Semi-automated solid-phase extraction procedure for drug screening in biological fluids using the ASPEC system in combination with Clean Screen DAU columns

1993 ◽  
Vol 613 (2) ◽  
pp. 289-294 ◽  
Author(s):  
Xiao-Hua Chen ◽  
Jan-Piet Franke ◽  
Kees Ensing ◽  
Jaap Wijsbeek ◽  
Rokus A. de Zeeuw
Keyword(s):  
Solid Phase Extraction ◽  
Drug Screening ◽  
Solid Phase ◽  
Biological Fluids ◽  
Extraction Procedure ◽  
Phase Extraction ◽  
Solid Phase Extraction Procedure ◽  
Automated Solid Phase Extraction

scholarly journals Uniform solid-phase extraction procedure for toxicological drug screening in serum and urine by HPLC with photodiode-array detection

1997 ◽  
Vol 43 (2) ◽  
pp. 312-325 ◽  
Author(s):  
Chi-Kong Lai ◽  
Ting Lee ◽  
Kam-Ming Au ◽  
Albert Yan-Wo Chan
Keyword(s):  
Solid Phase Extraction ◽  
Drug Screening ◽  
Solid Phase ◽  
Extraction Procedure ◽  
Phase Extraction ◽  
Urine Metabolites ◽  
Ultraviolet Spectra ◽  
Solid Phase Extraction Procedure ◽  
Array Detection ◽  
Serum And Urine

Abstract In this HPLC–diode-array detection method for toxicological drug screening, a mixed-mode solid-phase extraction procedure is optimized for isolation of a broad range of drugs from serum and urine. Basic, neutral, and weakly acidic drugs are uniformly recovered. The extract from the solid-phase cartridge is readily injected to a reversed-phase HPLC column for separation by gradient elution. Unknown drugs and metabolites in urine and serum samples from acute drug poisoning cases are rapidly identified by matching their retention times and ultraviolet spectra with hundreds of reference compounds in the library. Urine metabolites of common toxicants from various medications and drugs of abuse are recorded, with their changes of retention times and ultraviolet spectra as related to their metabolic transformations. Glucuronide conjugates of common benzodiazepines, tricyclic antidepressants, and beta-blockers are examined directly without chemical or enzymatic hydrolysis. The system is reliable for diverse clinical investigations of drug overdoses, drug-induced psychoses, and substance abuse.

An alternative to trial and error methodology in solid phase extraction: an original automated solid phase extraction procedure for analysing PAHs and PAH-derivatives in soot

RSC Advances ◽  
2014 ◽  
Vol 4 (63) ◽  
pp. 33636-33644 ◽  
Author(s):  
Auréa Andrade-Eiroa ◽  
Roya Shahla ◽  
Manolis N. Romanías ◽  
Philippe Dagaut
Keyword(s):  
Solid Phase Extraction ◽  
Solid Phase ◽  
Extraction Procedure ◽  
Phase Extraction ◽  
Trial And Error ◽  
Hydrophilic Interaction ◽  
Reverse Phase Hplc ◽  
Solid Phase Extraction Procedure ◽  
Phase Liquid ◽  
Automated Solid Phase Extraction

This paper introduces the generalization of reverse-phase HPLC fundamentals to normal-phase liquid chromatography, hydrophilic interaction chromatography (HILIC) and Automated Solid Phase Extraction (A-SPE).

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