Isolation of malate dehydrogenase from cell walls of Nicotiana tabacum

Floral organs (sepals, petals, stamens, carpels) have formed de novo and directly from explants composed of 3 to 6 layers of epidermal and subepidermal cells; these organs originated from the subepidermal layer. The first symptoms of the resumption of mitotic activity included incorporation of tritiated thymidine at the level of the subepidermal layer followed by new cell walls observed after 24 h of culturing. On the average, organogenesis from cells derived from the subepidermal layer was completed after about 10 days.This work demonstrates that the epidermal tissue can form flower buds de novo and directly. [Translated by the journal]

Download Full-text

Using Viscoelastic Properties of the Woody Tissue from Tobacco Plants (Nicotiana tabacum) to Comment on the Molecular Structure of Cell Walls

Annals of Botany ◽

10.1006/anbo.1998.0619 ◽

1998 ◽

Vol 81 (6) ◽

pp. 729-734 ◽

Cited By ~ 13

Author(s):

D HEPWORTH

Keyword(s):

Molecular Structure ◽

Nicotiana Tabacum ◽

Viscoelastic Properties ◽

Cell Walls ◽

Woody Tissue ◽

Tobacco Plants

Download Full-text

A pectic polysaccharide from cell walls of tobacco (Nicotiana tabacum) mesophyll

Carbohydrate Research ◽

10.1016/0008-6215(86)84018-6 ◽

1986 ◽

Vol 158 ◽

pp. 205-216 ◽

Cited By ~ 17

Author(s):

Shigero Eda ◽

Katsuhiro Miyabe ◽

Yukio Akiyama ◽

Akio Ohnishi ◽

Kunio Katö

Keyword(s):

Nicotiana Tabacum ◽

Cell Walls ◽

Pectic Polysaccharide

Download Full-text

Changes in the water binding characteristics of the cell walls from transgenic Nicotiana tabacum leaves with enhanced levels of peroxidase activity

Physiologia Plantarum ◽

10.1111/j.1399-3054.2004.00429.x ◽

2004 ◽

Vol 122 (4) ◽

pp. 504-512 ◽

Cited By ~ 14

Author(s):

Jose A. Mercado ◽

Antonio J. Matas ◽

Antonio Heredia ◽

Victoriano Valpuesta ◽

Miguel A. Quesada

Keyword(s):

Nicotiana Tabacum ◽

Peroxidase Activity ◽

Cell Walls ◽

Water Binding ◽

Binding Characteristics ◽

Transgenic Nicotiana Tabacum

Download Full-text

A galactoglucomannan from cell walls of suspension-cultured tobacco (Nicotiana tabacum) cells

Carbohydrate Research ◽

10.1016/0008-6215(85)85158-2 ◽

1985 ◽

Vol 137 ◽

pp. 173-181 ◽

Cited By ~ 23

Author(s):

Shigeru Eda ◽

Yukio Akiyama ◽

Kunio Katō ◽

Atsushi Ishizu ◽

Junzo Nakano

Keyword(s):

Nicotiana Tabacum ◽

Cell Walls

Download Full-text

Ectodesmata as Revealed By Freeze-Etch Preparations of Plant Epidermal Cell Walls

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100072782 ◽

1973 ◽

Vol 31 ◽

pp. 468-469

Author(s):

N.C. Lyon ◽

W. C. Mueller

Keyword(s):

Electron Microscopy ◽

Acetic Acid ◽

Nitric Acid ◽

Oxalic Acid ◽

Light Microscopy ◽

Epidermal Cell ◽

Cell Walls ◽

Plant Viruses ◽

Plant Leaves ◽

Thin Sections

Schumacher and Halbsguth first demonstrated ectodesmata as pores or channels in the epidermal cell walls in haustoria of Cuscuta odorata L. by light microscopy in tissues fixed in a sublimate fixative (30% ethyl alcohol, 30 ml:glacial acetic acid, 10 ml: 65% nitric acid, 1 ml: 40% formaldehyde, 5 ml: oxalic acid, 2 g: mecuric chloride to saturation 2-3 g). Other workers have published electron micrographs of structures transversing the outer epidermal cell in thin sections of plant leaves that have been interpreted as ectodesmata. Such structures are evident following treatment with Hg++ or Ag+ salts and are only rarely observed by electron microscopy. If ectodesmata exist without such treatment, and are not artefacts, they would afford natural pathways of entry for applied foliar solutions and plant viruses.

Download Full-text

An ultrastructural study of vegetative incompatibility in plants

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100083047 ◽

1978 ◽

Vol 36 (2) ◽

pp. 436-437

Author(s):

Randy Moore

Keyword(s):

Ultrastructural Study ◽

Cell Walls ◽

Growth And Development ◽

Solanum Pennellii ◽

Initial Product ◽

Basic Aspect ◽

Tissue Interactions ◽

Graft Interface ◽

Antigen Antibody ◽

Standard Fixation

Cell and tissue interactions are a basic aspect of eukaryotic growth and development. While cell-to-cell interactions involving recognition and incompatibility have been studied extensively in animals, there is no known antigen-antibody reaction in plants and the recognition mechanisms operating in plant grafts have been virtually neglected.An ultrastructural study of the Sedum telephoides/Solanum pennellii graft was undertaken to define possible mechanisms of plant graft incompatibility. Grafts were surgically dissected from greenhouse grown plants at various times over 1-4 weeks and prepared for EM employing variations in the standard fixation and embedding procedure. Stock and scion adhere within 6 days after grafting. Following progressive cell senescence in both Sedum and Solanum, the graft interface appears as a band of 8-11 crushed cells after 2 weeks (Fig. 1, I). Trapped between the buckled cell walls are densely staining cytoplasmic remnants and residual starch grains, an initial product of wound reactions in plants.

Download Full-text

Comparison of Substructures Between Uniaxial and Biaxial Deformation in 1100-0 Aluminum

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100096989 ◽

1981 ◽

Vol 39 ◽

pp. 52-53

Author(s):

D. L. Rohr ◽

S. S. Hecker

Keyword(s):

Plastic Strain ◽

Cell Walls ◽

Room Temperature ◽

Mechanical Response ◽

Biaxial Tension ◽

Initial Deformation ◽

Uniaxial Deformation ◽

Biaxial Deformation ◽

Stress States ◽

Comprehensive Study

As part of a comprehensive study of microstructural and mechanical response of metals to uniaxial and biaxial deformations, the development of substructure in 1100 A1 has been studied over a range of plastic strain for two stress states.Specimens of 1100 aluminum annealed at 350 C were tested in uniaxial (UT) and balanced biaxial tension (BBT) at room temperature to different strain levels. The biaxial specimens were produced by the in-plane punch stretching technique. Areas of known strain levels were prepared for TEM by lapping followed by jet electropolishing. All specimens were examined in a JEOL 200B run at 150 and 200 kV within 24 to 36 hours after testing.The development of the substructure with deformation is shown in Fig. 1 for both stress states. Initial deformation produces dislocation tangles, which form cell walls by 10% uniaxial deformation, and start to recover to form subgrains by 25%. The results of several hundred measurements of cell/subgrain sizes by a linear intercept technique are presented in Table I.

Download Full-text