Impact of urine matrix and isolation procedure on retention behaviour of basic drugs in thin-layer chromatography

1988 ◽  
Vol 6 (4) ◽  
pp. 415-420 ◽  
Author(s):  
J.P. Franke ◽  
R.A. De Zeeuw ◽  
J. Wijsbeek
Keyword(s):  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Isolation Procedure ◽  
Basic Drugs ◽  
Retention Behaviour ◽  
Layer Chromatography ◽  
Urine Matrix

Single-pH Extraction Procedure for Detecting Drugs of Abuse

1974 ◽  
Vol 20 (2) ◽  
pp. 309-311 ◽  
Author(s):  
Ramon E Stoner ◽  
Connie Parker
Keyword(s):  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Drugs Of Abuse ◽  
Clinical Laboratory ◽  
Extraction Procedure ◽  
Organic Salt ◽  
Basic Drugs ◽  
Laboratory Service ◽  
The Individual ◽  
Layer Chromatography

Abstract A method is presented for determining certain drugs of abuse in a single extract of urine. The urine sample is adjusted to pH 6.0 with a buffer containing bromcresol purple. Basic drugs, such as amphetamines and narcotics, form an organic salt with the ionized bromcresol purple, which is extractable with a mixture of chloroform and 2-propanol (3:1 by volume). At pH 6.0, weak acids such as barbiturates and neutral drugs such as glutethimide are also soluble in this solvent. Consequently, the major classes of drugs are extracted simultaneously. The extract is then concentrated and the individual drugs are determined by thin-layer chromatography in a solvent that will separate bromcresol purple from the drugs. Additional Keyphrases: drug screening #{ 149} toxicology #{ 149} thin-layer chromatography #{ 149} screening procedure Clinical Laboratory Service, West Side VA Hosp., 820 S. Damen Ave., Chicago, Ill. 60612.

THE ISOLATION OF PROGESTERONE FROM HUMAN PREGNANCY URINE

1967 ◽  
Vol 56 (2) ◽  
pp. 272-278
Author(s):  
A. A. A. Ismail ◽  
R. A. Harkness
Keyword(s):  
Liquid Chromatography ◽  
Thin Layer ◽  
Physical Properties ◽  
Thin Layer Chromatography ◽  
Isolation Procedure ◽  
Gas Liquid Chromatography ◽  
Chromatographic Peak ◽  
Extractable Fraction ◽  
Pregnancy Urine ◽  
Layer Chromatography

ABSTRACT Progesterone was isolated from the »freely extractable« fraction of approximately 50 litres of pregnancy urine. Chemical and physical properties showed that the compound isolated was indeed progesterone. The isolation procedure involved a modified form of the Girard reaction in which a »conjugated ketone« fraction containing progesterone was separated from both the non-ketonic components and the major bulk of the saturated ketones. This was followed by paper and thin-layer chromatography. The final separation was achieved using preparative gas-liquid chromatography. It was estimated from the gas-chromatographic peak heights that there was no more than 250 μg of progesterone in the pooled urine residues.

scholarly journals THIN-LAYER CHROMATOGRAPHY ON REVERSED PHASE IN THE CHARACTERIZATION OF RETENTION BEHAVIOUR, LIPOPHILICITY, AND PHARMACOKINETICS OF CYANOACETAMIDE DERIVATIVES

2020 ◽  
Vol 65 (1) ◽  
pp. 4654-4660
Author(s):  
Suzana Apostolov ◽  
Gyöngyi Vastag ◽  
Borko Matijević ◽  
Tatjana Đaković-Sekulić ◽  
Aleksandar Marinković
Keyword(s):  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Reversed Phase ◽  
Retention Behaviour ◽  
Layer Chromatography
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