scholarly journals Bartonella Infections in Cats and Cat Fleas in Lithuania

Pathogens ◽  
2021 ◽  
Vol 10 (9) ◽  
pp. 1209
Author(s):  
Miglė Razgūnaitė ◽  
Indrė Lipatova ◽  
Algimantas Paulauskas ◽  
Birutė Karvelienė ◽  
Vita Riškevičienė ◽  
...  
Keyword(s):  
Bartonella Henselae ◽  
Intergenic Spacer ◽  
Its Region ◽  
23S Rrna ◽  
Zoonotic Infections ◽  
Ctenocephalides Canis ◽  
Cat Fleas ◽  
Vector Borne ◽  
Pcr Targeting

Bartonella are vector-borne parasitic bacteria that cause zoonotic infections in humans. One of the most common infections is cat-scratch disease caused by Bartonella henselae and Bartonella clarridgeiae. Cats are the major reservoir for these two species of bacteria, while cat fleas are vectors for the transmission of infection agents among cats. The aim of the present study was to investigate the presence of Bartonella infections in stray and pet cats and in cat fleas in Lithuania. Blood samples were taken from 163 cats presented in pet clinics and animal shelters. A total of 102 fleas representing two species, Ctenocephalides felis and Ctenocephalides canis, were collected from 12 owned cats that live both outdoors and indoors. Bartonella DNA in samples was detected using a nested PCR targeting the 16S–23S rRNA intergenic spacer (ITS) region. Bartonella DNA was detected in 4.9% (8/163) of the cats and 29.4% (30/102) of the fleas. Sequence analysis of the ITS region showed that the cats and fleas were infected with B. henselae, B. clarridgeiae and Bartonella sp., closely related to B. schoenbuchensis. This study is the first report on the prevalence and molecular characterization of Bartonella spp. in cats and cat fleas in Lithuania.

scholarly journals Molecular Detection and Characterization of Borrelia garinii (Spirochaetales: Borreliaceae) in Ixodes nipponensis (Ixodida: Ixodidae) Parasitizing a Dog in Korea

Pathogens ◽  
2019 ◽  
Vol 8 (4) ◽  
pp. 289 ◽  
Author(s):  
Seung-Hun Lee ◽  
Youn-Kyoung Goo ◽  
Paul John L. Geraldino ◽  
Oh-Deog Kwon ◽  
Dongmi Kwak
Keyword(s):  
Molecular Detection ◽  
Single Gene ◽  
Protein A ◽  
Intergenic Spacer ◽  
Surface Protein ◽  
23S Rrna ◽  
Borrelia Garinii ◽  
Intergenic Spacer Region ◽  
Individual Level ◽  
Pcr Targeting

The present study aimed to detect and characterize Borrelia spp. in ticks attached to dogs in Korea. Overall, 562 ticks (276 pools) attached to dogs were collected and tested for Borrelia infection by PCR targeting the 5S-23S rRNA intergenic spacer region (rrf-rrl). One tick larva (pool level, 0.4%; individual level, 0.2%) was confirmed by sequencing Borrelia garinii, a zoonotic pathogen. For molecular characterization, the outer surface protein A (ospA) and flagellin genes were analyzed. Phylogenetic ospA analysis distinguished B. garinii from B. bavariensis, which has been recently identified as a novel Borrelia species. On the other hand, phylogenetic analysis showed that single gene analysis involving rrf-rrl or flagellin was not sufficient to differentiate B. garinii from B. bavariensis. In addition, the B. garinii-infected tick was identified as Ixodes nipponensis by sequencing according to mitochondrial 16S rRNA and the second transcribed spacer region. To our knowledge, this is the first study to report the molecular detection of B. garinii in I. nipponensis parasitizing a dog in Korea. Continuous monitoring of tick-borne pathogens in ticks attached to animals is required to avoid disease distribution and possible transmission to humans.

A putative transposase gene in the 16S–23S rRNA intergenic spacer region of Mycoplasma imitans

Microbiology ◽  
2004 ◽  
Vol 150 (4) ◽  
pp. 1023-1029 ◽  
Author(s):  
Ryô Harasawa ◽  
David G. Pitcher ◽  
Ana S. Ramírez ◽  
Janet M. Bradbury
Keyword(s):  
Relative Size ◽  
Intergenic Spacer ◽  
Its Region ◽  
23S Rrna ◽  
Mycoplasma Species ◽  
Insertion Element ◽  
Transposase Gene ◽  
Intergenic Spacer Region ◽  
Pcr Products ◽  
Type Strains

Examination of the nucleotide sequences of the 16S–23S intergenic transcribed spacer (ITS) region of Mycoplasma imitans and Mycoplasma gallisepticum identified a putative transposase gene located only in the ITS of M. imitans, which can be used as a genetic marker to distinguish these two species. The relative size of the PCR products of the ITS region allowed a clear distinction to be made between strains of M. imitans and M. gallisepticum, both of which could be readily discriminated from the type strains of all the other recognized avian Mycoplasma species. In addition, the putative transposase gene assigned in the ITS of M. imitans was shown to include a sequence homologous to that of the P75 gene of M. gallisepticum. This is believed to be the first description of an insertion element in the rRNA operon region of a mycoplasma species.

