Presence of lipids in the secretory/excretory product from Toxocara canis

Introduction: Parasitic myositis is caused by some parasites such as T. gondii and T. canis. So, the aim of the study was to evaluate the prevalence T. gondii and T. canis in patients with myositis and healthy individuals. Methods: A total of 108 samples were randomly selected as the control (54 healthy individuals) and test (54 myositis patients) groups. IgG and IgM antibodies against T. gondii and IgG antibodies against T. canis were measured by the ELISA. The detection of chronic and acute toxoplasmosis was performed by the ELISA IgG avidity. The presence of T. gondii in blood was evaluated by the nested-PCR. Results: Of 108, 33 (30.6%) cases were detected positive for IgG against T. gondii that 19 (35.2%) and 14 (25.9%) were observed in myositis patients and healthy individuals, respectively (P=0.296). Of 19 positive cases, 12 (63.2%) and 7 (36.8%) cases were detected as chronic and acute toxoplasmosis, respectively, while, all positive cases in the control group had chronic toxoplasmosis (P=0.013). One (1.9%) sample was detected positive for anti- Toxoplasma gondii IgM and two (3.7%) samples were found positive for IgG against T. canis by the ELISA that these positive cases were observed only in myositis patients (P=1.000 P=0.495, respectively). B1 T. gondii gene was amplified in 12 (63.2%) and 1 (7.1%) in myositis patients and healthy subjects (P=0.001). Conclusions: Our findings showed that there was a relatively high prevalence of acute toxoplasmosis in myositis patients in comparison with the control subjects in southwest of Iran.

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A comparison of the protein constituents of the major body compartments of the dog roundworm, Toxocara canis

Veterinary Parasitology ◽

10.1016/j.vetpar.2007.08.049 ◽

2007 ◽

Vol 150 (1-2) ◽

pp. 111-115 ◽

Cited By ~ 1

Author(s):

Tiffany Sun ◽

Mary L. Bellosa ◽

Araceli Lucio-Forster ◽

Dean S. Ho ◽

Dwight D. Bowman

Keyword(s):

Toxocara Canis ◽

Body Compartments

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Human density, economic level and frequency of canine helminths in Buenos Aires

Journal of Helminthology ◽

10.1017/s0022149x21000055 ◽

2021 ◽

Vol 95 ◽

Author(s):

D. Rubel ◽

N. Flaibani

Keyword(s):

Linear Models ◽

Buenos Aires ◽

Toxocara Canis ◽

The Other ◽

Green Spaces ◽

Cross Sectional ◽

Independent Variables ◽

Economic Level ◽

Faecal Samples ◽

Human Density

Abstract The aim of this study was to explore through cross-sectional study the variation in the prevalence of parasitic helminths in canine faeces collected from green spaces of Buenos Aires according to the human density (HD) and economic level (EL) in the surroundings. HD and EL were considered as independent variables with three categories each. Twenty public squares (one hectare of surface) were randomly selected for each existing combination of the two independent variables. Ten random samples of fresh canine faeces were obtained in each square and analysed for helminths by the sedimentation and flotation techniques. The prevalence for each of the species was analysed using generalized linear models (GLM). The prevalence was modelled with a binomial error distribution and a logit link function. Helminth eggs were detected in 45 out of the 200 (22.5%) faecal samples collected and in 18 of the 20 green spaces sampled. The species observed were Ancylostoma caninum (13% of samples), Trichuris vulpis (8%) and Toxocara canis (4.5%). The GLM indicated that the prevalence of A. caninum in the slum areas (very high HD and very low EL) was higher than that in the other areas studied. However, the HD seemed to contribute more than the EL to the variations in the prevalence of A. caninum in faecal samples. The GLM showed no differences in the prevalence of the other parasite species for the different levels of the independent variables.

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Development of a low-cost copro-LAMP assay for simultaneous copro-detection of Toxocara canis and Toxocara cati

Parasitology ◽

10.1017/s0031182021000342 ◽

2021 ◽

pp. 1-8

Author(s):

Héctor Gabriel Avila ◽

Marikena Guadalupe Risso ◽

Paula Ruybal ◽

Silvia Analía Repetto ◽

Marcos Javier Butti ◽

...

Keyword(s):

Low Cost ◽

Lamp Assay ◽

Toxocara Canis ◽

Toxocara Cati

Abstract

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Infectivity and pathogenicity of 14-month-cultured embryonated eggs of Toxocara canis in mice

Veterinary Parasitology ◽

10.1016/s0304-4017(03)00046-3 ◽

2003 ◽

Vol 113 (2) ◽

pp. 145-155 ◽

Cited By ~ 35

Author(s):

Chia-Kwung Fan ◽

Yun-Ho Lin ◽

Wen-Yuan Du ◽

Kua-Eyre Su

Keyword(s):

Toxocara Canis

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Cross-reactions of sera from Toxascaris leonina and Ascaris suum infected mice with Toxocara canis, Toxascaris leonina and Ascaris suum antigens

International Journal for Parasitology ◽

10.1016/0020-7519(94)00187-s ◽

1995 ◽

Vol 25 (6) ◽

pp. 731-739 ◽

Cited By ~ 19

Author(s):

