Deletions in the tetracycline resistance determinant reduce the thermosensitivity of A trfA(Ts) derivative of plasmid RP1 in Pseudomonas aeruginosa

1987 ◽  
Vol 138 (2) ◽  
pp. 151-164 ◽  
Author(s):  
M Rella ◽  
J.M Watson ◽  
C.M Thomas ◽  
D Haas
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Tetracycline Resistance ◽  
Resistance Determinant

scholarly journals Novel Tetracycline Resistance Determinant from the Oral Metagenome

2003 ◽  
Vol 47 (4) ◽  
pp. 1430-1432 ◽  
Author(s):  
M. L. Diaz-Torres ◽  
R. McNab ◽  
D. A. Spratt ◽  
A. Villedieu ◽  
N. Hunt ◽  
...  
Keyword(s):  
Resistance Gene ◽  
Tetracycline Resistance ◽  
Resistance Determinant ◽  
Tetracycline Resistance Gene ◽  
Major Drawback

ABSTRACT A major drawback of most studies on how bacteria become resistant to antibiotics is that they concentrate mainly on bacteria that can be cultivated in the laboratory. In the present study, we cloned part of the oral metagenome and isolated a novel tetracycline resistance gene, tet(37), which inactivates tetracycline.

scholarly journals Novel Tetracycline Resistance Determinant Isolated from an Environmental Strain of Serratia marcescens

2007 ◽  
Vol 73 (7) ◽  
pp. 2199-2206 ◽  
Author(s):  
Stuart A. Thompson ◽  
Elizabeth V. Maani ◽  
Angela H. Lindell ◽  
Catherine J. King ◽  
J. Vaun McArthur
Keyword(s):  
Serratia Marcescens ◽  
Efflux Pump ◽  
Tetracycline Resistance ◽  
Amino Acid Identity ◽  
Resistance Determinant ◽  
Mercury Resistance ◽  
Resistance Locus ◽  
Environmental Strain ◽  
Apparent Lack ◽  
E Coli

ABSTRACT Resistances to tetracycline and mercury were identified in an environmental strain of Serratia marcescens isolated from a stream highly contaminated with heavy metals. As a step toward addressing the mechanisms of coselection of heavy metal and antibiotic resistances, the tetracycline resistance determinant was cloned in Escherichia coli. Within the cloned 13-kb segment, the tetracycline resistance locus was localized by deletion analysis and transposon mutagenesis. DNA sequence analysis of an 8.0-kb region revealed a novel gene [tetA(41)] that was predicted to encode a tetracycline efflux pump. Phylogenetic analysis showed that the TetA(41) protein was most closely related to the Tet(39) efflux protein of Acinetobacter spp. yet had less than 80% amino acid identity with known tetracycline efflux pumps. Adjacent to the tetA(41) gene was a divergently transcribed gene [tetR(41)] predicted to encode a tetracycline-responsive repressor protein. The tetA(41)-tetR(41) intergenic region contained putative operators for TetR(41) binding. The tetA(41) and tetR(41) promoters were analyzed using lacZ fusions, which showed that the expression of both the tetA(41) and tetR(41) genes exhibited TetR(41)-dependent regulation by subinhibitory concentrations of tetracycline. The apparent lack of plasmids in this S. marcescens strain, as well as the presence of metabolic genes adjacent to the tetracycline resistance locus, suggested that the genes were located on the S. marcescens chromosome and may have been acquired by transduction. The cloned Tet 41 determinant did not confer mercury resistance to E. coli, confirming that Tet 41 is a tetracycline-specific efflux pump rather than a multidrug transporter.

The rarely reported tet(31) tetracycline resistance determinant is common in Gallibacterium anatis

2011 ◽  
Vol 149 (3-4) ◽  
pp. 497-499 ◽  
Author(s):  
Anders M. Bojesen ◽  
Ragnhild J. Bager ◽  
Dan Ifrah ◽  
Frank M. Aarestrup
Keyword(s):  
Tetracycline Resistance ◽  
Resistance Determinant

scholarly journals Activity of Tigecycline (GAR-936), a Novel Glycylcycline, against Enterococci in the Mouse Peritonitis Model

2003 ◽  
Vol 47 (2) ◽  
pp. 529-532 ◽  
Author(s):  
Esteban C. Nannini ◽  
Suresh R. Pai ◽  
Kavindra V. Singh ◽  
Barbara E. Murray
Keyword(s):  
Body Weight ◽  
Mouse Model ◽  
Protective Effect ◽  
Enterococcus Faecalis ◽  
Enterococcus Faecium ◽  
Tetracycline Resistance ◽  
Resistance Determinant ◽  
Subcutaneous Dose ◽  
Peritonitis Model

ABSTRACT A novel glycylcycline agent, tigecycline (GAR-936), was evaluated in vivo in the mouse model of peritonitis against three Enterococcus faecalis and four Enterococcus faecium isolates with different susceptibilities to vancomycin and tetracyclines, all of which were inhibited by ≤0.125 μg of tigecycline/ml. Using a single subcutaneous dose, tigecycline displayed a protective effect (50% protective dose, ≤5.7 mg/kg of body weight) against all strains tested, including two with Tn925 (from the Tn916 family), which contains the Tet(M) tetracycline resistance determinant, as well as VanA and VanB strains. As expected, tetracycline and minocycline were ineffective against the isolates carrying Tn925.

scholarly journals Construction and Use of Derivatives of Transposon Tn4001 That Function in Mycoplasma pulmonis andMycoplasma arthritidis

2000 ◽  
Vol 182 (15) ◽  
pp. 4343-4347 ◽  
Author(s):  
Kevin Dybvig ◽  
C. Todd French ◽  
LeRoy L. Voelker
Keyword(s):  
Tetracycline Resistance ◽  
Chloramphenicol Acetyltransferase ◽  
Resistance Determinant ◽  
Mycoplasma Pulmonis ◽  
Functional Failure ◽  
General Utility ◽  
Lacz Activity ◽  
Gentamicin Resistance ◽  
Chloramphenicol Acetyltransferase Gene ◽  
Derivatives Of

ABSTRACT Previous attempts to introduce transposon Tn4001 intoMycoplasma pulmonis and Mycoplasma arthritidishave not been successful, possibly due to functional failure of the transposon's gentamicin resistance determinant. Tn4001Cand Tn4001T were constructed, respectively, by insertion of a chloramphenicol acetyltransferase gene and the tetMtetracycline resistance determinant into Tn4001. Both Tn4001C and Tn4001T transposed in M. pulmonis, and Tn4001T transposed in M. arthritidis. The incorporation of a Tn4001Tderivative that contained lacZ into eitherMycoplasma species resulted in transformants with readily detectable LacZ activity. Tn4001T may be of general utility for use as a mycoplasma cloning vehicle because tetMfunctions in all species of Mycoplasma examined thus far.

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