Nematicidal Activity of the Endophyte Serratia ureilytica against Nacobbus aberrans in Chili Plants (Capsicum annuum L.) and Identification of Genes Related to Biological Control

The genus Serratia is widely distributed in soil, water, plants, animals, invertebrates, and humans. Some species of this genus have antifungal, antibacterial, and nematicidal activity. In this work, the nematicidal activity of the endophytic strain of Serratia sp. in chili, Capsicum annuum L., is reported, where at a bacterial concentration of 4 × 109 cel/mL, the penetration of nematodes into the roots significantly decreased by 91 and 55% at 7 and 21 days after inoculation. This bacterial concentration also significantly decreased the number of galls, eggs, egg masses and reproduction factor produced by Nacobbus aberrans in Chili plants, with respect to the control where this bacterial strain was not applied. In the analysis of the genome of the strain, based on average nucleotide identity (ANI), the isolate could be affiliated to the species Serratia ureilytica. The size of the genome is 5.4 Mb, with a 59.3% content of GC. Genes related to the synthesis of chitinases, siderophores, proteases C, serralisins, hemolysin, and serrawettin W2 that have been reported for biocontrol of nematodes were identified in the genome. It is the first report of Serratia ureilytica with nematicidal activity. Based on these results of nematicidal activity, this strain can be evaluated in the field as an alternative in the biocontrol of Nacobbus aberrans in chili cultivation.

Rhizobacteria Impact Colonization of Listeria monocytogenes on Arabidopsis thaliana Roots

Listeria monocytogenes is ubiquitous in the environment, being found in or on soil, water, plants, and wildlife. However, little is known about the requirements for L. monocytogenes ’ existence in these settings.

The effectiveness of filtration and phytoremediation with combination of aquatic plants in wastewater treatment of Sasirangan industry

Most of Sasirangan fabric industry in South Kalimantan have not carried out an optimal wastewater treatment effort. In order to improve the quality of the waste, a combination of filtration and phytoremediation using the symbiosis of water hyacinth and Jeringau plants were applied. This study aimed to analyze the effectiveness of the filtration and phytoremediation processes using a combination of the two aquatic plants. The method used in this study was an experimental method with the treatment of a comparison of water hyacinth and Jeringau, namely 1: 3, 1: 1, and 3: 1 (w/w). The research stages are the acclimatization of the two aquatic plants for ten days, filtration with sand, gravel, and coconut fibers, and phytoremediation. The parameters tested in this study were BOD, COD, and TSS. The results showed that the combination of filtration and phytoremediation with the combination of the two water plants was able to improve the quality of sasirangan’s effluent. The best treatment for BOD and TSS was the combination of water hyacinth and Jeringau 1:1 (w/w) with the effectiveness obtained was 75.57% for BOD; and 63.93% for TSS, and the combination of water hyacinth and Jeringau at 3:1 (w/w) for COD with the effectiveness of 87.20% for COD.

Game Analysis on Profits of Water Diversion Project Supply Chain Under the Overall Loss of Supply Chain in Undeveloped Areas

This paper compares and analyzes the effects of different amounts of government subsidies and subsidy patterns on water supply, pricing, and profit distribution, and takes water diversion project supply chain composed of water transfer company and the water work as the study object, using financial accounting method innovatively, establishing non-cooperative and cooperative game models of two under government subsidies. The results show that as the number of subsidies increased, the optimal amount of water supply and the profits increased, but the price of water work decreased. When the amount of subsidy stays same, the price of water plant also stays same. However, the price of the water transfer company is constantly changing, the higher the proportion of subsidies it received, the lower the price. In terms of data, the authors take a practical example – the Tao River Water Diversion Project to analyze. This paper’s limitation is that the conclusion is based on a single water transfer company and a single water plant as the research object. However, the actual situation of the water transfer project is that a water transfer company faces a complex supply chain network formed by many water plants and farmers’ water users’ associations.

PURIFICATION OF AIR BY LAKSHAHARIDRAADI YOGA AND SOME MEASURES TO REVERSE THE IMPACT OF POLLUTED AIR

Environment plays a very significant role in human civilization. Human beings have close relations with the bio- sphere in which they live. The whole environment and ecology consisting of earth, air, water, plants and animals provide the necessary and sufficient conditions for sustaining human life. In Yajurveda and Vedic sages pure wa- ter, air etc. are the roots to good health and happiness and hence they considered all these as gods. The Vedic peo- ple desired to live a life of hundred years and this wish can be fulfilled only when the environment will be unpol- luted, clean, and peaceful. Today, the environmental crisis is a tremendous problem for the whole world as it is suffering from environmental pollution. Therefore, today environmental problems have been the object of discus- sion everywhere from village to parliament. Air Pollution is by far the most rapidly growing problem of modern technological society. Although the world has already addressed this issue and has also taken steps to minimize it a lot more than this is needed. The damage which our hunger for ease of living had already caused is irreparable by just making laws, rules & regulations. We need strong implementation of it as well as general awareness and contribution from everyone. Ayurveda can play a vital role in fighting this battle for cleaning the air and minimiz- ing air pollution; it could even emerge as ‘SanjeevaniBooti’. There's no need of proving the worth of Vedic science to our society as it is now worldwide accepted and part of everyone’s life. Recent Ayurveda studies have shown positive results in dealing effectively with the evil of air pollution.

