Molecular size distribution of specific elements in petroleum crudes and 650 F + residua by size exclusion chromatography with inductively coupled plasma spectrometry detection

1985 ◽  
Vol 40 (1-2) ◽  
pp. 389-396 ◽  
Author(s):  
D.W. Hausler
Keyword(s):  
Size Distribution ◽  
Size Exclusion Chromatography ◽  
Inductively Coupled Plasma ◽  
Molecular Size ◽  
Size Exclusion ◽  
Plasma Spectrometry ◽  
Inductively Coupled ◽  
Inductively Coupled Plasma Spectrometry ◽  
Exclusion Chromatography ◽  
Molecular Size Distribution

scholarly journals A High-Throughput Size Exclusion Chromatography Method to Determine the Molecular Size Distribution of Meningococcal Polysaccharide Vaccine

2016 ◽  
Vol 2016 ◽  
pp. 1-7 ◽  
Author(s):  
Imran Khan ◽  
K. M. Taufiqur Rahman ◽  
S. M. Saad Us Siraj ◽  
Mahbubul Karim ◽  
Abdul Muktadir ◽  
...  
Keyword(s):  
Distribution Coefficient ◽  
Size Distribution ◽  
Size Exclusion Chromatography ◽  
Molecular Size ◽  
Polysaccharide Vaccine ◽  
Size Exclusion ◽  
Chromatography Method ◽  
Exclusion Chromatography ◽  
Calculated Distribution ◽  
Molecular Size Distribution

Molecular size distribution of meningococcal polysaccharide vaccine is a readily identifiable parameter that directly correlates with the immunogenicity. In this paper, we report a size exclusion chromatography method to determine the molecular size distribution and distribution coefficient value of meningococcal polysaccharide serogroups A, C, W, and Y in meningococcal polysaccharide (ACWY) vaccines. The analyses were performed on a XK16/70 column packed with sepharose CL-4B with six different batches of Ingovax® ACWY, a meningococcal polysaccharide vaccine produced by Incepta Vaccine Ltd., Bangladesh. A quantitative rocket immunoelectrophoresis assay was employed to determine the polysaccharide contents of each serogroup. The calculated distribution coefficient values of serogroups A, C, W, and Y were found to be0.26±0.16,0.21±0.11,0.21±0.11, and0.14±0.12, respectively, and met the requirements of British Pharmacopeia. The method was proved to be robust for determining the distribution coefficient values which is an obligatory requirement for vaccine lot release.

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