The repair of ultraviolet light-induced DNA damage in the halophilic archaebacteria, Halobacterium cutirubrum, Halobacterium halobium and Haloferax volcanii

1996 ◽  
Vol 364 (1) ◽  
pp. 25-32 ◽  
Author(s):  
Shirley McCready
1990 ◽  
Vol 16 (1) ◽  
pp. 37-43 ◽  
Author(s):  
Christine A. Huselton ◽  
Helene Z. Hill ◽  
J. P. O'Neill
Keyword(s):  

1989 ◽  
Vol 35 (1) ◽  
pp. 148-152 ◽  
Author(s):  
Steven W. Cline ◽  
Wan L. Lam ◽  
Robert L. Charlebois ◽  
Leonard C. Schalkwyk ◽  
W. Ford Doolittle

We present a practical description of polyethylene glycol mediated spheroplast transformation of Halobacterium halobium and Halobacterium volcanii. This method has been applied to phage DNA transfection, plasmid DNA transformation, and transformation with linear fragments of high molecular weight genomic DNA. Efficient spheroplast regeneration allows uncomplicated recovery of transformed progeny. Transformations can be performed equally well using fresh or frozen cell preparations. These methods should find application in molecular cloning, genetic fine mapping, and strain construction.Key words: archaebacteria, Halobacterium, transformation methods, spheroplast.


Author(s):  
K.G.K. Cieminis ◽  
V.M. Ranc̆elienė ◽  
A.J. Prijalgauskienė ◽  
N.V. Tiunaitienė ◽  
A.M. Rudzianskaitė ◽  
...  

Sign in / Sign up

Export Citation Format

Share Document