The long-term effect of cold on the metabolism of winter wheat seedlings. I. growth and hydration of seedlings at in vitro conditions

The long-term effect of N and P fertilisation on the grain yield, yield components and quality parameters of winter wheat was studied for different sites in the National Long-term Fertilisation Trials in Hungary. This network of small-plot fertilisation experiments has been maintained since 1967 in nine different agro-ecological regions of the country. Increasing P and N doses had a considerable effect on the grain yield, yield components and quality parameters of winter wheat according to the results evaluated in the first 20-year average of the experiments. However, these effects differed depending on the agro-ecological conditions of the sites. Yield increases could be detected at lower fertiliser doses, and improvement in quality parameters at higher rates.

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Assessment of Cholesterol, Glycemia Control and Short- and Long-Term Antihypertensive Effects of Smooth Hound Viscera Peptides in High-Salt and Fructose Diet-Fed Wistar Rats

Marine Drugs ◽

10.3390/md17040194 ◽

2019 ◽

Vol 17 (4) ◽

pp. 194 ◽

Cited By ~ 3

Author(s):

Ola Abdelhedi ◽

Hana Khemakhem ◽

Rim Nasri ◽

Mourad Jridi ◽

Leticia Mora ◽

...

Keyword(s):

Protein Hydrolysate ◽

Biological Properties ◽

Term Effect ◽

High Salt ◽

Antihypertensive Activity ◽

Long Term Effect ◽

Peptide Fraction ◽

Short And Long Term

In this study, the antihypertensive activity of Purafect®-smooth hound viscera protein hydrolysate (VPH) and its peptide fraction with molecular weight (MW) below 1 kDa (VPH-I) was investigated. In addition, the lipase inhibitory activity, as well the anticoagulant potential, in vitro, were assessed. The antihypertensive effects of VPH and VPH-I were studied during 24 h (short-term effect) and 30 days (long-term effect) using high-salt (18% NaCl) and -fructose (10%) diet (HSFD)-induced hypertension. Data showed that, 4 h post-administration of VPH and VPH-I (200 mg/kg BW), the systolic blood pressure of rats was reduced by about 6 and 9 mmHg, respectively. These effects were similar to that obtained with Captopril (~9 mmHg at t = 4 h). On the other hand, exposing the rats to daily to HSFD, coupled to the administration of viscera peptides, was found to attenuate hypertension. In addition, the proteins’ treatments were able to correct lipid and glycemic disorders, by reducing the total cholesterol and triglyceride contents and resorting to the plasma glucose level, compared to the HSFD group. Overall, the present findings demonstrated the preventive effect of VPH-peptides from hypertension complications, as a result of their biological properties.

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Long Term Effect of Noradrenaline on Insulin Binding to Human Adipose Tissue “In Vitro”

Hormone and Metabolic Research ◽

10.1055/s-2007-1012416 ◽

1986 ◽

Vol 18 (10) ◽

pp. 718-719 ◽

Cited By ~ 2

Author(s):

M. Cigolini ◽

C. Zancanaro ◽

E. Cavallo ◽

D. Benati ◽

S. Ferrari ◽

...

Keyword(s):

Adipose Tissue ◽

Human Adipose Tissue ◽

Insulin Binding ◽

Term Effect ◽

Adipose Tissue In Vitro ◽

Long Term Effect

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Fludarabine Treated CLL Patients Showed an Increased Chemoresistance Ex-Vivo.

