Protein synthesis in plant mitochondria☆III. Characterization of mitochondria and the microsomal fractionm of the seedlings of vigna sinensis

1964 ◽  
Vol 93 (2) ◽  
pp. 304-310 ◽  
Author(s):  
H DAS ◽  
T MUKHERJEE
Keyword(s):  
Protein Synthesis ◽  
Plant Mitochondria ◽  
Vigna Sinensis

scholarly journals Organelle Genetics in Plants

2021 ◽  
Vol 22 (4) ◽  
pp. 2104
Author(s):  
Pedro Robles ◽  
Víctor Quesada
Keyword(s):  
Rna Editing ◽  
Posttranscriptional Regulation ◽  
Genome Engineering ◽  
Plant Mitochondria ◽  
Plastid Dna ◽  
Chloroplast Genomes ◽  
Wide Range ◽  
Organelle Genetics ◽  
Selection Of

Eleven published articles (4 reviews, 7 research papers) are collected in the Special Issue entitled “Organelle Genetics in Plants.” This selection of papers covers a wide range of topics related to chloroplasts and plant mitochondria research: (i) organellar gene expression (OGE) and, more specifically, chloroplast RNA editing in soybean, mitochondria RNA editing, and intron splicing in soybean during nodulation, as well as the study of the roles of transcriptional and posttranscriptional regulation of OGE in plant adaptation to environmental stress; (ii) analysis of the nuclear integrants of mitochondrial DNA (NUMTs) or plastid DNA (NUPTs); (iii) sequencing and characterization of mitochondrial and chloroplast genomes; (iv) recent advances in plastid genome engineering. Here we summarize the main findings of these works, which represent the latest research on the genetics, genomics, and biotechnology of chloroplasts and mitochondria.

scholarly journals Characterization of the ATP-dependent binding of wheat germ protein synthesis initiation factors eIF-(iso)4F and eIF-4A to mRNA.

1993 ◽  
Vol 268 (25) ◽  
pp. 18599-18603
Author(s):  
M.L. Balasta ◽  
S.E. Carberry ◽  
D.E. Friedland ◽  
R.A. Perez ◽  
D.J. Goss
Keyword(s):  
Protein Synthesis ◽  
Wheat Germ ◽  
Initiation Factors ◽  
Protein Synthesis Initiation

scholarly journals Protein Synthesis in Plant Mitochondria

1964 ◽  
Vol 239 (4) ◽  
pp. 1126-1133
Author(s):  
H.K. Das ◽  
S.K. Chatterjee ◽  
S.C. Roy
Keyword(s):  
Protein Synthesis ◽  
Plant Mitochondria

scholarly journals Characterization of the lectin from the bulbs of Eranthis hyemalis (winter aconite) as an inhibitor of protein synthesis.

1993 ◽  
Vol 268 (33) ◽  
pp. 25176-25183
Author(s):  
M A Kumar ◽  
D E Timm ◽  
K E Neet ◽  
W G Owen ◽  
W J Peumans ◽  
...  
Keyword(s):  
Protein Synthesis ◽  
Inhibitor Of Protein Synthesis

scholarly journals In Vitro Import of a Nuclearly Encoded tRNA into Mitochondria of Solanum tuberosum

2003 ◽  
Vol 23 (11) ◽  
pp. 4000-4012 ◽  
Author(s):  
Ludovic Delage ◽  
André Dietrich ◽  
Anne Cosset ◽  
Laurence Maréchal-Drouard
Keyword(s):  
Solanum Tuberosum ◽  
Higher Plants ◽  
Plant Mitochondria ◽  
Protein Translation ◽  
Trna Genes ◽  
Vitro Assay ◽  
Trna Import

ABSTRACT Some of the mitochondrial tRNAs of higher plants are nuclearly encoded and imported into mitochondria. The import of tRNAs encoded in the nucleus has been shown to be essential for proper protein translation within mitochondria of a variety of organisms. Here, we report the development of an in vitro assay for import of nuclearly encoded tRNAs into plant mitochondria. This in vitro system utilizes isolated mitochondria from Solanum tuberosum and synthetic tRNAs transcribed from cloned nuclear tRNA genes. Although incubation of radioactively labeled in vitro-transcribed tRNAAla, tRNAPhe, and tRNAMet-e with isolated potato mitochondria resulted in importation, as measured by nuclease protection, the amount of tRNA transcripts protected at saturation was at least five times higher for tRNAAla than for the two other tRNAs. This difference in in vitro saturation levels of import is consistent with the in vivo localization of these tRNAs, since cytosolic tRNAAla is naturally imported into potato mitochondria whereas tRNAPhe and tRNAMet-e are not. Characterization of in vitro tRNA import requirements indicates that mitochondrial tRNA import proceeds in the absence of any added cytosolic protein fraction, involves at least one protein component on the surface of mitochondria, and requires ATP-dependent step(s) and a membrane potential.

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