Thin-Layer Chromatography: Fingerprint Analysis of Plant Materials

2017 ◽  
Author(s):  
Snezana Agatonovic-Kustrin ◽  
David W. Morton
Keyword(s):  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Fingerprint Analysis ◽  
Plant Materials ◽  
Layer Chromatography

scholarly journals Deteksi Adulteran Pada Bahan Baku Sediaan Temulawak (Curcuma xanthorrhiza ROXB) Instan Secara Tlc Fingerprint Analysis

2018 ◽  
Vol 15 (1) ◽  
pp. 38
Author(s):  
Fauzan Zein Muttaqin ◽  
Nurul Aida ◽  
Aiyi Asnawi
Keyword(s):  
Principal Component Analysis ◽  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Curcuma Longa ◽  
Principal Component ◽  
Component Analysis ◽  
Fingerprint Analysis ◽  
Curcuma Xanthorrhiza ◽  
Layer Chromatography

Temulawak (Curcuma xanthorrhiza Roxb) merupakan salah satu jenis tanaman unggulan yang banyak dimanfaatkan masyarakat. Pencampuran adulteran pada bahan baku sediaan temulawak dapat membahayakan kesehatan. Penelitian ini bertujuan untuk mendeteksi adulteran pada bahan baku sediaan temulawak instan. Metode yang digunakan adalah Thin Layer Chromatography (TLC) fingerprint analysis. Sidik jari KLT temulawak dibuat menggunakan rimpang temulawak yang berasal dari Cianjur, Semarang, dan Nusa Tenggara Timur. Sementara sidik jadi kunyit (Curcuma longa)sebagai adulteran utama dibuat menggunakan rimpang kunyi dari Cianjur. Ekstraksi dilakukan dengan metode maserasi menggunakan pelarut etanol 96%. Analisis kromatogram secara kemometrik menggunakan metode Principal Component Analysis (PCA). Nilai loadings Principal Component 1 (PC1) menunjukkan kurva yang linier dan data hasil scores PC1 tersebut dapat membedakan dengan baik sidik jari temulawak dari kunyit dengan nilai scores temulawak dan kunyit berada pada kuadran yang berbeda. Hasil menunjukkan bahwa nilai scores ketiga sampel temulawak instan berada di antara kuadran temulawak dan kunyit (Curcuma Longa L). Dapat disimpulkan bahwa semua sampel positif mengandung adulteran pada temulawak instan.

scholarly journals DIFFERENTIATION OF Curcuma longa, Curcuma xanthorrhiza and Zingiber cassumunar BY THIN LAYER CHROMATOGRAPHY FINGERPRINT ANALYSIS

2011 ◽  
Vol 11 (1) ◽  
pp. 71-74 ◽  
Author(s):  
Mohamad Rafi ◽  
Eti Rohaeti ◽  
Ali Miftahudin ◽  
Latifah K. Darusman
Keyword(s):  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Curcuma Longa ◽  
Chemical Compounds ◽  
Specific Marker ◽  
Fingerprint Analysis ◽  
Fingerprint Pattern ◽  
Curcuma Xanthorrhiza ◽  
Zingiber Cassumunar ◽  
Layer Chromatography

Turmeric (Curcuma longa), java turmeric (Curcuma xanthorrhiza) and cassumunar ginger (Zingiber cassumunar) are widely used in traditional Indonesian medicine. These three herbs have relatively similar rhizomes colour so it is difficult to be differentiated especially if they are in powder form. A rapid and reliable method, thin layer chromatography (TLC) fingerprint, has been developed in order to identify, authenticate and differentiate these three herbs through fingerprint profile of chemical compounds. TLC fingerprints of the three herbs were obtained by visualization of separate zones with visible and UV (254 and 366 nm) light. The TLC fingerprint pattern is different each other and showed a specific marker zones respectively. Therefore, TLC fingerprint can be utilized for identification, authentication and differentiation method in quality control of the three herbs tested.

scholarly journals Assessment of the quality of peppermint leaves by thin-layer chromatography in some objects of plant origin

2020 ◽  
Vol 19 (5-6) ◽  
pp. 148-154
Author(s):  
Vali A. Sahratov ◽  
Tamara L. Malkova ◽  
Ludmila N. Karpova ◽  
Anna A. Pospelova
Keyword(s):  
Medicinal Plant ◽  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Biologically Active ◽  
Mentha Piperita ◽  
Plant Origin ◽  
Plant Materials ◽  
Layer Chromatography ◽  
Active Substances

