Inhibition of aromatase activity and growth suppression by 4-methoxy-4-androstene-3,17-dione in an androgen sensitive human prostatic carcinoma cell line

1996 ◽  
Vol 101 (2) ◽  
pp. 143-148 ◽  
Author(s):  
Janice L. Block ◽  
Norman L. Block ◽  
Bal L. Lokeshwar
Keyword(s):  
Cell Line ◽  
Prostatic Carcinoma ◽  
Carcinoma Cell ◽  
Growth Suppression ◽  
Carcinoma Cell Line ◽  
Aromatase Activity

scholarly journals Does Bromelain-Cisplatin Combination Afford In-Vitro Synergistic Anticancer Effects on Human Prostatic Carcinoma Cell Line, PC3?

2020 ◽  
Vol 9 ◽  
pp. 1749
Author(s):  
Fatemeh Amini Chermahini ◽  
Elham Raeisi ◽  
Mathias Hossain Aazami ◽  
Abbas Mirzaei ◽  
Esfandiar Heidarian ◽  
...  
Keyword(s):  
Gene Expression ◽  
Cell Viability ◽  
Cell Line ◽  
Colony Formation ◽  
Prostatic Carcinoma ◽  
Carcinoma Cell ◽  
Quantitative Polymerase Chain Reaction ◽  
Chemotherapeutic Agents ◽  
Carcinoma Cell Line

Background: Bromelain enhances anticancer impacts to chemotherapeutic agents. The question as to whether bromelain does promote in-vitro cytotoxic and proapoptotic effects of cisplatin on human prostatic carcinoma PC3 cell line was investigated. Materials and Methods: PC3 (human prostatic carcinoma) cells were treated either single or in combination with bromelain and/or cisplatin. MTT, clonogenic assay, flow cytometry and real-time quantitative polymerase chain reaction were used to investigate cell viability, colony formation, proapoptotic potential and p53 gene expression, respectively. Results: Cisplatin (IC10) combined with bromelain (IC40) significantly affected PC3 cell viability, inhibited colony formation, as well increased p53 proapoptotic gene expression compared to cisplatin single treatment. Nevertheless, bromelain-cisplatin chemoherbal combination did not display any additive proapoptotic effect compared to single treatments. Conclusion: Bromelain-cisplatin chemoherbal combination demonstrated synergistic in-vitro anticancer effect on human prostatic carcinoma cell line, PC3, that drastically reduced required cisplatin dose. [GMJ.2020;9:e1749]

Effects of Hormones on Growth and ATP Content of a Human Prostatic Carcinoma Cell Line, LNCaP-r

1986 ◽  
Vol 135 (4) ◽  
pp. 882-883
Author(s):  
M. Hasenson ◽  
B. Hartley-Asp ◽  
C. Kihlfors ◽  
A. Lundin ◽  
J.-Å. Gustafsson ◽  
...  
Keyword(s):  
Cell Line ◽  
Prostatic Carcinoma ◽  
Carcinoma Cell ◽  
Carcinoma Cell Line ◽  
Atp Content

scholarly journals Forskolin up-regulates aromatase (CYP19) activity and gene transcripts in the human adrenocortical carcinoma cell line H295R

2004 ◽  
Vol 180 (1) ◽  
pp. 125-133 ◽  
Author(s):  
M Watanabe ◽  
S Nakajin
Keyword(s):  
Cell Line ◽  
Adrenocortical Carcinoma ◽  
Carcinoma Cell ◽  
Carcinoma Cell Line ◽  
Adrenal Tumors ◽  
Aromatase Activity ◽  
Gene Transcript ◽  
Aromatase Expression ◽  
Cyp19 Gene ◽  
Gene Transcripts

A number of conditions related to sex-reversal in boys and men and precocious puberty in girls are caused by estrogen-secreting adrenal tumors. In these tumors, cytochrome P450 aromatase (aromatase) that is encoded in the CYP19 gene is expressed at high levels. To investigate the molecular mechanism of aromatase expression in these adrenal tumors, we characterized the activity, gene transcript and genomic promoter region of aromatase in the human adrenocortical carcinoma cell line H295R. Aromatase activity and the transcript of the CYP19 gene were highly up-regulated by forskolin, but not by dexamethasone. The results from exon I-specific reverse transcriptase (RT)-PCR and the transfection of reporter constructs suggested that promoter I.3 and promoter II were activated in H295R. Deletion and mutation analysis suggested that cAMP response element-like sequence (CLS) and steroidogenic factor-1 (SF-1) motif, were critical for the activation of promoter II. The results of this work should provide the basis for the molecular analysis of aromatase expression in adrenocortical cells.

Inhibitory effects of vincristine, ifosfamide and peplomycin on hormone-independent human prostatic carcinoma cell line

1988 ◽  
Vol 35 (3) ◽  
pp. 141-148
Author(s):  
N. Moriyama-gonda ◽  
M. Mizutani ◽  
S. Ehara ◽  
T. Usui ◽  
T. Ishibe
Keyword(s):  
Cell Line ◽  
Prostatic Carcinoma ◽  
Carcinoma Cell ◽  
Carcinoma Cell Line ◽  
Inhibitory Effects

Establishment and Characterization of a New Human Prostatic Carcinoma Cell Line (DuPro-1)

1991 ◽  
Vol 146 (3) ◽  
pp. 915-919 ◽  
Author(s):  
Jeffrey R. Gingrich ◽  
J. Allan Tucker ◽  
Philip J. Walther ◽  
John W. Day ◽  
Susan H.M. Poulton ◽  
...  
Keyword(s):  
Cell Line ◽  
Prostatic Carcinoma ◽  
Carcinoma Cell ◽  
Carcinoma Cell Line
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