IMPROVEMENTS IN THE DETERMINATION METHOD OF METHYL MERCURY IN FISH TISSUES & THE RATIO OF METHYL MERCURY TO TOTAL MERCURY IN FISH TISSUES

1973 ◽  
pp. 819-829
Author(s):  
SYOZO FUKUI ◽  
KATSUHIKO TANAKA ◽  
SABURO KANNO ◽  
TYUNOSIN UKITA
Keyword(s):  
Methyl Mercury ◽  
Total Mercury ◽  
Determination Method ◽  
Fish Tissues ◽  
Mercury In Fish

Rapid Semimicro Method for the Determination of Methyl Mercury in Fish Tissue

1972 ◽  
Vol 55 (3) ◽  
pp. 583-589 ◽  
Author(s):  
J F Uthe ◽  
J Solomon ◽  
B Grift
Keyword(s):  
Gas Chromatography ◽  
Thin Layer ◽  
Methyl Mercury ◽  
Total Mercury ◽  
Aqueous Ethanol ◽  
Fish Tissue ◽  
Mercuric Iodide ◽  
Mercury In Fish ◽  
Layer Chromatography

Abstract A fast semimicro method for the determination of methyl mercury in fish tissue is described. The procedure involves extracting the methyl mercury into toluene as methyl mercuric bromide, partitioning the bromide into aqueous ethanol as a thiosulfate complex, and re-extracting into benzene as methyl mercuric iodide. Methyl mercury is quantitated with gas chromatography. The method is sensitive to 0.01 ppm. Recoveries of added methyl mercury were 99% and the presence of methyl mercury in the final extract was shown by thin layer chromatography and gas chromatography of the thin layer spot. A variety of mercurial compounds do not interfere in the analyses. The amounts of both methyl and total mercury found in a variety of tissues of aquatic animals are compared. The presence of a demethylase in seal is suggested by the findings of high levels of nonmethyl mercury. Additional cleanup by column chromatography on Florisil was necessary with certain samples. The gas chromatographic columns were kept operational by the intermittent injection of 3M potassium iodide. Due to column bleed and resulting detector contamination, the use of the easily cleaned concentric tube electron capture detector is recommended.

scholarly journals Do the total mercury concentrations detected in fish from Czech ponds represent a risk for consumers?

2022 ◽  
Vol 12 (1) ◽  
Author(s):  
Sehonova Pavla ◽  
Harustiakova Danka ◽  
Mikula Premysl ◽  
Medkova Denisa ◽  
Malacova Kristyna ◽  
...  
Keyword(s):  
Aquatic Ecosystems ◽  
Total Mercury ◽  
Absorption Spectrometry ◽  
Mercury Content ◽  
Predatory Fish ◽  
Average Content ◽  
Fish Tissues ◽  
The Czech Republic ◽  
Mercury In Fish ◽  
Commission Regulation

AbstractMercury is one of the important pollutants of the environment. Therefore, it’s necessary to monitor quantity of mercury especially in aquatic ecosystems. The main goal of the presented study was to compare the content of total mercury in tissues of fish coming from the Czech Republic, an important carp exporter, with focus on comparison of mercury content between 3 different ponds, its comparison between different fish species and between different tissues of the same species, and estimation whether the mercury content in tissues meets the limit given in the Commission Regulation (EC) No. 1881/2006 or not. Total mercury concentration was measured in 90 fish specimen sampled from three ponds (Velky Kocelovicky, Mysliv and Zehunsky) in autumn 2018. The values of total mercury in fish tissues was measured by atomic absorption spectrometry. The content of total mercury in the tissues decreased as follows: muscle > liver > gonads > scales. The highest average content of total mercury in muscle was 0.1517 ± 0.0176 mg/kg coming from pike caught in Velky Kocelovicky pond. In contrast, the lowest average content of total mercury in muscle 0.0036 ± 0.0003 mg/kg was found in carp tissue coming from the locality of Zehunsky pond. We confirmed that the predatory fish are more exposed to mercury than non-predatory fish. None of the monitored localities exceeded the set regulatory limit. Thus, our study shows that fish coming from these ponds are safe in terms of total mercury content.