The State Pharmacopoeia of the XIV edition defines the approach for the assessment of the quality of medicinal plant materials, it deals with the identification of the main groups of biologically active substances by thin layer chromatography. According to this approach, the analysis of some types of medicinal plant materials as a part of some objects of plant origin was carried out. The article presents the quality assessment algorithm as examplified by peppermint leaves (Mentha piperita L.), which are part of a variety of plant object.

scholarly journals Quantitative Determination of Triterpenes from Amphiptherygium adstringens by Liquid Chromatography and Thin-Layer Chromatography and Morphological Analysis of Cuachalalate Preparations

2006 ◽  
Vol 89 (1) ◽  
pp. 1-7 ◽  
Author(s):  
Andrés Navarrete ◽  
Bharathi Avula ◽  
Vaishali C Joshi ◽  
Xiuhong Ji ◽  
Paul Hersh ◽  
...  
Keyword(s):  
Thin Layer ◽  
Thin Layer Chromatography ◽  
High Performance ◽  
Limit Of Detection ◽  
Gastric Ulcers ◽  
Array Detector ◽  
Plant Materials ◽  
Relative Standard ◽  
Layer Chromatography

Abstract Amphiptherygium adstringens (Anacardiaceae/Julianaceae), local name cuachalalate, is used in folk medicine for the treatment of cholelithiasis, fevers, fresh wounds, hypercholesterolemia, gastritis, gastric ulcers, and cancer of the gastrointestinal tract. The development of column high-performance liquid chromatographyphotodiode array detector (LC-PDA) and high-performance thin-layer chromatography (HPTLC)densitometry methods for the determination of masticadienonic acid and 3-hydroxymasticadienonic acid in cuachalalate preparations is described in this paper. Good separation of the compounds could be achieved by both methods. Either might be preparable depending on the requirements. The LC separation was performed on a Phenomenex Synergi MAX-RP 80A reversed-phase column operated at 40C with detection at 215 nm. The plant materials were extracted with methanol by sonication. The triterpenes present in the plant material and commercial extracts were separated with an acetonitrilewater reagent alcohol isocratic system. The limit of detection was 0.10.2 g/mL. The relative standard deviation values for the determination of triterpenes in plant extracts were less than 1.00%. This is the first report of an analytical method developed for the quantitative analysis of triterpenes from Amphiptherygium adstringens by LC-PDA and HPTLC. The stem bark showed higher amounts of triterpenes, and low amounts in root and stem root. The microscopic description of the crude drug of cuachalalate was also provided.

Determination of Linuron and Its Known and/or Suspected Metabolites in Crop Materials

1967 ◽  
Vol 50 (4) ◽  
pp. 911-917
Author(s):  
Stanley E Katz
Keyword(s):  
Thin Layer ◽  
Acid Hydrolysis ◽  
Thin Layer Chromatography ◽  
Aqueous Phase ◽  
Alkaline Hydrolysis ◽  
Ammonium Hydroxide ◽  
Enzymatic Digestion ◽  
Plant Materials ◽  
Layer Chromatography

Abstract Linuron and its known and/or suspected metabolites are extracted from crops with acetone. The acetone is evaporated, and the aqueous residue containing precipitated plant materials and linuron and metabolites is extracted several times with hexane to remove linuron and any 3,4-dichloroaniline, a metabolite. The 3,4-dichloroaniline is separated from the linuron by HC1 extraction. Other possible metabolites, 3-(3,4- dichlorophenyl)-l-methoxy urea, 3-(3,4-dichlorophenyl)- l-methyl urea, and 3-(3,4-dichlorophenyl) urea, remain in the aqueous phase which is made basic with ammonium hydroxide. These metabolites are extracted into hexane and identified by thin layer chromatography. Linuron and metabolites other than 3,4-dichloroaniline can be determined colorimetrically after acid hydrolysis to the aniline, followed by a diazotization reaction and coupling with iV-(l-naphthyl) ethylenediamine. Levels as low as 0.02 ppm linuron can be detected. Recoveries of 98.9% were found. Bound linuron can be determined by alkaline hydrolysis or by enzymatic digestion of crop material which frees the undegraded bound linuron

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