Haemoparasites of common shrews (Sorex araneus) in Northwest England

Parasitology ◽  
2007 ◽  
Vol 134 (6) ◽  
pp. 819-826 ◽  
Author(s):  
D. P. BRAY ◽  
K. J. BOWN ◽  
P. STOCKLEY ◽  
J. L. HURST ◽  
M. BENNETT ◽  
...  
Keyword(s):  
Intergenic Spacer ◽  
Comparative Sequence Analysis ◽  
Sorex Araneus ◽  
Clethrionomys Glareolus ◽  
23S Rrna ◽  
Published Data ◽  
Rrna Sequence ◽  
16S Rrna Sequence ◽  
The Uk

SUMMARYThe presence of haemoparasites belonging to the taxaAnaplasma,BartonellaandTrypanosomawas determined among 76 common shrews (Sorex araneus) from Northwest England.Anaplasma phagocytophilumDNA was recovered from the blood of 1 shrew (1·3%), with the amplified 16S rRNA sequence identical to one previously reported from a bank vole (Clethrionomys glareolus).Trypanosomaspp. DNA was detected in 9 shrews (11·8%), the amplified 18S rDNA fragments being indistinguishable from one another, and distinct from previously published data. This represents the first report of trypanosome infection inS. araneusand suggests they are susceptible to an uncharacterizedTrypanosomaspecies. Blood from 11 shrews (14·5%) yieldedBartonellaspp., with characterization of isolates using comparative sequence analysis of partialgltAand 16S-23S rRNA intergenic spacer regions revealing 2 different genotypes. Phylogenetic inference from alignment of partialgltAsequences found that both UKS. araneustypes formed a well-supported cluster withBartonellasp. isolated fromS. araneusin Sweden. No significant effect of host age, sex, or year of collection was found on prevalence ofBartonellaor trypanosome infections. The results of this survey demonstrate that common shrews in the UK are susceptible to haemoparasitic infections, at prevalences similar to those reported from sympatric rodents.

Characterization of the 16S-23S rRNA intergenic spacer of Bartonella bacilliformis

Gene ◽  
1994 ◽  
Vol 143 (1) ◽  
pp. 149-150 ◽  
Author(s):  
Michael F. Minnick ◽  
Joan C. Strange ◽  
Kurt F. Williams
Keyword(s):  
Intergenic Spacer ◽  
23S Rrna ◽  
Bartonella Bacilliformis

scholarly journals Characterization Physiology and Molecular Bacteria Symbiont- Entomopathogenic Nematodes based of Gene Sequences Encoding the 16S rRNA District of Bromo Probolinggo

2017 ◽  
Vol 18 (1) ◽  
pp. 39
Author(s):  
Bagus Setiawan ◽  
Didik Sulistyanto ◽  
Kartika Senjarini
Keyword(s):  
16S Rrna ◽  
Entomopathogenic Nematodes ◽  
Phenotypic Characterization ◽  
Bacterial Symbionts ◽  
Rrna Sequence ◽  
16S Rrna Sequence ◽  
Gene Encoding ◽  
Bacillus Strain ◽  
Rrna Sequences

This study aims to identify entomopathogenic nematodes symbiotic bacteria phenotypically and based on the gene encoding 16S rRNA sequences. Bacterial symbionts of entomopathogenic nematodes, obtained from isolates from the area Wonokerto (WN01) and isolates Sukapura (SP01), Bromo, Probolinggo, two symbiont bacteria was found in entomopathogenic nematodes Steinernema sp. The method used in this study are: the isolation of entomopathogenic nematodes Steinernema sp. and bacterial symbionts conventionally for the identification of phenotypically, after the characterization of bacterial isolates, the isolation of genomic DNA, 16S rRNA PCR, DNA purification and DNA sequence analysis. The results based on phenotypic characterization showed that isolates WN01 and SP01, yellowish white, gram positive, negative bioluminenscene, catalase positive, can not hydrolyze urea, and also can not produce H2S. The results of the gene encoding 16S rRNA sequence can be deduced WN01 isolates have in common with the bacteria Bacillus strain toyonensis BCT 7112, while the SP01 isolates have in common with the bacteria Bacillus strain cereus ATCC 14 579.

scholarly journals Characterization of Yellow Pigmented Bacteria Associated with Gracilaria sp.

