Association of β-1,3-glucanase activity and isoform pattern with systemic resistance to blue mould in tobacco induced by stem injection with Peronospora tabacina or leaf inoculation with tobacco mosaic virus

1991 ◽  
Vol 39 (1) ◽  
pp. 25-39 ◽  
Author(s):  
S.Q. Pan ◽  
X.S. Ye ◽  
J. Kuć
Keyword(s):  
Tobacco Mosaic Virus ◽  
Mosaic Virus ◽  
Systemic Resistance ◽  
Peronospora Tabacina ◽  
Glucanase Activity ◽  
Blue Mould ◽  
Stem Injection

Acquired systemic resistance to tobacco mosaic virus in Nicotiana tabacum evoked by stem injection with Peronospora tabacina Adam

1963 ◽  
Vol 14 (3) ◽  
pp. 315 ◽  
Author(s):  
M Mandryk
Keyword(s):  
Nicotiana Tabacum ◽  
Tobacco Mosaic Virus ◽  
Mosaic Virus ◽  
Systemic Resistance ◽  
Peronospora Tabacina ◽  
Nicotiana Tabacum L ◽  
Stem Injection ◽  
The Difference ◽  
And Control

Resistance to tobacco mosaic virus (TMV) was induced in leaves of Nicotiana tabacum L. (cv. Xanthi and cv. Havana 423) by injecting spore suspensions of Peronospora tabacina Adam into the stems of plants. TMV lesions that developed on the leaves of stem-injected plants were 62% smaller and 32% fewer in number than their counterparts in control plants. While the difference in numbers of lesions on the leaves between injected and control plants decreased from the bottom to the top of the plant, the difference in the size of lesions was maintained at all leaf levels.

Biochemical changes in cell walls and cellular responses of tobacco leaves related to systemic resistance to blue mold (Peronospora tabacina) induced by tobacco mosaic virus

1992 ◽  
Vol 70 (1) ◽  
pp. 49-57 ◽  
Author(s):  
X. S. Ye ◽  
U. Järlfors ◽  
S. Tuzun ◽  
S. Q. Pan ◽  
J. Kuc
Keyword(s):  
Cell Wall ◽  
Tobacco Mosaic Virus ◽  
Mosaic Virus ◽  
Cell Walls ◽  
Induced Systemic Resistance ◽  
Host Cells ◽  
Systemic Resistance ◽  
Blue Mold ◽  
Peronospora Tabacina ◽  
Fungal Hyphae

Inoculation of lower leaves of tobacco cultivar Ky 14, which carries the N gene for resistance to tobacco mosaic virus, with tobacco mosaic virus induced systemic resistance to Peronospora tabacina and a systemic accumulation of cell wall hydroxyproline. Hydroxyproline increased significantly 12 days after induction with tobacco mosaic virus, and more so after challenge with P. tabacina. During this period, hydroxyproline levels in the control plants remained unchanged. Four salt-soluble cell wall proteins were systemically induced. These proteins were not β-1,3-glucanases, chitinases, or hydroxyproline-rich glycoproteins. Light microscopy showed that blue mold development in the induced plants was severely restricted 2 days after challenge; some fungal hyphae were disorganized near the center of infection sites, and adjacent host cells were plasmolyzed and a few collapsed 3 days after challenge. All infection sites in the induced plants were associated with necrotic cells 5–6 days after challenge. Electron microscopy revealed that damage to fungal hyphae, plasmolysis and shrinking of infected cells, and more electron-opaque host cell walls and wall appositions were characteristics of induced resistance. Key words: induced systemic resistance, Nicotiana tabacum, blue mold (Peronospora tabacina).

