Changes in the Polyphenolic Profile and Antioxidant Activity of Wheat Bread after Incorporating Quinoa Flour

Quinoa is a trend and a promising functional food ingredient. Following previous research into the impact of incorporating quinoa flour on the polyphenol content and antioxidant activity of bread, this study aimed to bridge an existing gap about the qualitative and quantitative polyphenolic profiles of such bread. The UPLC-MS/MS analysis showed that quinoa bread, made with 25% quinoa flour of a black variety, presented more compounds than refined-wheat bread, and levels were remarkably higher in many cases. Consequently, the quinoa bread presented clearly improved polyphenolic content than the wheat bread (12.8-fold higher considering the sum of extractable and hydrolyzable polyphenols), as supported by greater antioxidant activity (around 3-fold). The predominant compounds in the extractable fraction of quinoa bread were p-hydroxybenzoic acid and quercetin (50- and 64-fold higher than in wheat bread, respectively) and rutin (not detected in wheat bread), while ferulic and sinapic acids were the most abundant compounds in the hydrolyzable fraction (7.6- and 13-fold higher than in wheat bread, respectively). The bread-making impact was estimated, and a different behavior for phenolic acids and flavonoids was observed. Extractable phenolic acids were the compounds that decreased the most; only 2 of 12 compounds were enhanced (p-hydroxybenozoic and rosmarinic acid with increments of 64% and 435%, respectively). Flavonoids were generally less affected, and their concentrations considerably rose after the bread-making process (7 of the 13 compounds were enhanced in the extractable fraction) with especially noticeably increases in some cases; e.g., apigenin (876%), kaempferol (1304%), luteolin (580%) and quercetin (4762%). Increments in some extractable flavonoids might be explained as a consequence of the release of the corresponding hydrolyzable forms. The present study provides new information on the suitability of quinoa-containing bread as a suitable vehicle to enhance polyphenols intake and, hence, the antioxidant activity in daily diets.

Age-old transformation of oil hydrocarbons in polluted Grey-Brown Soil in the Absheron Peninsula

Oil production, transportation and refining are a source of the extensive environmental pollution. The study of oil pollutants allows the identification of their behavior pattern necessary in order to elaborate efficient decontamination technologies. The Absheron oil field is one of the most long-term exploited deposit in the world. Today this area is a unique place to investigate the long-lasting natural oil transformation processes. Grey-Brown Soils contaminated with crude oil 100 and 40 years ago and currently were investigated. Hexane-extractable fraction of oil compounds was examined by chemical methods, gas chromatography and mass spectrometry. In the process of natural degradation, molecular weight of oil hydrocarbons and degree of oxidation significantly increased, while the contents of carbon and hydrogen slightly decreased with time. The results obtained have shown the accumulation of high-molecular weight aromatic molecules with high degree of unsaturation in the upper layer of the soil contaminated 100 years ago. These aromatic structures exhibit the great resistance to decomposition and may be carcinogenic, thus posing a risk to human health. The contamination with oil resulted in altering soil properties. More pronounced change was observed in the soil carbon, including the soil contaminated 100 years ago. Over time after contamination, the soil alkalinity enhanced, whereas soil pH did not change. Our study evidences that oil pollutants continue to be present and the soil properties have not recovered even 100 years after pollution. Reclamation of these areas requires the application of special technologies.

Phytochemical Profile and Antioxidant Properties of Bee-Collected Artichoke (Cynara scolymus) Pollen

The current study intended to determine, for the first time, phenolic and fatty acid profile, antioxidant and certain nutritional properties of monofloral bee-collected artichoke (Cynara scolymus) pollen. Based on UHPLC-DAD MS-MS analysis the main phenolics in extractable fraction were different flavonol glycosides (in particular Isorhamnetin-3-O-glucoside, 49.2 mg/kg of dry weight) while ferulic acid was the predominant phenolic compound (39.4 mg/kg of dry weight) in the alkaline hydrolyzable fraction. Among fatty acids (FAs), results of GC-FID analysis revealed prevalence of unsaturated FAs with cis-5,8,11,14,17-eicosapentaenoic acid (EPA) and oleic acid as the main ones- 28.4% and 24.9%, respectively. Based on the FA composition, nutritional analysis proved that artichoke bee-collected pollen had balanced ω-6 and ω-3 FAs content. To determine the antioxidant properties of pollen, five different assays were applied. It was proved that bioactive compounds in artichoke pollen possessed significant ability to quench DPPH radical as well as ABTS radical cation. In addition, in vitro phosphomolybdenum assay confirmed that artichoke pollen is an excellent source of different antioxidants. Pollen extracts exhibited moderate ferric reducing power as well as low ferrous chelating ability. Some further antioxidant studies (preferably in vivo) should be performed to confirm the observed results.