scholarly journals Molecular Detection of Bartonella Sp. in Wild Ruminants and Analysis of its Genetic Diversity on the Basis of 16S–23S Rrna Intergenic Spacer (ITS)

2012 ◽  
Vol 56 (1) ◽  
pp. 15-19
Author(s):  
Małgorzata Adamska
Keyword(s):  
Genetic Diversity ◽  
Cervus Elaphus ◽  
Phylogenetic Trees ◽  
Red Deer ◽  
Roe Deer ◽  
Intergenic Spacer ◽  
Capreolus Capreolus ◽  
Its Region ◽  
Its Sequences ◽  
23S Rrna

Abstract The aim of this study was to describe the state of infection of roe deer (Capreolus capreolus) and red deer (Cervus elaphus) by Bartonella sp. in North-Western Poland through PCR detection of Bartonella 16S-23S rRNA ITS region in isolates of animal tissues, and also to describe the genetic diversity of detected Bartonella species based on molecular analysis of ITS. The multiple alignment analysis of ITS sequences was carried out, and homology matrices and phylogenetic trees were constructed. The DNA of Bartonella sp. was detected in tissues of 45.6% (36/79) C. capreolus and of 50% (15/30) C. elaphus. Products of two different sizes were detected: 317 bp, characteristic for B. schoenbuchensis, and 198 bp, characteristic for B. bovis. The obtained results suggest that roe and red deer are potential reservoirs of Bartonella sp. Most of the analysed ITS sequences was not specific for one host species. In constructed phylogenetic trees, sequences obtained from roe and red deer clustered together. These results suggest a lack of host specificity of most detected B. schoenbuchensis and B. bovis intraspecies strains

Characterization of the 16S-23S rRNA intergenic spacer of Bartonella bacilliformis

Gene ◽  
1994 ◽  
Vol 143 (1) ◽  
pp. 149-150 ◽  
Author(s):  
Michael F. Minnick ◽  
Joan C. Strange ◽  
Kurt F. Williams
Keyword(s):  
Intergenic Spacer ◽  
23S Rrna ◽  
Bartonella Bacilliformis

scholarly journals Molecular survey ofBartonella henselaeandBartonella clarridgeiaein pet cats across Japan by species-specific nested-PCR

2017 ◽  
Vol 145 (13) ◽  
pp. 2694-2700 ◽  
Author(s):  
S. SATO ◽  
H. KABEYA ◽  
A. NEGISHI ◽  
H. TSUJIMOTO ◽  
K. NISHIGAKI ◽  
...  
Keyword(s):  
Nested Pcr ◽  
Bartonella Henselae ◽  
23S Rrna ◽  
Cat Scratch Disease ◽  
Internal Transcribed Spacer Region ◽  
Nested Polymerase Chain Reaction ◽  
Polymerase Chain ◽  
Species Specific ◽  
Pcr Targeting ◽  
Eastern Japan

SUMMARYCats are known to be the main reservoir forBartonella henselaeandBartonella clarridgeiae, which are the agents of ‘cat-scratch disease’ in humans. In the present study, we investigated the prevalence of the twoBartonellaspecies on 1754 cat bloods collected from all prefectures in Japan during 2007–2008 by a nested-polymerase chain reaction (PCR) targeting the 16S–23S rRNA internal transcribed spacer region. Overall,BartonellaDNA was detected in 4·6% (80/1754) of the cats examined. The nested-PCR showed that 48·8% (39/80) of the positive cats were infected withB. henselaemono-infection, 33·8% (27/80) withB. clarridgeiaemono-infection and 17·5% (14/80) were infected with both species. The prevalence (5·9%; 65/1103) ofBartonellainfection in the western part of Japan was significantly higher than that (2·3%; 15/651) of eastern Japan (P< 0·001). Statistical analysis of the cats examined suggested a significant association betweenBartonellainfection and FeLV infection (OR = 1·9; 95% CI = 1·1–3·4), but not with FIV infection (OR = 1·6; 95% CI = 1·0–2·6).

Haemoparasites of common shrews (Sorex araneus) in Northwest England

Parasitology ◽  
2007 ◽  
Vol 134 (6) ◽  
pp. 819-826 ◽  
Author(s):  
D. P. BRAY ◽  
K. J. BOWN ◽  
P. STOCKLEY ◽  
J. L. HURST ◽  
M. BENNETT ◽  
...  
Keyword(s):  
Intergenic Spacer ◽  
Comparative Sequence Analysis ◽  
Sorex Araneus ◽  
Clethrionomys Glareolus ◽  
23S Rrna ◽  
Published Data ◽  
Rrna Sequence ◽  
16S Rrna Sequence ◽  
The Uk

