation and widely acceptance. The paper presents the outcomes of the field evaluation of a robotic workstation, which was designed for a complex maintenance operation that is daily performed in the steel shop. The system derives from a co-creation process, where workers were involved since the beginning in the design process, according to the paradigm of social innovation combining technological and social development. Therefore, the evaluation aimed at assessing both system reliability and end-users’ satisfaction. The results show that the human-centered robotic workstations are successful in reducing cumbersome operations and improving workers’ health and safety conditions, and that this fact is clearly perceived by system users and developers.

scholarly journals Genome and transcriptome of a pathogenic yeast, Candida nivariensis

2021 ◽  
Author(s):  
Yunfan Fan ◽  
Andrew N Gale ◽  
Anna Bailey ◽  
Kali Barnes ◽  
Kiersten Colotti ◽  
...  
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Candida Glabrata ◽  
Nanopore Sequencing ◽  
Cdna Sequencing ◽  
Pathogenic Yeast ◽  
Mitochondrial Sequence ◽  
Dna And Rna ◽  
Oxford Nanopore ◽  
Candida Nivariensis ◽  
Oxford Nanopore Technologies

Abstract We present a highly contiguous genome and transcriptome of the pathogenic yeast, Candida nivariensis. We sequenced both the DNA and RNA of this species using both the Oxford Nanopore Technologies (ONT) and Illumina platforms. We assembled the genome into an 11.8 Mb draft composed of 16 contigs with an N50 of 886 Kb, including a circular mitochondrial sequence of 28 Kb. Using direct RNA nanopore sequencing and Illumina cDNA sequencing, we constructed an annotation of our new assembly, supplemented by lifting over genes from Saccharomyces cerevisiae and Candida glabrata.

Induction of metallothionein and other mRNA species by carcinogens and tumor promoters in primary human skin fibroblasts

1986 ◽  
Vol 6 (5) ◽  
pp. 1760-1766
Author(s):  
P Angel ◽  
A Pöting ◽  
U Mallick ◽  
H J Rahmsdorf ◽  
M Schorpp ◽  
...  
Keyword(s):  
Uv Light ◽  
Human Fibroblasts ◽  
Nucleic Acid Hybridization ◽  
Tumor Promoter ◽  
Cdna Clones ◽  
Tumor Promoters ◽  
Human Skin Fibroblasts ◽  
Cdna Sequencing ◽  
Transcript Levels ◽  
Inducible Protein

We used nucleic acid hybridization and cDNA cloning techniques to isolate human sequences that respond to the tumor promoter 12-O-tetradecanoylphorbol-13-acetate (TPA). These clones were used as probes to examine changes of gene expression that occurred after the proliferation of exponentially growing primary human fibroblasts was arrested. Transcript levels detected by these probes were increased coordinately by treatment of the cells with UV light, mitomycin C, TPA, or the UV light-induced extracellular protein synthesis-inducing factor EPIF (M. Schorpp, U. Mallick, H. J. Rahmsdorf, and P. Herrlich, Cell 37:861-868, 1984). Proteins coded for by these transcripts were characterized by hybrid-promoted translation and by cDNA sequencing. One of the cDNA clones was homologous to the metallothionein IIa gene, and one set of related clones selected RNA for the secreted TPA-inducible protein XHF1 (U. Mallick, H. J. Rahmsdorf, N. Yamamoto, H. Ponta, R.-D. Wegner, and P. Herrlich, Proc. Natl. Acad. Sci. USA 79:7886-7890, 1982).

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