Single-pH Extraction Procedure for Detecting Drugs of Abuse

1974 ◽  
Vol 20 (2) ◽  
pp. 309-311 ◽  
Author(s):  
Ramon E Stoner ◽  
Connie Parker
Keyword(s):  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Drugs Of Abuse ◽  
Clinical Laboratory ◽  
Extraction Procedure ◽  
Organic Salt ◽  
Basic Drugs ◽  
Laboratory Service ◽  
The Individual ◽  
Layer Chromatography

Abstract A method is presented for determining certain drugs of abuse in a single extract of urine. The urine sample is adjusted to pH 6.0 with a buffer containing bromcresol purple. Basic drugs, such as amphetamines and narcotics, form an organic salt with the ionized bromcresol purple, which is extractable with a mixture of chloroform and 2-propanol (3:1 by volume). At pH 6.0, weak acids such as barbiturates and neutral drugs such as glutethimide are also soluble in this solvent. Consequently, the major classes of drugs are extracted simultaneously. The extract is then concentrated and the individual drugs are determined by thin-layer chromatography in a solvent that will separate bromcresol purple from the drugs. Additional Keyphrases: drug screening #{ 149} toxicology #{ 149} thin-layer chromatography #{ 149} screening procedure Clinical Laboratory Service, West Side VA Hosp., 820 S. Damen Ave., Chicago, Ill. 60612.

An Evaluation of the Measurement of Drugs of Abuse by Commercial and In-House Horizontal Thin Layer Chromatography

1993 ◽  
Vol 30 (2) ◽  
pp. 163-168 ◽  
Author(s):  
K Wolff ◽  
M J Sanderson ◽  
A W M Hay ◽  
I Barnes
Keyword(s):  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Drug Addiction ◽  
Drugs Of Abuse ◽  
Routine Screening ◽  
Suitable Method ◽  
Screening Service ◽  
Laboratory Methods ◽  
Opioid Drugs ◽  
Layer Chromatography

We have compared an in-house horizontal thin layer chromatography (TLC) method with a commercial TLC screening kit (Toxi-Lab) to find the most suitable method for screening urine for opioid drugs. The in-house TLC procedure is cheaper and more efficient than the commercial TLC method and is ideal as a confirmatory method when used in conjunction with other established laboratory methods. The in-house TLC method is useful for one-off requests and is able to differentiate between small quantities (0·1–0·5 mg/L) of the different, commonly abused opioids and their metabolites, which means that it is well suited for use at a local Drug Addiction Unit as a routine screening service.

A METHOD FOR THE ESTIMATION OF INDIVIDUAL URINARY CORTICOSTEROIDS, PREGNANEDIOL, PREGNANETRIOL AND PREGNANETRIOLONE FOLLOWING THEIR SEPARATION BY THIN-LAYER CHROMATOGRAPHY

1969 ◽  
Vol 61 (1) ◽  
pp. 1-16 ◽  
Author(s):  
Z. Kraiem ◽  
B. Lunenfeld ◽  
J. Skalka
Keyword(s):  
Enzymatic Hydrolysis ◽  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Sulphuric Acid ◽  
Urine Sample ◽  
Blue Tetrazolium ◽  
Acid Reaction ◽  
Colorimetric Estimation ◽  
The Individual ◽  
Layer Chromatography

ABSTRACT A method is described for estimating urinary pregnanediol, pregnanetriol, pregnanetriolone and the following individual corticosteroids: tetrahydrocortisol (THF), allotetrahydrocortisol (allo-THF), tetrahydrocortisone (THE), cortisol (F), tetrahydro-11-deoxycortisol (THS), tetrahydrocorticosterone (THB), allotetrahydrocorticosterone (allo-THB), tetrahydro-11-dehydrocorticosterone (THA), tetrahydro-11-deoxycorticosterone (THDOC). The main steps of the method are: enzymatic hydrolysis, extraction, fractionation by thin-layer chromatography, and colorimetric estimation using the sulphuric acid reaction for pregnanediol, pregnanetriol and pregnanetriolone, and the blue tetrazolium reaction for the individual corticosteroids. The reliability criteria (specificity, accuracy, precision and sensitivity) of the method have been assessed. The advantage of the method lies in its practicability since, unlike other methods: a) the same urine sample (50 ml) is submitted, for the estimation of the twelve C21 steroids, to the same procedure until fractionation, and b) thin-layer chromatography alone is used for the latter purpose.

