Characterization of the 16S-23S rRNA intergenic spacer of Bartonella bacilliformis

Gene ◽  
1994 ◽  
Vol 143 (1) ◽  
pp. 149-150 ◽  
Author(s):  
Michael F. Minnick ◽  
Joan C. Strange ◽  
Kurt F. Williams
Keyword(s):  
Intergenic Spacer ◽  
23S Rrna ◽  
Bartonella Bacilliformis

Haemoparasites of common shrews (Sorex araneus) in Northwest England

Parasitology ◽  
2007 ◽  
Vol 134 (6) ◽  
pp. 819-826 ◽  
Author(s):  
D. P. BRAY ◽  
K. J. BOWN ◽  
P. STOCKLEY ◽  
J. L. HURST ◽  
M. BENNETT ◽  
...  
Keyword(s):  
Intergenic Spacer ◽  
Comparative Sequence Analysis ◽  
Sorex Araneus ◽  
Clethrionomys Glareolus ◽  
23S Rrna ◽  
Published Data ◽  
Rrna Sequence ◽  
16S Rrna Sequence ◽  
The Uk

SUMMARYThe presence of haemoparasites belonging to the taxaAnaplasma,BartonellaandTrypanosomawas determined among 76 common shrews (Sorex araneus) from Northwest England.Anaplasma phagocytophilumDNA was recovered from the blood of 1 shrew (1·3%), with the amplified 16S rRNA sequence identical to one previously reported from a bank vole (Clethrionomys glareolus).Trypanosomaspp. DNA was detected in 9 shrews (11·8%), the amplified 18S rDNA fragments being indistinguishable from one another, and distinct from previously published data. This represents the first report of trypanosome infection inS. araneusand suggests they are susceptible to an uncharacterizedTrypanosomaspecies. Blood from 11 shrews (14·5%) yieldedBartonellaspp., with characterization of isolates using comparative sequence analysis of partialgltAand 16S-23S rRNA intergenic spacer regions revealing 2 different genotypes. Phylogenetic inference from alignment of partialgltAsequences found that both UKS. araneustypes formed a well-supported cluster withBartonellasp. isolated fromS. araneusin Sweden. No significant effect of host age, sex, or year of collection was found on prevalence ofBartonellaor trypanosome infections. The results of this survey demonstrate that common shrews in the UK are susceptible to haemoparasitic infections, at prevalences similar to those reported from sympatric rodents.

scholarly journals Bartonella Infections in Cats and Cat Fleas in Lithuania

Pathogens ◽  
2021 ◽  
Vol 10 (9) ◽  
pp. 1209
Author(s):  
Miglė Razgūnaitė ◽  
Indrė Lipatova ◽  
Algimantas Paulauskas ◽  
Birutė Karvelienė ◽  
Vita Riškevičienė ◽  
...  
Keyword(s):  
Bartonella Henselae ◽  
Intergenic Spacer ◽  
Its Region ◽  
23S Rrna ◽  
Zoonotic Infections ◽  
Ctenocephalides Canis ◽  
Cat Fleas ◽  
Vector Borne ◽  
Pcr Targeting

Bartonella are vector-borne parasitic bacteria that cause zoonotic infections in humans. One of the most common infections is cat-scratch disease caused by Bartonella henselae and Bartonella clarridgeiae. Cats are the major reservoir for these two species of bacteria, while cat fleas are vectors for the transmission of infection agents among cats. The aim of the present study was to investigate the presence of Bartonella infections in stray and pet cats and in cat fleas in Lithuania. Blood samples were taken from 163 cats presented in pet clinics and animal shelters. A total of 102 fleas representing two species, Ctenocephalides felis and Ctenocephalides canis, were collected from 12 owned cats that live both outdoors and indoors. Bartonella DNA in samples was detected using a nested PCR targeting the 16S–23S rRNA intergenic spacer (ITS) region. Bartonella DNA was detected in 4.9% (8/163) of the cats and 29.4% (30/102) of the fleas. Sequence analysis of the ITS region showed that the cats and fleas were infected with B. henselae, B. clarridgeiae and Bartonella sp., closely related to B. schoenbuchensis. This study is the first report on the prevalence and molecular characterization of Bartonella spp. in cats and cat fleas in Lithuania.

scholarly journals Characterization of the 16S-23S rRNA Intergenic Spacer Regions among Strains of the Fusobacterium necrophorum Cluster

2002 ◽  
Vol 64 (3) ◽  
pp. 273-276 ◽  
Author(s):  
Jinghua JIN ◽  
Delong XU ◽  
Wonganun NARONGWANICHGARN ◽  
Yoshitaka GOTO ◽  
Takeshi HAGA ◽  
...  
Keyword(s):  
Intergenic Spacer ◽  
Fusobacterium Necrophorum ◽  
23S Rrna

scholarly journals Cultivation-Independent Characterization of Methylobacterium Populations in the Plant Phyllosphere by Automated Ribosomal Intergenic Spacer Analysis

2008 ◽  
Vol 74 (7) ◽  
pp. 2218-2228 ◽  
Author(s):  
Claudia Knief ◽  
Lisa Frances ◽  
Franck Cantet ◽  
Julia A. Vorholt
Keyword(s):  
Intergenic Spacer ◽  
23S Rrna ◽  
Rrna Gene ◽  
Length Variation ◽  
Ribosomal Intergenic Spacer ◽  
Pcr Products ◽  
23S Rrna Gene ◽  
Reverse Primer ◽  
Insight Into