2020 ◽  
Vol 2 (2) ◽  
Author(s):  
Arina Tri Lunggani ◽  
Susianna Purwantisari ◽  
Siti Nur Jannah
Keyword(s):  
Room Temperature ◽  
Research Result ◽  
Growth Media ◽  
Bacterial Isolation ◽  
Rrna Sequence ◽  
16S Rrna Sequence ◽  
Kinship Analysis ◽  
16S Rrna Sequence Analysis ◽  
Pigmented Bacteria

Research on the kinship analysis of endophytic bacterial  isolated from Gracillaria sp has been carried out. The presence of bacteria associated with Gracilaria sp. has enabled the use of these bacteria as a source of new bioactive compounds, such as biopigments. The research aims to isolated bacteria from Gracilaria sp., screened their symbiont bacteria that could potentially produce pigments. Sampling Gracilaria sp. conducted in the waters of the Island of  Karimunjawa, Jepara. Furthermore, bacterial isolation was carried out, screening for pigment-producing bacteria and 16S rRNA sequence analysis. Research result showed that the symbiont bacteria isolate TK 373 produced consistent pigments after several regenerations, in several types of growth media incubated at room temperature. The results of 16S rDNA identification showed that the TK 373 isolate had the closest relationship with  Pseudoalteromonas sp. with  98.72 % homology.

Screening, Identification and Characterization of a Cyanoethylated Wastepaper-Degrading Bacteria

2013 ◽  
Vol 316-317 ◽  
pp. 349-353
Author(s):  
Chun Hong Zhang ◽  
Dong Dong Xiao ◽  
Pei Shi Sun ◽  
Ping Zou ◽  
Qiu Ling Chen
Keyword(s):  
Inoculum Size ◽  
Optimum Conditions ◽  
Rrna Sequence ◽  
16S Rrna Sequence ◽  
16S Rdna Sequence ◽  
Degrading Bacteria ◽  
Identification And Characterization ◽  
Humus Soil ◽  
Biochemical Features

A cyanoethylated wastepaper-degrading bacterial strain S11 was screened from the surface humus soil in the forest of Yunnan University. The bacterium was identified through analysis of morphological, biological and biochemical features and 16S rRNA sequence. S11 enjoyed above 99% homology with Ochrobactrum sp.CRRI29’s 16S rDNA sequence, therefore the baterium belonged to Ochrobactrum sp. The research on degradation features of S11 revealed that S11 bacterium could effectively degrade cyanoethylated wastepaper under 35°C, pH 11.0, 25% inoculum size and 50g/L substrate concentration; under the optimum conditions, the degradation rate of cyanoethylated wastepaper was 46.83% after degradation by S11 bacterium for 192 hours.

The helminth parasites of some small mammal communities. I. The parasites and their hosts

Parasitology ◽  
1964 ◽  
Vol 54 (1) ◽  
pp. 145-154 ◽  
Author(s):  
G. I. Sharpe
Keyword(s):  
Small Mammals ◽  
Small Mammal ◽  
Host Species ◽  
Sorex Araneus ◽  
Clethrionomys Glareolus ◽  
Published Data ◽  
Microtus Agrestis ◽  
Helminth Parasites ◽  
Small Mammal Communities ◽  
Mammal Communities

1. A method of trapping small mammals has been described and Apodemus sylvaticus, Clethrionomys glareolus, Microtus agrestis and Sorex araneus were captured. The method of trapping has been discussed.2. A survey of the endoparasites found in the four host species has been produced. The two more common hosts and four of their parasites have been chosen for more detailed study.3. The infection levels of the parasites in the two hosts have been calculated and compared with other published data.This work was carried out during the tenure of a Department of Scientific and Industrial Research Studentship. My thanks are due to Dr H. D. Crofton for his very helpful supervision and criticism and to Professor J. E. Harris, F.R.S., in whose department this work was done, for the facilities provided.

scholarly journals Isolation, purification and characterization of mannan degrading enzyme from a new isolate Acinetobacter baummanni

2021 ◽  
Vol 924 (1) ◽  
pp. 012078
Author(s):  
D Muzaki ◽  
E Zubaidah ◽  
S Santoso ◽  
A Sutrisno
Keyword(s):  
Enzyme Activity ◽  
Guar Gum ◽  
Relative Activity ◽  
Purification And Characterization ◽  
Rrna Sequence ◽  
16S Rrna Sequence ◽  
Ammonium Sulphate Precipitation ◽  
Optimum Ph ◽  
16S Rrna Sequence Analysis

Abstract A mannan-degrading microbe was isolated from rotting porang tubers (Amorphophallus muelleri Blume). Molecular identification using 16S-rRNA sequence analysis revealed that the isolate showed 99.67% similarity with Acinetobacter baummanni. A crude enzyme from ammonium sulphate precipitation was used for preliminary characterization. The characterization results showed that the enzyme activity is optimum at 45 °C, and stable at 35-50 °C, while the optimum pH is 7, and stable at pH 5-7. The substrate with the highest relative activity was found in guar gum which was 137.512%. The enzyme activity was inhibited by Ca, Na, K ions, and increased by Mn2+ ions.

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