Micro-propagation of strawberry plants in vitro — Effect of growth regulators on incidence of multi-apex abnormality

1982 ◽  
Vol 16 (4) ◽  
pp. 331-341 ◽  
Author(s):  
H.M. Anderson ◽  
A.J. Abbott ◽  
Sally Wiltshire
2016 ◽  
Vol 8 (2) ◽  
pp. 535-540
Author(s):  
Priyadarshani P. Mohapatra ◽  
V.K. Batra ◽  
Subhash Kajla ◽  
Anil K. Poonia ◽  
N. Manoj Kumar

In the present investigation, experiment was conducted for in vitro micro-propagation with different concentration of growth regulators in different explants Sprouts and Shoot tips of potato cultivar Kufri Frysona. The maximum survival percentage (40) of sprouts and (100%) of shoot tips were obtained when the explants were surface sterilized with 0.2% bavistin & 0.4% streptocyclin (45minutes) and 0.1% mercuric chloride (60seconds). Sterilized explants were inoculated on MS basal supplemented with various growth regulators and established successfully. The maximum shoot induction (62.5±1.44%) in 11.3±0.33 days and (74.0 ± 2.13 %) in 10.0 ± 0.50 days were reported on medium PM1 (BAP 0.25 mg/l) in sprouts and shoot tip explants respectively. The sprouted explants were further sub-cultured on MS media supplemented with various growth regulator alone and in combination for in vitro multiplication. In Kufri Frysona (11.2) shoots were obtained on MS medium fortified with 0.25mg/l BAP + 0.01mg/l IAA on 42th day of subculture. In vitro rooting was observed on MS basal medium supplemented with 2.0 mg/l NAA in Kufri Frysona after 10 days. Rooted plantlets were successfully hardened in green house using different types of potting mixture and finally transferred to field. The protocol will be very useful for large-scale production of disease free planting material of potato (S. tuberosum) in future.


2020 ◽  
Vol 11 (1) ◽  
Author(s):  
Kothula Pravalika

Banana (Musa spp.) is a large herbaceous perennial monocotyledonous and monocarpic plant. It belongs to the family Musaceae. Banana is one of the popular berry fruit which is available to every common person. Banana crop is mostly cultivated through sword suckers, for ease production some in vitro propagation methods came into existence like tissue culture technology. Some of them are micro propagation, clonal propagation etc, For in vitro growth of the excited tissues, plant growth regulators have been considered as one of the most significant factor. Most commonly, auxins and cytokinins are used in the growth and development of the banana explants. The plant growth regulators are required in different concentrations along with Murashige and Skoog media for the best shooting and rooting of the explants.


2016 ◽  
Vol 3 (4) ◽  
pp. 326-331
Author(s):  
Md. Arifuzzaman ◽  
Sabiha Sultana ◽  
Md. Saddam Hossain ◽  
Saifullah Saifullah ◽  
Rifat Al-Naser ◽  
...  

2020 ◽  
Vol 11 (1) ◽  
Author(s):  
Katkam Priyanka

Banana is important fruit crop in horticulture where it propagates through suckers which takes immense time for production and with low multiplication rate, to overcome these type of situation some protocols have been made such as the micro propagation where the tissue culture of banana is applied with help of plant growth regulators such as auxins and cytokinins are used at different concentration to attain the definite good results till now many studies had been done in the tissue culture of banana. In the present study it was observed that the explants cultured in MS medium containing 4 mg/l BAP + 0.5 mg/l IAA had highest number of shoot buds and number of shoots. Similar result was studied by Muhammad et al. (2007) where the highest multiplication ratio was observed at 4 mg/l BAP along with 1 mg/l IAA. Habiba et al. (2002) and Ahmed et al. (2014) reported that 4 mg/l BAP in combination with 2 mg/l IAA shown remarkable results.


Planta Medica ◽  
2010 ◽  
Vol 76 (12) ◽  
Author(s):  
N Saowakon ◽  
P Chaichanasak ◽  
C Wanichanon ◽  
V Reutrakul ◽  
P Sobhon

Planta Medica ◽  
2011 ◽  
Vol 77 (12) ◽  
Author(s):  
N Saowakon ◽  
P Kueakhai ◽  
N Changklungmoa ◽  
N Lorsuwannarat ◽  
P Sobhon

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