Importance of Wetlands as Sources of Methyl Mercury to Boreal Forest Ecosystems

1994 ◽  
Vol 51 (5) ◽  
pp. 1065-1076 ◽  
Author(s):  
Vincent L. St. Louis ◽  
John W. M. Rudd ◽  
Carol A. Kelly ◽  
Ken G. Beaty ◽  
Nicholas S. Bloom ◽  
...  
Keyword(s):  
Boreal Forest ◽  
Methyl Mercury ◽  
Total Mercury ◽  
Forest Ecosystems ◽  
Runoff Water ◽  
Riparian Wetland ◽  
Mercury In Fish ◽  
High Concentrations ◽  
Mercury Production ◽  
Upland Catchments

Wetlands were found to be important sources of methyl mercury to the boreal forest ecosystem. Yields of methyl mercury were about 26–79 times higher from wetland portions of catchments (1.84–5.55 mg∙ha−1∙yr−1) than from purely upland areas (0.07 mg∙ha−1∙yr−1). Mass-balance estimates using methyl mercury inputs in wet deposition and outputs in runoff water indicated that purely upland catchments and lakes were sites of methyl mercury retention or demethylation, while catchments with wetland areas were sites of net methyl mercury production. These observations may explain the high concentrations of mercury in fish taken from lakes that are high in colour because they receive water from wetlands. There was no relationship between the concentration of total mercury and the concentration of methyl mercury in runoff water. Total mercury yields were low from a wetland-dominated catchment, higher from a combination upland/riparian wetland catchment, and highest from a purely upland catchment. The opposite was true for methyl mercury yields from these same catchments. This indicates that environmental factors other than total mercury concentration are controlling the production and loss of methyl mercury from catchments.

Gas Chromatographic Determination of Methyl Mercury in Fish, Sediment, and Water

1973 ◽  
Vol 56 (6) ◽  
pp. 1297-1303 ◽  
Author(s):  
James E Longbottom ◽  
Ronald C Dressman ◽  
James J Lichtenberg
Keyword(s):  
Water Samples ◽  
Methyl Mercury ◽  
Total Mercury ◽  
Inorganic Mercury ◽  
Chromatographic Determination ◽  
Chloride Salt ◽  
Mercury In Fish ◽  
Cleanup Procedure ◽  
Very High

Abstract Methyl mercury is extracted as the bromide salt from fish and sediment and as the chloride salt from water samples. All extracts are treated with a common cleanup procedure that results in the conversion of methyl mercury to the iodide salt for electron capture gas chromatographic analysis. Recoveries ranged from an average of 88.5% for water samples to averages of 95.5 and 96.3% for perch and sediments. Methods for controlling contaminants and interferences are discussed for all phases of the method. Particular problems encountered were column poisoning and detector poisoning. When the method was applied to sediment samples collected from a polluted river, a correlation could be established between total mercury and methyl mercury when the concentration of total mercury was in the 0–10 μg/g region. For samples of very high inorganic mercury, the correlation failed.

scholarly journals Mercury distribution in different tissues and trophic levels of fish from a tropical reservoir, Brazil

2009 ◽  
Vol 7 (4) ◽  
pp. 751-758 ◽  
Author(s):  
Daniele Kasper ◽  
Elisabete Fernandes Albuquerque Palermo ◽  
Ana Carolina Monteiro Iozzi Dias ◽  
Gustavo Luiz Ferreira ◽  
Rafael Pereira Leitão ◽  
...  
Keyword(s):  
Trophic Level ◽  
Total Mercury ◽  
Inorganic Mercury ◽  
Trophic Levels ◽  
Southeastern Brazil ◽  
Organic Form ◽  
Fish Tissues ◽  
Tropical Reservoir ◽  
Level Increase ◽  
Carnivorous Species

Concentrations of organic (OrgHg) and inorganic mercury (InorgHg) were assessed in different fish tissues (liver, muscle, kidney, gut and gonads) and trophic levels collected in an impacted tropical reservoir in southeastern Brazil. Organic mercury concentrations in muscle were remarkably higher in the carnivorous species Hoplias malabaricus and Oligosarcus hepsetus. The ratios of OrgHg in relation to total mercury (%OrgHg) in muscle also varied according to the species trophic level: 93% for carnivores, 84% for omnivores, 73% for algivores/planktivores and 58% for detritivores. The %OrgHg in the gut tissue of carnivores (78%) was much higher than that found in omnivores (30%), possibly reflecting a process of trophic biomagnification in the reservoir. On the other hand, the InorgHg concentrations in muscle decreased with the trophic level increase, suggesting that this form of mercury did not biomagnify through the food web. Gonads contained the least total mercury, and approximately all of this mercury was represented by the organic form (83 to 98%). The kidney and the liver of all fish species contained less than 50% OrgHg. We suggest that the low %OrgHg in the liver is related to different capacities or strategies of OrgHg detoxification by the fish.

