MOLECULAR SIZE DISTRIBUTION OF HUMIC SUBSTANCES: A COMPARISON BETWEEN SIZE EXCLUSION CHROMATOGRAPHY AND CAPILLARY ELECTROPHORESIS

Molecular size distribution of meningococcal polysaccharide vaccine is a readily identifiable parameter that directly correlates with the immunogenicity. In this paper, we report a size exclusion chromatography method to determine the molecular size distribution and distribution coefficient value of meningococcal polysaccharide serogroups A, C, W, and Y in meningococcal polysaccharide (ACWY) vaccines. The analyses were performed on a XK16/70 column packed with sepharose CL-4B with six different batches of Ingovax® ACWY, a meningococcal polysaccharide vaccine produced by Incepta Vaccine Ltd., Bangladesh. A quantitative rocket immunoelectrophoresis assay was employed to determine the polysaccharide contents of each serogroup. The calculated distribution coefficient values of serogroups A, C, W, and Y were found to be0.26±0.16,0.21±0.11,0.21±0.11, and0.14±0.12, respectively, and met the requirements of British Pharmacopeia. The method was proved to be robust for determining the distribution coefficient values which is an obligatory requirement for vaccine lot release.

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Rapid Size-Exclusion Chromatography Analysis of Molecular Size Distribution for Wheat Endosperm Protein

Cereal Chemistry ◽

10.1094/cchem.2000.77.4.451 ◽

2000 ◽

Vol 77 (4) ◽

pp. 451-453 ◽

Cited By ~ 38

Author(s):

O. R. Larroque ◽

F. Bekes

Keyword(s):

Size Distribution ◽

Size Exclusion Chromatography ◽

Molecular Size ◽

Size Exclusion ◽

Wheat Endosperm ◽

Chromatography Analysis ◽

Endosperm Protein ◽

Exclusion Chromatography ◽

Molecular Size Distribution

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Molecular-size-distribution-dependent aggregation of humic substances by Na(I), Ag(I), Ca(II), and Eu(III)

Colloids and Surfaces A Physicochemical and Engineering Aspects ◽

10.1016/j.colsurfa.2013.05.030 ◽

2013 ◽

Vol 434 ◽

pp. 9-15 ◽

Cited By ~ 11

Author(s):

Shuji Tamamura ◽

Ryutaro Ohashi ◽

Seiya Nagao ◽

Masayoshi Yamamoto ◽

Motohiro Mizuno

Keyword(s):

Humic Substances ◽

Size Distribution ◽

Molecular Size ◽

Molecular Size Distribution

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Multidetectors for Analysis of the Composition of Copolymers as a Function of Molecular Size Distribution by Steric Exclusion Chromatography

Rubber Chemistry and Technology ◽

10.5254/1.3542914 ◽

1973 ◽

Vol 46 (2) ◽

pp. 449-463 ◽

Cited By ~ 8

Author(s):

D. J. Harmon ◽

V. L. Folt

Keyword(s):

Liquid Chromatography ◽

Size Distribution ◽

Oil Content ◽

Molecular Size ◽

The Other ◽

Additional Information ◽

Steric Exclusion ◽

Gel Permeation ◽

Exclusion Chromatography ◽

Molecular Size Distribution

Abstract Analysis of molecular size distribution of polymers by steric exclusion liquid chromatography (GPC) is well known. Problems exist, however. These problems involve copolymers and polymer blends. The objectives of the research were to develop methods of analyzing comonomer distribution in copolymers, to study the breakdown of one polymer independent of another in a polymer blend, and to obtain any additional information as might be available. The separations were performed on a Waters Model 200 Gel Permeation Chromatograph. Detectors employed were a Waters R-4 differential refractometer, a Wilks Miran-1 infrared analyzer, and a Beckman Model 144 UV photometer. Examples are given of analysis of average styrene, styrene distribution, and oil content of oil extended SBR. The data is compared with that obtained by other methods. In general the agreement is good. The ability to examine one polymer of a blend independent of the other is also demonstrated. Since elastomers are frequently used as blends, this becomes very important to such studies as milling and extrusion behavior.

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