Induction of systemic resistance against tobacco mosaic virus by Ningnanmycin in tobacco

2014 ◽  
Vol 111 ◽  
pp. 14-18 ◽  
Author(s):  
Yongguang Han ◽  
Yue Luo ◽  
Shirong Qin ◽  
Lei Xi ◽  
Bo Wan ◽  
...  
Keyword(s):  
Tobacco Mosaic Virus ◽  
Mosaic Virus ◽  
Systemic Resistance

scholarly journals Exogenous Application of Harpin Protein Hpa1 onto Pinellia ternata Induces Systemic Resistance Against Tobacco Mosaic Virus

2020 ◽  
Vol 110 (6) ◽  
pp. 1189-1198
Author(s):  
Defu Wang ◽  
Baoxia Wang ◽  
Jiangran Wang ◽  
Shuting Wang ◽  
Weiyu Wang ◽  
...  
Keyword(s):  
Disease Resistance ◽  
Tobacco Mosaic Virus ◽  
Mosaic Virus ◽  
Large Scale ◽  
Pathogen Resistance ◽  
Field Application ◽  
Systemic Resistance ◽  
Pinellia Ternata ◽  
Related Substances ◽  
Harpin Protein

The harpin protein Hpa1 has various beneficial effects in plants, such as promoting plant growth and inducing pathogen resistance. Our previous study found that Hpa1 could significantly alleviate the mosaic symptoms of tobacco mosaic virus (TMV) in Pinellia ternata, indicating that Hpa1 can effectively stimulate resistance. Here, the potential mechanism of disease resistance and field applicability of Hpa1 against TMV in P. ternata were further investigated. The results showed that 15 µg ml−1 Hpa1 had stronger antiviral activity than the control, and its protective effect was better than its curative effect. Furthermore, Hpa1 could significantly induce an increase in defense-related enzyme activity, including polyphenol oxidase, peroxidase, catalase, and superoxide dismutase, as well as increase the expression of disease resistance-related genes (PR1, PR3, PR5, and PDF1.2). Concurrently, Hpa1 significantly increased the content of some disease resistance-related substances, including hydrogen peroxide, phenolics, and callose, whereas the content of malondialdehyde was reduced. In addition, field application analysis demonstrated that Hpa1 could effectively elicit a defense response against TMV in P. ternata. Our findings propose a mechanism by which Hpa1 can prevent TMV infection in Pinellia by inducing systemic resistance, thereby providing an environmentally friendly approach for the use of Hpa1 in large-scale applications to improve TMV resistance in Pinellia.

scholarly journals Salicylic Acid and Jasmonic Acid Are Essential for Systemic Resistance Against Tobacco mosaic virus in Nicotiana benthamiana

2014 ◽  
Vol 27 (6) ◽  
pp. 567-577 ◽  
Author(s):  
Feng Zhu ◽  
De-Hui Xi ◽  
Shu Yuan ◽  
Fei Xu ◽  
Da-Wei Zhang ◽  
...  
Keyword(s):  
Salicylic Acid ◽  
Jasmonic Acid ◽  
Tobacco Mosaic Virus ◽  
Mosaic Virus ◽  
Nicotiana Benthamiana ◽  
Foliar Application ◽  
Mass Spectrometry Analysis ◽  
Systemic Resistance ◽  
Long Distance ◽  
Resistance Response

Systemic resistance is induced by pathogens and confers protection against a broad range of pathogens. Recent studies have indicated that salicylic acid (SA) derivative methyl salicylate (MeSA) serves as a long-distance phloem-mobile systemic resistance signal in tobacco, Arabidopsis, and potato. However, other experiments indicate that jasmonic acid (JA) is a critical mobile signal. Here, we present evidence suggesting both MeSA and methyl jasmonate (MeJA) are essential for systemic resistance against Tobacco mosaic virus (TMV), possibly acting as the initiating signals for systemic resistance. Foliar application of JA followed by SA triggered the strongest systemic resistance against TMV. Furthermore, we use a virus-induced gene-silencing–based genetics approach to investigate the function of JA and SA biosynthesis or signaling genes in systemic response against TMV infection. Silencing of SA or JA biosynthetic and signaling genes in Nicotiana benthamiana plants increased susceptibility to TMV. Genetic experiments also proved the irreplaceable roles of MeSA and MeJA in systemic resistance response. Systemic resistance was compromised when SA methyl transferase or JA carboxyl methyltransferase, which are required for MeSA and MeJA formation, respectively, were silenced. Moreover, high-performance liquid chromatography–mass spectrometry analysis indicated that JA and MeJA accumulated in phloem exudates of leaves at early stages and SA and MeSA accumulated at later stages, after TMV infection. Our data also indicated that JA and MeJA could regulate MeSA and SA production. Taken together, our results demonstrate that (Me)JA and (Me)SA are required for systemic resistance response against TMV.

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