High-density lipoprotein inhibits the production of platelet-activating factor acetylhydrolase by HEPG2 cells

1994 ◽  
Vol 1 ◽  
pp. 431
Author(s):  
K. Satoh ◽  
H. Yoshida ◽  
M. Koyama ◽  
M. Hiramoto ◽  
S. Takamatsu
Keyword(s):  
High Density Lipoprotein ◽  
Hepg2 Cells ◽  
Platelet Activating Factor ◽  
Density Lipoprotein ◽  
High Density ◽  
Platelet Activating Factor Acetylhydrolase

Apolipoproteins C-II and C-III inhibit selective uptake of low- and high-density lipoprotein cholesteryl esters in HepG2 cells

2005 ◽  
Vol 37 (6) ◽  
pp. 1308-1318 ◽  
Author(s):  
Karine Huard ◽  
Philippe Bourgeois ◽  
David Rhainds ◽  
Louise Falstrault ◽  
Jeffrey S. Cohn ◽  
...  
Keyword(s):  
High Density Lipoprotein ◽  
Hepg2 Cells ◽  
Density Lipoprotein ◽  
High Density ◽  
Cholesteryl Esters ◽  
Selective Uptake

Hydrolysis of Platelet-Activating Factor by Purified High-Density Lipoprotein Associated Paraoxonase

1995 ◽  
Vol 89 (s33) ◽  
pp. 16P-16P
Author(s):  
M.I. MacKness ◽  
S. Arrol ◽  
C.A. Abbott ◽  
P.N. Durrington
Keyword(s):  
High Density Lipoprotein ◽  
Platelet Activating Factor ◽  
Density Lipoprotein ◽  
High Density ◽  
Hydrolysis Of

scholarly journals Identification of Novel Human High-Density Lipoprotein Receptor Up-regulators Using a Cell-Based High-Throughput Screening Assay

2007 ◽  
Vol 12 (2) ◽  
pp. 211-219 ◽  
Author(s):  
Yuan Yang ◽  
Zhongbing Zhang ◽  
Wei Jiang ◽  
Lei Gao ◽  
Guiyu Zhao ◽  
...  
Keyword(s):  
High Density Lipoprotein ◽  
Cell Line ◽  
High Throughput ◽  
Hepg2 Cells ◽  
High Throughput Screening ◽  
Density Lipoprotein ◽  
High Density ◽  
Luciferase Reporter ◽  
Stable Cell Line ◽  
Active Compounds

Scavenger receptor class B type I (SR-BI) is the high-affinity high-density lipoprotein (HDL) receptor, and CLA-1 is the human homologue of the murine SR-BI. CLA-1/SR-BI receptor has been suggested as a new preventative and/or therapeutic target for atherosclerosis due to its pivotal role in overall HDL cholesterol (HDL-C) metabolism and its antiatherogenic activity in vivo. To search for active compounds that can increase CLA-1 transcription, a novel cell-based assay was developed for application in high-throughput screening (HTS). Human hepatoma HepG2 cells were transfected with a CLA-1-promoter-luciferase reporter gene construct, and the stable transfected cell line was selected and named CLAp-LUC HepG2. With rosiglitazone as a positive control, this stable cell line was used to establish a specific CLA-1 gene expression assay in a 96-well microplate format. The evaluating parameter Z' value of 0.64 showed that this cell-based HTS assay was robust and reliable. Screening of 6000 microbial secondary metabolite crude extracts identified 8 positive strains. Between 2 identified CLA-1 up-regulators produced by actinomycete strain 04-4776, 4776B may stimulate not only the expression of CLA-1 on the transcriptional and translational levels but also the activity of CLA-1 to uptake the HDL-C in HepG2 cells. The active compounds originated from this HTS assay may be developed to drug candidates or lead compounds for new antiatherosclerosis agents.

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