TO FLOW CYTOMETRY FROM IMAGE ANALYSIS THROUGH ANALYTICAL AND PREPARATIVE IMAGE CYTOMETRY BY AN ADHERENT CELL ANALYSIS AND SORTING CYTOMETER

AbstractBiofilms are microbial communities that represent a highly abundant form of microbial life on Earth. Inside biofilms, phenotypic and genotypic variations occur in three-dimensional space and time; microscopy and quantitative image analysis are therefore crucial for elucidating their functions. Here, we present BiofilmQ—a comprehensive image cytometry software tool for the automated and high-throughput quantification, analysis and visualization of numerous biofilm-internal and whole-biofilm properties in three-dimensional space and time.

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First estimates of genome size in ribbon worms (phylum Nemertea) using flow cytometry and Feulgen image analysis densitometry

Canadian Journal of Zoology ◽

10.1139/cjz-2014-0068 ◽

2014 ◽

Vol 92 (10) ◽

pp. 847-851 ◽

Cited By ~ 3

Author(s):

Kelly L. Mulligan ◽

Terra C. Hiebert ◽

Nicholas W. Jeffery ◽

T. Ryan Gregory

Keyword(s):

Flow Cytometry ◽

Image Analysis ◽

Body Size ◽

Genome Size ◽

Positive Relationship ◽

Nuclear Dna ◽

Nuclear Dna Content ◽

Size Estimation ◽

Size Diversity ◽

Size Estimates

Ribbon worms (phylum Nemertea) are among several animal groups that have been overlooked in past studies of genome-size diversity. Here, we report genome-size estimates for eight species of nemerteans, including representatives of the major lineages in the phylum. Genome sizes in these species ranged more than fivefold, and there was some indication of a positive relationship with body size. Somatic endopolyploidy also appears to be common in these animals. Importantly, this study demonstrates that both of the most common methods of genome-size estimation (flow cytometry and Feulgen image analysis densitometry) can be used to assess genome size in ribbon worms, thereby facilitating additional efforts to investigate patterns of variability in nuclear DNA content in this phylum.

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3P339 Development of Cell Analysis and Separation System by Ultra Fast Image Analysis(Bioengineering,The 48th Annual Meeting of the Biophysical Society of Japan)

Seibutsu Butsuri ◽

10.2142/biophys.50.s205_1 ◽

2010 ◽

Vol 50 (supplement2) ◽

pp. S205

Author(s):

Akihiro Hattori ◽

Masahito Hayashi ◽

Shin'ichi Ishiwata ◽

Kenji Yasuda

Keyword(s):

Image Analysis ◽

Annual Meeting ◽

Cell Analysis ◽

Separation System ◽

Biophysical Society

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Additional Techniques in Serous Effusions

Analytical Cellular Pathology ◽

10.1155/2002/376731 ◽

2002 ◽

Vol 24 (1) ◽

pp. 1-4 ◽

Cited By ~ 4

Author(s):

A. G. J. M. Hanselaar

Keyword(s):

Flow Cytometry ◽

Malignant Mesothelioma ◽

Peritoneal Cavity ◽

Diagnostic Tool ◽

Image Cytometry ◽

Serous Effusion ◽

Cytological Examination ◽

Valuable Diagnostic Tool ◽

Carcinoma Of The Ovary ◽

Dna Image Cytometry

Cytological examination is a valuable diagnostic tool in case of a serous effusion. The firstmanifestation of malignancy may be an effusion of the pleural, pericardial, or peritoneal cavity, especially in carcinoma of the ovary, or lung, and malignant mesothelioma. In other malignancies effusions may occur in the course of the disease. The contribution by Motherby et al. in this issue of ACP focuses on the contribution of image and flow cytometry to establish the presence or absence of malignancy in serous effusions [16]. They point out that the sensitivity of DNA image cytometry in equivocal effusions may be as high as 87.5%, and that for the detection of malignancy, DNA image cytometry is superior to flow cytometry.

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A Telepathology Based Virtual Reference and Certification Centre for DNA Image Cytometry

Analytical Cellular Pathology ◽

10.1155/2000/506561 ◽

2000 ◽

Vol 21 (3-4) ◽

pp. 149-159 ◽

Cited By ~ 2

Author(s):

G. Haroske ◽

Francoise Giroud ◽

K. D. Kunze ◽

W. Meyer

Keyword(s):

Image Analysis ◽

Clinical Application ◽

Biological Markers ◽

Quantitative Methods ◽

Image Cytometry ◽

Virtual Reference ◽

Reference Center ◽

To Come ◽

Fast Evolution ◽

Dna Image Cytometry

An increasing need for flexible consultation between pathologists, including the application of fast evolving supplementary technologies, has been identified during the last years. Although pathology is already one of the most advanced application of telemedicine there is more to come from the fast evolution towards computerized microscope image analysis: A reproducible quantification of measurable descriptors of the lesions in cells and tissues (so‐called biological markers) is an indispensable adjunct to routine diagnostic application. Among such quantitative methods DNA image cytometry is increasingly applied by pathologists for assistance in diagnostics.As for other pathological issues, too, a reference center for the clinical application of DNA image cytometry might be therefore of utmost value for pathologists using that method.Based on advanced telematic technologies, a Virtual Reference and Certification Center (VRCC) could be installed for certifying the cytometry hardware and software, the analytical procedures, and the basic interpretation of the results. It will be designed to be operated as a non‐attended service, based on quantification servers accessible via Internet round the clock. The VRCC will supply appropriate standardization and normalization materials and run a GroupWare platform for consensus making by experts.

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Touch Imprint Intraoperative Flow Cytometry as a Complementary Tool for Detailed Assessment of Resection Margins and Tumor Biology in Liver Surgery for Primary and Metastatic Liver Neoplasms

Methods and Protocols ◽

10.3390/mps4030066 ◽

2021 ◽

Vol 4 (3) ◽

pp. 66

Author(s):

Georgios S. Markopoulos ◽

Georgios K. Glantzounis ◽

Anna C. Goussia ◽

Georgios D. Lianos ◽

Anastasia Karampa ◽

...

Keyword(s):

Flow Cytometry ◽

Dna Content ◽

Liver Tumors ◽

Tumor Biology ◽

Surgical Margins ◽

Distribution Analysis ◽

Cell Analysis ◽

Term Survival ◽

Detailed Assessment ◽

Metastatic Liver

Liver resection is the main treatment for primary and metastatic liver tumors in order to achieve long-term survival with good quality of life. The ultimate goal of surgical oncology is to achieve complete tumor removal with adequate clear surgical margins. Flow cytometry is a powerful analytical technique with applications such as phenotypic analysis and quantification of DNA content. Intraoperative flow cytometry (iFC) is the application of flow cytometry for DNA content/ploidy and cell cycle distribution analysis during surgery for tumor cell analysis and margin evaluation. It has been used for cell analysis of intracranial tumors and recently of head and neck carcinomas and breast carcinomas, as well as for tumor margin evaluation. Herein, we present a novel touch imprint iFC protocol for the detailed assessment of tumor margins during excision of malignant hepatic lesions. The protocol aims to offer information on surgical margins after removal of malignant liver tumors based on DNA content of cancer cells and to corroborate the results of iFC with that of histopathological analysis. Based on the established role of iFC in other types of malignancies, our specialized protocol has the potential, through characterization of cells in liver transection surface post hepatectomy, to offer significant information on the type of resection and tumor biology. This information can be used to effectively guide intra- and postoperative patient management.

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