C. Cuéllar ◽

S. Fenoy ◽

J.L. Guillén

Keyword(s):

Ascaris Suum ◽

Toxocara Canis ◽

Cross Reactions ◽

Toxascaris Leonina

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Decrease in litter size of female mice with Toxocara canis

Transactions of the Royal Society of Tropical Medicine and Hygiene ◽

10.1016/0035-9203(90)90164-a ◽

1990 ◽

Vol 84 (5) ◽

pp. 724 ◽

Cited By ~ 6

Author(s):

Nobuaki Akao ◽

Robert S. Desowitz ◽

Kaoru Kondo

Keyword(s):

Litter Size ◽

Toxocara Canis ◽

Female Mice

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Seroepidemiological study and associated risk factors of Toxocara canis infection among preschool children in Osun State, Nigeria

Acta Tropica ◽

10.1016/j.actatropica.2017.05.030 ◽

2017 ◽

Vol 173 ◽

pp. 85-89 ◽

Cited By ~ 6

Author(s):

Oluyomi A. Sowemimo ◽

Yueh-Lun Lee ◽

Samuel O. Asaolu ◽

Ting-Wu Chuang ◽

Olaoluwa P. Akinwale ◽

...

Keyword(s):

Risk Factors ◽

Preschool Children ◽

Toxocara Canis ◽

Associated Risk Factors

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Standardization of dot-ELISA for the serological diagnosis of toxocariasis and comparision of the assay with ELISA

Revista do Instituto de Medicina Tropical de São Paulo ◽

10.1590/s0036-46651992000100010 ◽

1992 ◽

Vol 34 (1) ◽

pp. 55-60 ◽

Cited By ~ 34

Author(s):

Eide Dias Camargo ◽

Paulo Mutuko Nakamura ◽

Adelaide José Vaz ◽

Marcos Vinícius da Silva ◽

Pedro Paulo Chieffi ◽

...

Keyword(s):

Low Cost ◽

Clinical Signs ◽

Toxocara Canis ◽

Enzyme Linked Immunosorbent Assay ◽

Serum Samples ◽

Specific Antibodies ◽

Normal Individuals ◽

Before And After ◽

Dot Elisa ◽

Stability Time

The dot-enzyme-linked immunosorbent assay (dot-ELISA) was standardized using somatic (S) and excretory-secretory (ES) antigens of Toxocara-canis for the detection of specific antibodies in 22 serum samples from children aged 1 to 15 years, with clinical signs of toxocariasis. Fourteen serum samples from apparently normal individuals and 28 sera from patients with other pathologies were used as controls. All samples were used before and after absorption with Ascaris suum extract. When the results were evaluated in comparison with ELISA, the two tests were found to have similar sensitivity, but dot-ELISA was found to be more specific in the presence of the two antigens studied. Dot-ELISA proved to be effective for the diagnosis of human toxocariasis, presenting advantages in terms of yield, stability, time and ease of execution and low cost.

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Cardiorespiratory and gastrointestinal parasites of dogs in north-west Italy

Helminthologia ◽

10.1515/helmin-2016-0032 ◽

2016 ◽

Vol 53 (4) ◽

pp. 318-325 ◽

Cited By ~ 3

Author(s):

L. Guardone ◽

M. Magi ◽

M. C. Prati ◽

F. Macchioni

Keyword(s):

Intestinal Parasites ◽

Single Species ◽

Toxocara Canis ◽

Gastrointestinal Parasites ◽

Multiple Infections ◽

North West ◽

Sampling Programme ◽

Trichuris Vulpis ◽

Toxascaris Leonina ◽

Eucoleus Boehmi

SummaryThe present study was conducted to evaluate the prevalence of gastrointestinal and cardio-respiratory parasites in dogs in Liguria (northwest Italy) which is a region that has scarcely been studied. A total of 450 dogs (260 males and 190 females) were enrolled in the study from 2009 and 2013. All dogs lived in a rural or semi-rural environment in the provinces of Imperia (n = 352) or Savona (n = 98), Liguria. Coprological examinations showed that 197 dogs (43.8 %, CI 38.7-48.9 %) were infected by parasites. Specifically, 3.3 % of the dogs presented only cardio-respiratory species, 32.4 % only intestinal species, and 8.0 % presented a mixed infection.The most frequent intestinal parasites wereToxocara canis(20 %),Trichuris vulpis(17.8 %), Ancylostomatidae (12 %), Coccidia (2.7 %),Aonchotheca putorii(1.8 %) andToxascaris leonina(1.8 %). The cardio-respiratory species found wereEucoleus aerophilus(9.6 %),Eucoleus boehmi(1.6 %),Angiostrongylus vasorum(0.7 %), andCrenosoma vulpis(0.2 %). A total of 116 dogs (25.8 %) were parasitized by a single species, multiple infections were observed in 81 dogs (18.0 %) up to a six-order infection.This preliminary study highlighted that the prevalence of intestinal parasites in investigated area is high. Cardio respiratory parasites were detected in an area that has not been investigated before. The creation of a more extensive sampling programme of the area, on a provincial basis in order to build a more detailed map of prevalences for different species of dog parasites throughout Liguria, a more extensive sampling programme of the area needs to be created - ideally for each province.

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