Experimental urinary tract infection in rabbits by a group D Streptococcus sp.: A study of serum immunoglobulin classes by enzyme-linked immunosorbent assay

Objective: To assess the frequency of proteus mirabilis infections in patients of rheumatoid arthritis. Study Design: Cross-sectional study. Place and Duration of Study: Combined Military Hospital, Rawalpindi Pakistan, from Jul 2018 to Jan 2019. Methodology: A total of 100 cases of rheumatoid arthritis with urinary tract infection were enrolled. Freshly voided urine samples were obtained and sent to the pathology lab. The serotypes of proteus mirabilis were evaluated by enzyme-linked immunosorbent assay (ELISA) and immunoblotting and presence of proteus mirabilis was noted. Results: A total of 100 cases of rheumatoid arthritis with urinary tract infection were recruited. The mean age of patients was 61.22 ± 10.18 years. There were total 67 (67%) females and 33 (33%) were males. About 79 (79%) patients had sedentary lifestyle while 21 (21%) had active lifestyle. There were 41 (41%) patients had positive urine culture with proteus mirabilis while 59 (59%) had other organism involved in urine infection. Conclusion: The high frequency of proteus mirabilis infection has been detected and the frequency was of females with complaint of urinary tract infection was high.

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Serum Immunoglobulin Response and Protection from Homologous Challenge by Proteus mirabilis in a Mouse Model of Ascending Urinary Tract Infection

Infection and Immunity ◽

10.1128/iai.67.12.6683-6687.1999 ◽

1999 ◽

Vol 67 (12) ◽

pp. 6683-6687 ◽

Cited By ~ 4

Author(s):

David E. Johnson ◽

Farah K. Bahrani ◽

C. Virginia Lockatell ◽

Cinthia B. Drachenberg ◽

J. Richard Hebel ◽

...

Keyword(s):

Urinary Tract Infection ◽

Urinary Tract ◽

Tract Infection ◽

Proteus Mirabilis ◽

Elevated Serum ◽

Serum Immunoglobulin ◽

Western Blots ◽

Serum Iga ◽

Isogenic Mutants ◽

Homologous Challenge

ABSTRACT We tested the hypothesis that experimental Proteus mirabilis urinary tract infection in mice would protect against homologous bladder rechallenge. Despite production of serum immunoglobulin G (IgG) and IgM (median titers of 1:320 and 1:80, respectively), vaccinated (infected and antibiotic-cured) mice did not show a decrease in mortality upon rechallenge; the survivors experienced only modest protection from infection (mean log10 number of CFU of P. mirabilisNalr HI4320 per milliliter or gram in vaccinated mice versus sham-vaccinated mice: urine, 100-fold less [3.5 versus 5.5;P = 0.13]; bladder, 100-fold less [3.1 versus 5.1;P = 0.066]; kidneys, 40-fold less [2.7 versus 4.3;P = 0.016]). Western blots using protein from the wild-type strain and isogenic mutants demonstrated antibody responses to MR/P and PMF fimbriae and flagella. There was no correlation between serum IgG or IgM levels and protection from mortality or infection. There was a trend toward elevated serum IgA titers and protection from subsequent challenge (P ≥ 0.09), although only a few mice developed significant serum IgA levels. We conclude that prior infection with P. mirabilis does not protect significantly against homologous challenge.

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Group DSalmonellaUrinary Tract Infection in an Immunocompetent Male

Case Reports in Infectious Diseases ◽

10.1155/2015/608632 ◽

2015 ◽

Vol 2015 ◽

pp. 1-2 ◽

Cited By ~ 4

Author(s):

Asad Jehangir ◽

Dilli Poudel ◽

Shoaib Bilal Fareedy ◽

Ahmed Salman ◽

Anam Qureshi ◽

...

Keyword(s):

Urinary Tract Infection ◽

Urinary Tract ◽

Benign Prostatic Hyperplasia ◽

Tract Infection ◽

Prostatic Hyperplasia ◽

Genitourinary Tract ◽

Watery Diarrhea ◽

Urinary Flow ◽

History Of ◽

Group D

A 62-year-old male with past medical history of benign prostatic hyperplasia presented to the emergency department with complaints of decreased urinary flow, inability to fully empty his bladder, and gross hematuria. Physical examination was unremarkable. Urinalysis revealed large amount of blood and more than 700 white blood cells suggesting a urinary tract infection. Urine culture grew group DSalmonellagreater than 100,000 colony-forming units per mL. He was prescribed 6 weeks of trimethoprim/sulfamethoxazole and had resolution of symptoms. Retrospectively, he reported a 3-day history of watery diarrhea about a week prior to onset of urinary symptoms that was presumed to be the hematogenous source in this case. Urinary tract infection from nontyphoidalSalmonella(NTS) is rare and is usually associated with immunosuppression, chronic diseases, such as diabetes or structural abnormalities of the genitourinary tract. Genitourinary tract abnormalities previously reported in the literature that predispose to nontyphoidalSalmonellaurinary tract infection include nephrolithiasis, chronic pyelonephritis, retrovesicular fistula, urethrorectal fistula, hydrocele, and post-TURP. We present an exceedingly uncommon case of 62-year-old male with group DSalmonellaurinary tract infection predisposed by his history of benign prostatic hyperplasia.

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