Nitrogen Fertilization Influences the Quantity, Composition, and Tissue Association of Foliar Phenolics in Strawberries

Unlike quantitative changes, the compositional changes of plant phenolics and changes in their tissue association as influenced by the nutrient supply are less well understood. We evaluated the quantity, composition, and tissue association of phenolics in leaves of two Fragaria ananassa cultivars in response to different levels of nitrogen (N) fertilization using global metabolomic approaches. Influence of N supply on phenolic content in both cultivars was similar, but the magnitude of this response was compound specific. Ellagitannins, the most abundant class of phenolic oligomers, were less responsive to the applied N treatments, whereas proanthocyanidins, the less abundant class of phenolic oligomers, exhibited higher fold change. Within mono-phenolics, the hydroxycinnamates were more abundant but showed lower fold change than the hydroxybenzoates. Among flavonoids, the hydroxylated flavonols showed higher abundances than the flavones, with a preferential accumulation of dihydroxylated flavonol at lower N levels. Furthermore, glycosylated flavonols were higher than the acylated forms. The extractable fraction of phenolics was more influenced by the N treatment than the fiber-bound fraction. The extensive compositional modification of phenolics and a greater response of non-bound fractions in response to N rates highlight the potential to use precise management of N supply as an effective strategy to enhance the bioactive compounds in crops.

Speciation of organosulfur compounds in carbonaceous chondrites

Despite broad application of different analytical techniques for studies on organic matter of chondrite meteorites, information about composition and structure of individual compounds is still very limited due to extreme molecular diversity of extraterrestrial organic matter. Here we present the first application of isotopic exchange assisted Fourier transform ion cyclotron resonance mass spectrometry (FTICR MS) for analysis of alkali extractable fraction of insoluble organic matter (IOM) of the Murchison and Allende meteorites. This allowed us to determine the individual S-containing ions with different types of sulfur atoms in IOM. Thiols, thiophenes, sulfoxides, sulfonyls and sulfonates were identified in both samples but with different proportions, which contribution corroborated with the hydrothermal and thermal history of the meteorites. The results were supported by XPS and thermogravimetric analysis coupled to FTICR MS. The latter was applied for the first time for analysis of chondritic IOM. To emphasize the peculiar extraterrestrial origin of IOM we have compared it with coal kerogen, which is characterized by the comparable complexity of molecular composition but its aromatic nature and low oxygen content can be ascribed almost exclusively to degradation of biomacromolecules.

Effect of pyrolysis temperature on sulfur content, extractable fraction and release of sulfate in corn straw biochar

Low pyrolysis temperature is optimal for biochar to release sulfate and the release kinetics fitted well with a pseudo-second-order model.

Nanopraticle Ferriferous Hydrosol as an Advanced Reagent for Waste Water Treatment

A new procedure for electroplating waste water treatment using a waste iron scrap by-product for nanoparticle ferriferous hydrosol (FFH) preparation is presented. FFH which contains Fe(II) and Fe(III) was employed for neutralization and heavy metal removal from electroplating wastewater as well as for decontamination of concomitant pollutants such as phosphates, organic compounds, residual oils, dyes and detergents. It is possible due to the simultaneous operation of several different mechanisms: sorption, coagulation, reduction, fertilization and etc. The method is suitable for the purposes of waste water treatment and disposal in compliance with environmental laws and is implemented in some East and West European countries. The results were proved in accredited laboratories in various countries. According to the composition of its water extractable fraction, remaining waste sludge could be safely deposited in urban waste repositories or used as a raw material for production of various technically useful products such as ceramics, pigment, etc. Treated water may be reused in technological processes.