SUMMARYThe presence of haemoparasites belonging to the taxaAnaplasma,BartonellaandTrypanosomawas determined among 76 common shrews (Sorex araneus) from Northwest England.Anaplasma phagocytophilumDNA was recovered from the blood of 1 shrew (1·3%), with the amplified 16S rRNA sequence identical to one previously reported from a bank vole (Clethrionomys glareolus).Trypanosomaspp. DNA was detected in 9 shrews (11·8%), the amplified 18S rDNA fragments being indistinguishable from one another, and distinct from previously published data. This represents the first report of trypanosome infection inS. araneusand suggests they are susceptible to an uncharacterizedTrypanosomaspecies. Blood from 11 shrews (14·5%) yieldedBartonellaspp., with characterization of isolates using comparative sequence analysis of partialgltAand 16S-23S rRNA intergenic spacer regions revealing 2 different genotypes. Phylogenetic inference from alignment of partialgltAsequences found that both UKS. araneustypes formed a well-supported cluster withBartonellasp. isolated fromS. araneusin Sweden. No significant effect of host age, sex, or year of collection was found on prevalence ofBartonellaor trypanosome infections. The results of this survey demonstrate that common shrews in the UK are susceptible to haemoparasitic infections, at prevalences similar to those reported from sympatric rodents.

scholarly journals Molecular Identification of Borrelia spp. from Ticks in Pastures nearby Livestock Farms in Korea

Insects ◽  
2021 ◽  
Vol 12 (11) ◽  
pp. 1011
Author(s):  
Haeseung Lee ◽  
Seung-Hun Lee ◽  
SungShik Shin ◽  
Dongmi Kwak
Keyword(s):  
Tick Species ◽  
Pathogenic Bacteria ◽  
Intergenic Spacer ◽  
23S Rrna ◽  
Haemaphysalis Longicornis ◽  
Intergenic Spacer Region ◽  
Close Relationship ◽  
Haemaphysalis Flava ◽  
Ixodes Ticks ◽  
Pcr Targeting

Ticks are vectors that spread pathogenic bacteria, viruses, and protozoa. As the number of ticks increases due to climate change, the importance of the study of tick-borne pathogens has also increased. This study was conducted to investigate the distribution of the major tick species causing Lyme borreliosis and regional differences in the prevalence of Borrelia spp. by tick species. Borrelia infection was confirmed not only in Ixodes ticks, which are the major vectors of Borrelia spp., but also in Haemaphysalis and Amblyomma ticks. PCR targeting the 5S-23S rRNA intergenic spacer region (rrf-rrl) was performed to confirm Borrelia positivity. A total of 6102 ticks (736 pools) were tested, and the proportion was Haemaphysalis longicornis nymphs and adults at 69.2%, Haemaphysalis flava nymphs and adults at 13.9%, Haemaphysalis spp. larva at 14.3%, Ixodes nipponensis at 0.8%, and Amblyomma testudinarium at 1.9%. Ixodes nipponensis showed the highest minimum infection rate (MIR: 34.00; 17 pools/50 ticks) for Borrelia spp., followed by A. testudinarium (MIR: 0.88), and H. longicornis (MIR: 0.05). In particular, to our knowledge Borrelia infection was first confirmed in A. testudinarium in Korea. As a result of phylogenetic analysis, all sequences were grouped with Borreliaafzelii isolates and showed a close relationship with high identity. Considering that B. afzelii causes infectious zoonotic diseases, continuous monitoring and attention are needed, although it has a low prevalence in this study.

Molecular Differentiation of Renibacterium salmoninarum Isolates from Worldwide Locations

1999 ◽  
Vol 65 (3) ◽  
pp. 961-968 ◽  
Author(s):  
Thomas H. Grayson ◽  
Lynne F. Cooper ◽  
Franck A. Atienzar ◽  
Mark R. Knowles ◽  
Martyn L. Gilpin
Keyword(s):  
Rapd Analysis ◽  
Intergenic Spacer ◽  
Its Region ◽  
Single Copy ◽  
Rrna Genes ◽  
23S Rrna ◽  
Trna Genes ◽  
Renibacterium Salmoninarum ◽  
Pathogen Dna ◽  
Obligate Pathogen

ABSTRACT Renibacterium salmoninarum is a genospecies that is an obligate pathogen of salmonid fish and is capable of intracellular survival. Conventional typing systems have failed to differentiate isolates of R. salmoninarum. We used two methods to assess the extent of molecular variation which was present in isolates from different geographic locations. In one analysis we investigated possible polymorphisms in a specific region of the genome, the intergenic spacer (ITS) region between the 16S and 23S rRNA genes. In the other analysis we analyzed differences throughout the genome by using randomly amplified polymorphic DNA (RAPD). We amplified the spacer region of 74 isolates by using PCR and performed a DNA sequence analysis with 14 geographically distinct samples. The results showed that the 16S-23S ribosomal DNA spacer region of R. salmoninarum is highly conserved and suggested that only a single copy of the rRNA operon is present in this slowly growing pathogen. DNA sequencing of the spacer region showed that it was the same length in all 14 isolates examined, and the same nucleotide sequence, sequevar 1, was obtained for 11 of these isolates. Two other sequevars were found. No tRNA genes were found. We found that RAPD analysis allows reproducible differentiation between isolates of R. salmoninarum obtained from different hosts and different geographic regions. By using RAPD analysis it was possible to differentiate between isolates with identical ITS sequences.

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