ABSTRACT Bacteria of the genus Methylobacterium are widespread in the environment, but their ecological role in ecosystems, such as the plant phyllosphere, is not very well understood. To gain better insight into the distribution of different Methylobacterium species in diverse ecosystems, a rapid and specific cultivation-independent method for detection of these organisms and analysis of their community structure is needed. Therefore, 16S rRNA gene-targeted primers specific for this genus were designed and evaluated. These primers were used in PCR in combination with a reverse primer that binds to the tRNAAla gene, which is located upstream of the 23S rRNA gene in the 16S-23S intergenic spacer (IGS). PCR products that were of different lengths were obtained due to the length heterogeneity of the IGS of different Methylobacterium species. This length variation allowed generation of fingerprints of Methylobacterium communities in environmental samples by automated ribosomal intergenic spacer analysis. The Methylobacterium communities on leaves of different plant species in a natural field were compared using this method. The new method allows rapid comparisons of Methylobacterium communities and is thus a useful tool to study Methylobacterium communities in different ecosystems.

scholarly journals Molecular Characterization of Fluoroquinolone-Resistant Bartonella bacilliformis

Pathogens ◽  
2021 ◽  
Vol 10 (7) ◽  
pp. 876
Author(s):  
Giovanna Mendoza-Mujica ◽  
Diana Flores-León ◽  
Joaquim Ruiz
Keyword(s):  
Amino Acid ◽  
Molecular Characterization ◽  
Amino Acid Change ◽  
23S Rrna ◽  
Amino Acid Substitutions ◽  
Bartonella Bacilliformis ◽  
Chloramphenicol Resistance ◽  
Ciprofloxacin Resistance ◽  
Double Substitution

The presence of amino acid changes in GyrA, GyrB, ParC, ParE, and in a proposed chromosomal chloramphenicol acetyl transferase (CAT), as well as mutations at 23S rRNA, were established by PCR and sequencing in 38 B. bacilliformis clinical isolates from four different areas in Peru. Eighteen out of 24 (75%) isolates showing ciprofloxacin resistance for both disk-diffusion and e-test presented amino acid substitutions in GyrA (G89C, six isolates, A91V, 1 isolate) GyrB (S474F, 10 isolates) or both (GyrA D95N and GyrB S474F, one isolate). Two out of 14 susceptible isolates presented amino acid substitutions at GyrB (S474F) or a double substitution GyrA D95N and GyrB S474F. Of note, ciprofloxacin-resistant isolates were recovered in the four areas studied. No amino acid change was observed at ParC or ParE. Only one isolate showed chloramphenicol resistance, but no alteration was present in either 23S rRNA or CAT. B. bacilliformis resistant to quinolones are extended throughout Peru, with amino acid substitutions at GyrA or GyrB as the main, albeit not exclusive, cause. B. bacilliformis seems to have an apparent facility to develop mutations on GyrB outside the classical positions 91, 95 of GyrA and 85, 88 of ParC.

scholarly journals Molecular characterization of a ceftriaxone-resistant Neisseria gonorrhoeae strain found in Switzerland: a case report

2021 ◽  
Vol 20 (1) ◽  
Author(s):  
Konrad Egli ◽  
Anna Roditscheff ◽  
Ursula Flückiger ◽  
Martin Risch ◽  
Lorenz Risch ◽  
...  
Keyword(s):  
Neisseria Gonorrhoeae ◽  
23S Rrna ◽  
Rrna Gene ◽  
Limited Information ◽  
Specific Pcr ◽  
Appropriate Treatment ◽  
23S Rrna Gene ◽  
Allele Type ◽  
Ciprofloxacin Resistance

Abstract Background The resistance of Neisseria gonorrhoeae to ceftriaxone is unusual in Switzerland. The underlying genotype responsible for resistance is suspected to be novel. Generally, resistance in Neisseria gonorrhoeae (Ng) involves a comprehensive set of genes with many different mutations leading to resistance to different β-lactams and fluoroquinolones. Case presentation A patient had a positive result from specific PCR for Ng. We routinely culture all clinical specimens with a positive NG-PCR. In this particular case, we isolated a strain with resistance to ceftriaxone in Switzerland. A total of seven different genes (penA, ponA, porinB, mtr, gyrA, parC, 23S rRNA gene) in this strain were partially sequenced for comparison with phenotypic susceptibility testing. Interestingly, two different mutations in the porinB gene were observed, and data on this gene are limited. Information on the identified allele type of the penA gene is very limited as well. Three different mutations of parC and gyrA that correlate with ciprofloxacin resistance were found. The combination of ceftriaxone and ciprofloxacin resistance makes an appropriate treatment difficult to obtain due to multidrug resistance. Conclusion The combined results for all genes show the appearance of new mutations in central Europe either due to worldwide spread or the emergence of new genetic combinations of mutations.

Characterization of Fusarium spp. isolates by PCR-RFLP analysis of the intergenic spacer region of the rRNA gene (rDNA)

2006 ◽  
Vol 106 (3) ◽  
pp. 297-306 ◽  
Author(s):  
A. Llorens ◽  
M.J. Hinojo ◽  
R. Mateo ◽  
M.T. González-Jaén ◽  
F.M. Valle-Algarra ◽  
...  
Keyword(s):  
Intergenic Spacer ◽  
Rflp Analysis ◽  
Rrna Gene ◽  
Fusarium Spp ◽  
Intergenic Spacer Region ◽  
Pcr Rflp
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