Objective: We aimed to investigate the free radical scavenging, antioxidant and hepatoprotective potential of M. concanensis Nimmo leaves.Methods: Free radical scavenging activity was evaluated by employing various accepted in vitro systems, such as 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS), hydroxyl (OH) and nitric oxide (NO) radical. Antioxidant potential of M. concanensis Nimmo extract was assessed against H2O2 in goat liver by determination of superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx), glutathione S-transferase (GST), glucose-6-phosphatase dehydrogenase (G-6-PDH), total reduced glutathione (GSH), vitamin C, vitamin E activity, and lipid peroxidation (LPO).Results: Results showed that the amount of plant extract of M. concanensis Nimmo required to scavenge 50% of the DPPH radicals was 401.80 μg/ml, ABTS radical was 353.14 μg/ml, OH radical was 433.71 μg/ml and NO radical was 371.24 μg/ml. Also, the pre-treatment of ethanolic extract of M. concanensis Nimmo leaves in goat liver showed a significant protection against H2O2 induced oxidative stress by retaining of antioxidants of SOD (4.76±0.25), CAT (26.81±0.25), GPx (6.41±0.34), GST (2.31±0.10), G-6-PDH (0.84±0.02), GSH (37.30±1.12), vitamin C (3.01±0.07), vitamin E (14.43±0.45) within normal range.Conclusion: In conclusion, promising free radical scavenging, the antioxidant activity of M. concanensis Nimmo leaves can be able to treat various diseases caused by free radicals.
In Vitro Evaluation of Free Radical Scavenging, Fe2+ and SNP-Induced Lipid Peroxidation (Rat Brain) Activities of Methanolic Extracts from Three (3) Northern Nigerian Plants Leaf