Riparian Tree Vegetation Diversity Salah Nama Island in Banyuasin, South Sumatera

Highlight Research:Riparian plants play an important role in maintaining the balance of an aquatic ecosystem.The diversity of riparian aquatic plants on the island of Salah Nama BanyuasinData collection on riparian plants used the quadratic transect methodThe results showed that there were 21 types of riparian aquatic plants in 16 families.The highest composition is in the Lythraceae family, namely Sonneratia acidaAbstractRiparian plants play an important role in maintaining the balance of an aquatic ecosystem. The missing plant components from a water can cause sedimentation and change the microhabitat in these waters. The purpose of the study is to determine the diversity of riparian aquatic plants on the island of Salah Nama Banyuasin. The research was conducted in August 2016, November 2016, and January 2017 at Salah Nama Island Mariana Ilir Banyuasin 1, South Sumatra. Sampling was done by using the purposive sampling method. Determination of the sampling location used GPS (Global positioning system). Observation stations consisted of 5 sampling locations. The method of taking water plants was done in exploratory way. Data collection on riparian plants used the quadratic transect method measuring 1 m x 1 m. Samples of riparian plants were taken then wrapped with newspaper or paper and put into large plastic, labeled then taken to the testing laboratory Institute Inland fisheries and extension, Palembang. The results showed that there were 21 types of riparian aquatic plants in 16 families. The highest composition is in the Lythraceae family, namely Sonneratia acida. The diversity index of aquatic plants ranges from 1 <H'<3 in the stable condition category. Based on the results of the study, the riparian plant species that dominated the most were Sonneratia sp. The conclusion that can be drawn is that the diversity of riparian plants on the island of Salah Nama is in the medium category, the dominant riparian plants are Sonneratia sp.

Improved YOLO Based Detection Algorithm for Floating Debris in Waterway

Various floating debris in the waterway can be used as one kind of visual index to measure the water quality. The traditional image processing method is difficult to meet the requirements of real-time monitoring of floating debris in the waterway due to the complexity of the environment, such as reflection of sunlight, obstacles of water plants, a large difference between the near and far target scale, and so on. To address these issues, an improved YOLOv5s (FMA-YOLOv5s) algorithm by adding a feature map attention (FMA) layer at the end of the backbone is proposed. The mosaic data augmentation is applied to enhance the detection effect of small targets in training. A data expansion method is introduced to expand the training dataset from 1920 to 4800, which fuses the labeled target objects extracted from the original training dataset and the background images of the clean river surface in the actual scene. The comparisons of accuracy and rapidity of six models of this algorithm are completed. The experiment proves that it meets the standards of real-time object detection.

When Nature Gets Thirsty

Water is the fountain of life. Without water, nothing grows, including us! But not everything in nature can have all the water it needs, all the time. If plants and animals get too thirsty, they get stressed. When nature dries out, dangerous conditions like droughts and wildfires can happen. It is important for national park managers to understand where and when nature is thirsty. As scientists, we track how much water falls as rain and snow. Then we subtract how much water leaves through rivers, groundwater, and evaporation. The leftover water can be used by plants and animals. We compare how much water plants want to how much water is available, so we can know how thirsty plants are. The hotter it is, the faster water disappears. By knowing when and where nature is thirsty, park managers can take actions to help protect parks.

Bacillus cereus in Packaging Material: Molecular and Phenotypical Diversity Revealed

The Bacillus cereus group has been isolated from soils, water, plants and numerous food products. These species can produce a variety of toxins including several enterotoxins [non-hemolytic enterotoxin (Nhe), hemolysin BL (Hbl), cytotoxin K, and enterotoxin FM], the emetic toxin cereulide and insecticidal Bt toxins. This is the first study evaluating the presence of B. cereus in packaging material. Among 75 different isolates, four phylogenetic groups were detected (II, III, IV, and VI), of which the groups III and IV were the most abundant with 46.7 and 41.3%, respectively. One isolate was affiliated to psychrotolerant group VI. Growth experiments showed a mesophilic predominance. Based on PCR analysis, nhe genes were detectable in 100% of the isolates, while hbl genes were only found in 50.7%. The cereulide encoding gene was found in four out of 75 isolates, no isolate carried a crystal toxin gene. In total, thirteen different toxin gene profiles were identified. We showed that a variety of B. cereus group strains can be found in packaging material. Here, this variety lies in the presence of four phylogenetic groups, thirteen toxin gene profiles, and different growth temperatures. The results suggest that packaging material does not contain significant amounts of highly virulent strains, and the low number of cereulide producing strains is in accordance with other results.