Blood ◽

10.1182/blood.v104.11.2791.2791 ◽

2004 ◽

Vol 104 (11) ◽

pp. 2791-2791

Author(s):

Timo Schinkothe ◽

Michael J. Hallek ◽

Peter Staib

Keyword(s):

Overall Survival ◽

Response Rates ◽

Term Effect ◽

Chemosensitivity Test ◽

Primary Therapy ◽

Secondary Resistance ◽

Long Term Effect ◽

Pre Treatment

Abstract Fludarabine is the most successful drug in patients with relapsed, progradient or refractory chronic lymphocytic leukemia (CLL). But also the use of fludarabine in primary therapy as an alternative to chlorambucil is in trial. Fludarabine induces higher response rates, but showed no improvement in overall survival. The reason for this clinical observation is unclear. In the present study we wanted to answer the question, if the induction of secondary resistance might be the cause for this contradiction. METHODS. Peripheral blood samples from 42 patients with B-CLL were tested in the in-vitro chemosensitivity-test DiSC-assay with 11 drugs. 10 patients had received no therapy before the time of carrying out the In vitro test. 23 patients were pre-treated with prednisone, 8 patient with fludarabine and 5 patients with vincristine. Most of the patients received more then one drug before testing. The chemosensitivity were determined using the LC90. RESULTS. The patients were divided into those who were pre-treated vs. not pre-treated with the fludarabine, prednisone or vincristine. The influences of pre-treatment to the sensitivity were analysed using the Mann-Whitney-U test for two independent groups. With fludarabine pre-treated patients had an significantly increased chemoresistance against fludarabine (2.4-fold; p=.004). The increase was also significant for bendamustin (4.4-fold), 2-CdA (3.4-fold), Gemcitabine (2.7-fold) and daunorubicin (1.5-fold). There was no significant influence observed onto the other drugs. Pre-treatment with vincristine or prednisone had no impact onto the chemosensitivity of any tested drug. INTERPRETATION AND CONCLUSIONS. The results of this study demonstrate the capability of fludarabine to increase the chemoresistance against various drugs in CLL. The use of fludarabine in primary therapy should be discussable. The fact that fludarabine induces higher response rates, without an improvement in overall survival, might be understandable if the fludarabine treatment induces a negative long-term effect. The widely increase of the chemoresistance is such a negative long-term effect. Based on the presented data, we postulate that fludarabine induces a so called secondary resistance by selecting leukemic cells with a lower chemosensitivity under treatment.

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Long-term effect of glucocorticoid on connective tissue of aorta and skin Morphological and biochemical studies of tissues from rabbits with intact and injured aortas

Acta Endocrinologica ◽

10.1530/acta.0.0950271 ◽

1980 ◽

Vol 95 (2) ◽

pp. 271-281 ◽

Cited By ~ 10

Author(s):

R. Manthorpe ◽

C. Garbarsch ◽

I. Lorenzen

Keyword(s):

Connective Tissue ◽

Amino Nitrogen ◽

Vascular Wall ◽

Term Effect ◽

Intact Skin ◽

Long Term Effect ◽

Biochemical Analyses

Abstract. The long-term effect of prednisolone — 0.6 mg/day for 63 days — upon mechanically induced inflammation and repair processes in vascular connective tissue was compared with that upon undamaged vascular wall and intact skin of rabbits. The investigations included histological examination of aorta as well as biochemical analyses of collagen and various glycosaminoglycan fractions, RNA, DNA and alpha-amino nitrogen. The metabolism of collagen was estimated by in vitro labelling with [14C]proline and the metabolism of glycosaminoglycans by in vivo labelling with [35S]O4. The radioactivity of [125I]albumin in the aorta and serum was also studied. The collagen, glycosaminoglycans, RNA, DNA and water of vascular connective tissue during inflammation and repair and of intact skin was found to be more sensitive to the action of prednisolone than the connective tissue of undamaged vascular wall. An increased degradation of newly synthesized collagen was observed in damaged aorta as well as in skin in which also the biosynthesis of collagen was inhibited. Prednisolone inhibited the biosynthesis of glycosaminoglycans and decreased the total amount of glycosaminoglycans and of nucleic acids in the damaged aortas and the skin. The [125I]albumin aorta-to-serum ratio was significantly increased in the damaged aorta. Prednisolone treatment decreased the ratio in injured aortas, but elevated the ratio in the undamaged vessels. Prednisolone inhibited intimal thickening of the injured aortas.

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