Urinary Homogentisic Acid: Determination by Thin-Layer Chromatography

1973 ◽  
Vol 19 (5) ◽  
pp. 459-462 ◽  
Author(s):  
Jerome M Feldman ◽  
June Bowman
Keyword(s):  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Clinical Laboratory ◽  
Homogentisic Acid ◽  
Qualitative And Quantitative ◽  
Color Development ◽  
Layer Chromatography ◽  
Phenol Reagent ◽  
Acid Determination

Abstract A new method is described for qualitative and quantitative determination of urinary homogentisic acid. The method involves extraction of urine with ethyl acetate, thin-layer chromatography of the extract on Silica Gel G, elution of the homogentisic acid into water, and color development with Folin’s phenol reagent. Absorbance is maximum at 750 nm and linear to a concentration of at least 5 mg of homogentisic acid per milliliter of urine. The method is highly specific for homogentisic acid; added gentisic acid, 3,4-dihydroxyphenylacetic acid, ascorbic acid, or L-3,4 dihydroxyphenylalanine do not interfere. The coefficient of variation "in-run" is 5.3%, "between-run" 6.8%. Using this method we have demonstrated a marked variation in day-to-day homogentisic acid excretion in a patient with alcaptonuria. This method, which offers some advantages over existing techniques, should be suitable for use in a clinical laboratory.

Analysis of Trisulfapyrimidine Preparations by Thin Layer Chromatography

1972 ◽  
Vol 55 (1) ◽  
pp. 194-196
Author(s):  
Charlotte A Brunner
Keyword(s):  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Collaborative Study ◽  
Colorimetric Measurement ◽  
Intact Sample ◽  
The Individual ◽  
Layer Chromatography

Abstract A method for the analysis of trisulfapyrimidine preparations is described. The total sulfonamide content is measured on the intact sample, and the ratio of the components is determined by colorimetric measurement of the individual compounds after separation by thin layer chromatography. A collaborative study will be performed.

Use of Fluorescamine ("Fluram") to Detect Amphetamine in Urine by Thin-Layer Chromatography

1974 ◽  
Vol 20 (2) ◽  
pp. 272-274 ◽  
Author(s):  
Bernard Klein ◽  
Joan E Sheehan ◽  
E Grunberg
Keyword(s):  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Silica Gel ◽  
Drugs Of Abuse ◽  
Thin Layer Plate ◽  
Spray Reagent ◽  
Layer Chromatography

Abstract A "Fluram" (fluorescamine; 4-phenylspiro[furan-2(3H)-1'-phthalan]-3,3'-dione) spray reagent will detect as little as 250 ng of amphetamine in an extract of amphetamine-containing urine, after it has been separated by thin-layer chromatography. The fluorescence is stable for about 20 h and can be renewed by respraying. Fluram spray does not interfere with reagent sprays used to detect other drugs of abuse. Amphetamine on the thin-layer plate can be measured by extracting the fluorescent area of silica gel and measuring the fluorescence in a fluorimeter. Recovery of 1.0 µg and 2.0 µg of amphetamine averaged 69% and 67%, respectively.

Dual-bed thin-layer chromatography for the separation and detection of drugs of abuse in urine

1973 ◽  
Vol 80 (2) ◽  
pp. 275-278 ◽  
Author(s):  
J.R. Broich ◽  
S. Goldner ◽  
G. Gourdet ◽  
S. Andryauskas ◽  
C.J. Umberger ◽  
...  
Keyword(s):  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Drugs Of Abuse ◽  
Layer Chromatography ◽  
Dual Bed

scholarly journals The Study of Ranitidine Interference With Morphine Detection Test by Thin-Layer Chromatography

2018 ◽  
Vol 6 (1) ◽  
pp. 21-23
Author(s):  
Amir Miri ◽  
Amir Karami ◽  
Fourogh Nadi ◽  
Fatemeh Zeraati
Keyword(s):  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Drugs Of Abuse ◽  
False Negative ◽  
Urine Samples ◽  
Urine Drug Screen ◽  
Test Results ◽  
Two Samples ◽  
Before And After ◽  
Layer Chromatography

Background: Drug abuse is a global and critical problem. One of the most frequent practices done in order to detect the drugs of abuse is Urine Drug Screen. However, for changing the drug test results, adulterants and urine substitutes are being designed. As the referring people’s background has shown, ranitidine is one of the interfering drugs in morphine detection test. Therefore, in the present study, the interference of ranitidine in morphine detection test will be studied. Methods: Ten healthy volunteers who had not used any kind of drug for 72 hours before the test were recruited into the study. First, 2 doses of ranitidine (150 and 300 mg) were administered to the subjects orally and 100-mL urine samples were collected from them before and after taking ranitidine. The second urine sample was collected at 6-8 am. Ten micrograms morphine was added to both urine samples of each individual. The urine samples were tested using thin-layer chromatography (TLC) technique. The experiment was repeated after 1 week using ranitidine 300 mg. Results: The TLC test was carried out on 40 urine samples. Twenty samples were tested before and, the rest, after ranitidine consumption. The TLC test results were positive before ranitidine consumption but negative for 18 samples and positive for two samples after taking ranitidine. Conclusion: Ranitidine may change the urine morphine screening test results via TLC method and induce a false negative result.

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