Effect of pregnant mare serum gonadotropin on the induction and degradation of FSH and LH receptors in the granulosa cell of the immature rat

1984 ◽  
Vol 37 (2) ◽  
pp. 215-222 ◽  
Author(s):  
N. Vidyashankar ◽  
N.R. Moudgal
Keyword(s):  
Granulosa Cell ◽  
Immature Rat ◽  
Pregnant Mare Serum Gonadotropin ◽  
Serum Gonadotropin

scholarly journals Ability of human chorionic gonadotropin β-subunit to inhibit the steroidogenic response to lutropin

1979 ◽  
Vol 180 (3) ◽  
pp. 573-578 ◽  
Author(s):  
R R Dighe ◽  
K Muralidhar ◽  
N R Moudgal
Keyword(s):  
Chorionic Gonadotropin ◽  
Human Chorionic Gonadotropin ◽  
Beta Subunit ◽  
Alpha Subunit ◽  
Immature Rat ◽  
Progesterone Production ◽  
Pregnant Mare Serum Gonadotropin ◽  
Serum Gonadotropin ◽  
Constant Dose ◽  
Human Chorionic Gonadotropin Beta

Ability of the beta-subunit of human chorionic gonadotropin to inhibit the response to lutropin (luteinizing hormone, LH) was tested in the immature rat ovarian system and pregnant-mare-serum-gonadotropin-primed rat ovarian system with progesterone production being used as the response. Human chorionic gonadotropin beta-subunit was found to inhibit human and ovine lutropin-stimulated progesterone production. At a constant dose of lutropin, inhibition was dependent on the concentration of beta-subunit. When concentration of the beta-subunit was kept constant at 5.0 microgram/ml and the concentration of lutropin was varied, the inhibition was maximum at the saturating concentration of the native hormone. The alpha-subunit of the human chorionic gonadotropin did not inhibit the response to lutropin. The lutropin/beta-subunit ratio required to produce an inhibition of response was much lower than that required to bring about an observable inhibition of binding.

The Fine Structure of Rat Granulosa Cell Cultures Correlated with Progestin Secretion

1973 ◽  
Vol 31 ◽  
pp. 552-553
Author(s):  
T. M. Crisp ◽  
F.R. Denys
Keyword(s):  
Fine Structure ◽  
Granulosa Cell ◽  
Cell Cultures ◽  
Single Injection ◽  
Surrounding Medium ◽  
Petri Dish ◽  
Female Rats ◽  
Vaginal Smear ◽  
Immature Female ◽  
Serum Gonadotropin

The purpose of this paper is to present observations on the fine structure of rat granulosa cell cultures grown in the presence of an adenohypophyseal explant and to correlate the morphology of these cells with progestin secretion. Twenty-six day old immature female rats were given a single injection of 5 IU pregnant mares serum gonadotropin (PMS) in order to obtain ovaries with large vesicular follicles. At 66 hrs. post-PMS administration (estrus indicated by vaginal smear cytology), the ovaries were removed and placed in a petri dish containing medium 199 and 100 U penicillin/streptomycin (P/S)/ml. Under a 20X magnification dissecting microscope, some 5-8 vesicular follicles/ovary were punctured and the granulosa cells were expressed into the surrounding medium. The cells were transferred to centrifuge tubes and spun down at 1000 rpm for 5 mins.

Follicle stimulating hormone versus pregnant mare serum gonadotropin for superovulation of dairy goats

1992 ◽  
Vol 8 (3) ◽  
pp. 217-224 ◽  
Author(s):  
R.J. Pendleton ◽  
C.R. Youngs ◽  
R.W. Rorie ◽  
S.H. Pool ◽  
M.A. Memon ◽  
...  
Keyword(s):  
Follicle Stimulating Hormone ◽  
Dairy Goats ◽  
Pregnant Mare Serum Gonadotropin ◽  
Serum Gonadotropin ◽  
Stimulating Hormone
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