μ-Opioid receptor-regulated adenylate cyclase activity in primary cultures of rat striatal neurons upon chronic morphine exposure

1991 ◽  
Vol 208 (2) ◽  
pp. 105-111 ◽  
Author(s):  
Bernard J. Van Vliet ◽  
Taco J. De Vries ◽  
George Wardeh ◽  
Arie H. Mulder ◽  
Anton N.M. Schoffelmeer
Keyword(s):  
Adenylate Cyclase ◽  
Opioid Receptor ◽  
Primary Cultures ◽  
Cyclase Activity ◽  
Adenylate Cyclase Activity ◽  
Striatal Neurons ◽  
Chronic Morphine ◽  
Μ Opioid Receptor

Adenosine and its analogue ( − )-N6-R-phenyl-isopropyladenosine modulate anterior pituitary adenylate cyclase activity and prolactin secretion in the rat

1990 ◽  
Vol 5 (1) ◽  
pp. 69-76 ◽  
Author(s):  
G. Schettini ◽  
E. Landolfi ◽  
O. Meucci ◽  
T. Florio ◽  
M. Grimaldi ◽  
...  
Keyword(s):  
Adenylate Cyclase ◽  
Anterior Pituitary ◽  
Primary Cultures ◽  
Prolactin Secretion ◽  
Cyclase Activity ◽  
Adenylate Cyclase Activity ◽  
Pituitary Cells ◽  
Prolactin Release ◽  
Low Concentrations ◽  
High Concentrations

ABSTRACT The effect of adenosine and its analogue ( − )-N6-R-phenylisopropyladenosine (PIA) on both anterior pituitary adenylate cyclase activity and prolactin secretion was examined in the rat. Adenosine inhibited basal adenylate cyclase activity in a dose-dependent manner and also reduced the stimulation of the enzyme by vasoactive intestinal peptide (VIP). Likewise, in primary cultures of anterior pituitary cells, adenosine decreased prolactin secretion in both basal and VIP-stimulated conditions. In perifusion experiments, adenosine also inhibited prolactin release in both basal and TRH-stimulated conditions. PIA produced a biphasic pattern of response of basal adenylate cyclase activity, being inhibitory at low and stimulatory at high concentrations. In VIP-stimulated conditions, low concentrations of PIA inhibited both adenylate cyclase activity and prolactin release from primary cultures of pituitary cells, while no additive stimulatory effect was seen at high concentrations. Similarly, low concentrations of PIA reduced both basal and TRH-stimulated prolactin release from perifused pituitaries, while increasing PIA concentrations restored prolactin release. These data show that adenosine affects basal and stimulated prolactin secretion from anterior pituitary cells. Adenosine receptors seem to be coupled to the adenylate cyclase system in the anterior pituitary gland, suggesting a possible relationship between the effect of adenosine on adenylate cyclase activity and prolactin secretion.

scholarly journals μ-, δ- and κ-opioid receptor-mediated inhibition of neurotransmitter release and adenylate cyclase activity in rat brain slices: studies with fentanyl isothiocyanate

1988 ◽  
Vol 154 (2) ◽  
pp. 169-178 ◽  
Author(s):  
Anton N.M. Schoffelmeer ◽  
Kenner C. Rice ◽  
Arthur E. Jacobson ◽  
Johannus G. Van Gelderen ◽  
François Hogenboom ◽  
...  
Keyword(s):  
Adenylate Cyclase ◽  
Rat Brain ◽  
Opioid Receptor ◽  
Neurotransmitter Release ◽  
Brain Slices ◽  
Cyclase Activity ◽  
Adenylate Cyclase Activity

Differential effects of chronic agonist administration on μ-opioid receptor- and muscarinic receptor-regulated adenylate cyclase in rat striatal neurons

Life Sciences ◽  
1992 ◽  
Vol 51 (10) ◽  
pp. PL89-PL94 ◽  
Author(s):  
Bernard J. Van Vliet ◽  
Corinne H. Dotman ◽  
George Wardeh ◽  
Arie H. Mulder ◽  
Anton N.M. Schoffelmeer
Keyword(s):  
Adenylate Cyclase ◽  
Muscarinic Receptor ◽  
Opioid Receptor ◽  
Striatal Neurons ◽  
Differential Effects ◽  
Μ Opioid Receptor

Cytochemical Evidence for Presynatic β-Adrenergic Regulation of Secretion in the Developing Rat Submandibular Gland

1977 ◽  
Vol 35 ◽  
pp. 430-431
Author(s):  
L.S. Cutler
Keyword(s):  
Cyclic Amp ◽  
Adenylate Cyclase ◽  
Submandibular Gland ◽  
Neonatal Rat ◽  
Cyclase Activity ◽  
Adenylate Cyclase Activity ◽  
Parotid Glands ◽  
Adrenergic Agonist ◽  
Rat Submandibular Gland

Many studies previously have shown that the B-adrenergic agonist isoproterenol and the a-adrenergic agonist norepinephrine will stimulate secretion by the adult rat submandibular (SMG) and parotid glands. Recent data from several laboratories indicates that adrenergic agonists bind to specific receptors on the secretory cell surface and stimulate membrane associated adenylate cyclase activity which generates cyclic AMP. The production of cyclic AMP apparently initiates a cascade of events which culminates in exocytosis. During recent studies in our laboratory it was observed that the adenylate cyclase activity in plasma membrane fractions derived from the prenatal and early neonatal rat submandibular gland was retractile to stimulation by isoproterenol but was stimulated by norepinephrine. In addition, in vitro secretion studies indicated that these prenatal and neonatal glands would not secrete peroxidase in response to isoproterenol but would secrete in response to norepinephrine. In contrast to these in vitro observations, it has been shown that the injection of isoproterenol into the living newborn rat results in secretion of peroxidase by the SMG (1).

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