scholarly journals Determination of methyl mercury and total mercury in sediment samples collected from canals in HoChiMinh city

2016 ◽  
Vol 19 (4) ◽  
pp. 123-136
Author(s):  
Hien Thai Hoang ◽  
Dong Van Nguyen
Keyword(s):  
Reference Material ◽  
Analytical Method ◽  
Methyl Mercury ◽  
Total Mercury ◽  
Inorganic Mercury ◽  
Absorption Spectrometry ◽  
Internal Reference ◽  
Atomic Fluorescence ◽  
Sediment Samples ◽  
Cold Vapour

In this study, the analysis of methyl mercury (MeHg) and total mercury (T-Hg) was studied using gas chromatographic separation/atomic fluorescence spectrometric detection and cold vapour atomic absorption spectrometry respectively. MeHg was extracted from sediment matrix using HNO3/KCl/CuSO4 into dichloromethane followed ethylation with NaB(C2H5)4 in hexane. Total mercury was digested using three different procedures: EPA 245.1, AOAC 971.21 and our proposed one. The reliability of the analytical method for MeHg was evaluated by the use of the certified reference material ERM CC-580. In addition, the analytical method for total merury was evaluated using a fresh water sediment as an internal reference material, spiked with inorganic mercury, methyl mercury and phenyl mercury. The method detection limits for MeHg and total mercury were 0.08 and 0.15 ng/g (as Hg), respectively. The established analytical methods were applied to analyse MeHg and total mercury in sediment samples collected from canals and rivers in Hochiminh City. The concentrations of methyl mercury and total mercury in sediment samples were 0.08–2.87 ng/g và 14 – 623 ng/g (as Hg, dw). [MeHg]/Σ[Hg] respectirely were in a range of 0.1–2.3 %, which was in good agreement with the published ratios in sediment samples. A good correlation between the concentration of MeHg with total mercury and total organic carbon contents in the studied sediment samples was found.

A Comparison of Mercury Contamination in Mussel and Ammocoete Filter Feeders

2010 ◽  
Vol 1 (2) ◽  
pp. 142-145 ◽  
Author(s):  
Jamie B. Bettaso ◽  
Damon H. Goodman
Keyword(s):  
Methyl Mercury ◽  
Total Mercury ◽  
Mercury Contamination ◽  
Teleost Fishes ◽  
Trinity River ◽  
Longitudinal Gradients ◽  
History Of ◽  
Filter Feeders ◽  
The Trinity ◽  
Mercury Mining

Abstract The Trinity River, California, has an extensive history of gold and mercury mining, and recent studies demonstrated uptake of legacy mercury contamination by teleost fishes. We investigated mercury concentrations of larval lampreys (ammocoetes; Entosphenus spp.) and western pearlshell mussels (Margaritifera falcata) in the Trinity River to determine whether these two long-lived and sedentary filter feeders show site-specific differences in uptake of this contaminant. We analyzed ammocoetes from four sites in the Trinity River and one reference site in the Mad River for total mercury and mussels from three of the Trinity River sites for total and methyl mercury. We identified longitudinal gradients in ammocoete total mercury levels and methyl mercury in mussels. We found a 70% increase in total mercury in ammocoetes between two of the sample sites, suggesting a potential point source of contamination. Ammocoetes contained levels of mercury 12 to 25 times those of mussels from the same site. Our data indicate that ammocoetes may be a preferred organism to sample for mercury contamination and ecological effects compared with mussels in the Trinity River.

Total Mercury, Inorganic Mercury and Methyl Mercury Determination in Red Wine

2011 ◽  
Vol 5 (3) ◽  
pp. 505-511 ◽  
Author(s):  
Valderi Luiz Dressler ◽  
Clarissa Marques Moreira Santos ◽  
Fabiane Goldschmidt Antes ◽  
Fabrina Regia Stum Bentlin ◽  
Dirce Pozebon ◽  
...  
Keyword(s):  
Methyl Mercury ◽  
Total Mercury ◽  
Red Wine ◽  
Inorganic Mercury ◽  
Mercury Determination
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