Regulated versus Constitutive Secretion – A Major Form of Intercellular Communication

2016 ◽  
pp. 376-383
Author(s):  
J. Stöckli ◽  
R.C. Piper ◽  
D.E. James
Keyword(s):  
Intercellular Communication ◽  
Major Form ◽  
Constitutive Secretion

Requirement for Both D Domains of the Propolypeptide in von Willebrand Factor Muitimerization and Storage

1993 ◽  
Vol 70 (06) ◽  
pp. 1053-1057 ◽  
Author(s):  
Agnès M Journet ◽  
Simin Saffaripour ◽  
Denisa D Wagner
Keyword(s):  
Endothelial Cells ◽  
Endoplasmic Reticulum ◽  
Von Willebrand Factor ◽  
Deletion Mutants ◽  
Relative Importance ◽  
D2 Domain ◽  
Constitutive Secretion ◽  
Von Willebrand ◽  
And Storage ◽  
Willebrand Factor

SummaryBiosynthesis of the adhesive glycoprotein von Willebrand factor (vWf) by endothelial cells results in constitutive secretion of small multimers and storage of the largest multimers in rodshaped granules called Weibel-Palade bodies. This pattern is reproduced by expression of pro-vWf in heterologous cells with a regulated pathway of secretion, that store the recombinant protein in similar elongated granules. In these cells, deletion of the vWf prosequence prevents vWf storage. The prosequence, composed of two homologous domains (D1 and D2), actively participates in vWf multimer formation as well. We expressed deletion mutants lacking either the D1 domain (D2vWf) or the D2 domain (D1vWf) in various cell lines to analyze the relative importance of each domain in vWf muitimerization and storage. Both proteins were secreted efficiently without being retained in the endoplasmic reticulum. Despite this, neither multimerized past the dimer stage and they were not stored. We conclude that several segments of the prosequence are jointly involved in vWf muitimerization and storage.

Exosome: An Emerging Source of Biomarkers for Human Diseases

2019 ◽  
Vol 19 (6) ◽  
pp. 387-394 ◽  
Author(s):  
Li Xu ◽  
Long-Fei Wu ◽  
Fei-Yan Deng
Keyword(s):  
Breast Milk ◽  
Intercellular Communication ◽  
Translational Medicine ◽  
Disease Diagnosis ◽  
Human Diseases ◽  
Isolation And Identification ◽  
Biomarker Identification ◽  
Biological Features ◽  
Diagnosis And Prognosis ◽  
Novel Biomarkers

Exosomes are 30-120nm long endocytic membrane-derived vesicles, which are secreted by various types of cells and stably present in body fluids, such as plasma, urine, saliva and breast milk. Exosomes participate in intercellular communication. Recently accumulative studies have suggested that exosomes may serve as novel biomarkers for disease diagnosis and prognosis. Herein, we reviewed the biological features of exosomes, technologies for exosome isolation and identification, as well as progress in exosomal biomarker identification, highlighting the relevance of exosome to human diseases and significance and great potential in translational medicine.

Genetic and Epigenetic Changes of Intercellular Communication Genes During Multistage Carcinogenesis

1999 ◽  
Vol 23 (4) ◽  
pp. 273-279 ◽  
Author(s):  
Hiroshi Yamasaki ◽  
Yasufumi Omori ◽  
Maria-Lucia Zaidan-Dagli ◽  
Nikolai Mironov ◽  
Marc Mesnil ◽  
...  
Keyword(s):  
Intercellular Communication ◽  
Epigenetic Changes ◽  
Multistage Carcinogenesis
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