scholarly journals Deteksi Adulteran Pada Bahan Baku Sediaan Temulawak (Curcuma xanthorrhiza ROXB) Instan Secara Tlc Fingerprint Analysis

2018 ◽  
Vol 15 (1) ◽  
pp. 38
Author(s):  
Fauzan Zein Muttaqin ◽  
Nurul Aida ◽  
Aiyi Asnawi
Keyword(s):  
Principal Component Analysis ◽  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Curcuma Longa ◽  
Principal Component ◽  
Component Analysis ◽  
Fingerprint Analysis ◽  
Curcuma Xanthorrhiza ◽  
Layer Chromatography

Temulawak (Curcuma xanthorrhiza Roxb) merupakan salah satu jenis tanaman unggulan yang banyak dimanfaatkan masyarakat. Pencampuran adulteran pada bahan baku sediaan temulawak dapat membahayakan kesehatan. Penelitian ini bertujuan untuk mendeteksi adulteran pada bahan baku sediaan temulawak instan. Metode yang digunakan adalah Thin Layer Chromatography (TLC) fingerprint analysis. Sidik jari KLT temulawak dibuat menggunakan rimpang temulawak yang berasal dari Cianjur, Semarang, dan Nusa Tenggara Timur. Sementara sidik jadi kunyit (Curcuma longa)sebagai adulteran utama dibuat menggunakan rimpang kunyi dari Cianjur. Ekstraksi dilakukan dengan metode maserasi menggunakan pelarut etanol 96%. Analisis kromatogram secara kemometrik menggunakan metode Principal Component Analysis (PCA). Nilai loadings Principal Component 1 (PC1) menunjukkan kurva yang linier dan data hasil scores PC1 tersebut dapat membedakan dengan baik sidik jari temulawak dari kunyit dengan nilai scores temulawak dan kunyit berada pada kuadran yang berbeda. Hasil menunjukkan bahwa nilai scores ketiga sampel temulawak instan berada di antara kuadran temulawak dan kunyit (Curcuma Longa L). Dapat disimpulkan bahwa semua sampel positif mengandung adulteran pada temulawak instan.

scholarly journals DIFFERENTIATION OF Curcuma longa, Curcuma xanthorrhiza and Zingiber cassumunar BY THIN LAYER CHROMATOGRAPHY FINGERPRINT ANALYSIS

2011 ◽  
Vol 11 (1) ◽  
pp. 71-74 ◽  
Author(s):  
Mohamad Rafi ◽  
Eti Rohaeti ◽  
Ali Miftahudin ◽  
Latifah K. Darusman
Keyword(s):  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Curcuma Longa ◽  
Chemical Compounds ◽  
Specific Marker ◽  
Fingerprint Analysis ◽  
Fingerprint Pattern ◽  
Curcuma Xanthorrhiza ◽  
Zingiber Cassumunar ◽  
Layer Chromatography

Turmeric (Curcuma longa), java turmeric (Curcuma xanthorrhiza) and cassumunar ginger (Zingiber cassumunar) are widely used in traditional Indonesian medicine. These three herbs have relatively similar rhizomes colour so it is difficult to be differentiated especially if they are in powder form. A rapid and reliable method, thin layer chromatography (TLC) fingerprint, has been developed in order to identify, authenticate and differentiate these three herbs through fingerprint profile of chemical compounds. TLC fingerprints of the three herbs were obtained by visualization of separate zones with visible and UV (254 and 366 nm) light. The TLC fingerprint pattern is different each other and showed a specific marker zones respectively. Therefore, TLC fingerprint can be utilized for identification, authentication and differentiation method in quality control of the three herbs tested.

scholarly journals Antimicrobial Potential of Single Metabolites of Curcuma longa Assessed in the Total Extract by Thin-Layer Chromatography-Based Bioautography and Image Analysis

2019 ◽  
Vol 20 (4) ◽  
pp. 898 ◽  
Author(s):  
Lidia Czernicka ◽  
Agnieszka Grzegorczyk ◽  
Zbigniew Marzec ◽  
Beata Antosiewicz ◽  
Anna Malm ◽  
...  
Keyword(s):  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Curcuma Longa ◽  
Antimicrobial Properties ◽  
Solvent System ◽  
Total Extract ◽  
Antimicrobial Potential ◽  
Crude Extracts ◽  
Imagej Software ◽  
Layer Chromatography

Curcuma longa from Zingiberaceae belongs to the major spices consumed around the world, known from its cholagogue, anti-inflammatory, and antimicrobial properties. Lack of data on the activity of single components of turmeric extract encouraged the authors to apply TLC (thin-layer chromatography) based bioautography studies to reveal its antimicrobial constituents and construct a universal platform for the bioactivity assessment of crude extracts, with help of a freeware ImageJ software. This optimized chromatographic bioassay performed on diethyl ether and methanol extracts of Curcuma longa was successfully applied on the total extract and revealed the antimicrobial potential of single components against a variety of Gram-positive strains, with no need for their isolation from the mixture. The obtained results were further confronted with a classic microdilution antimicrobial assay on the isolates, purified from the crude extracts by centrifugal partition chromatography in the following solvent system: heptane-chloroform-methanol-water (5:6:3:2) (v/v/v/v).

scholarly journals The Authentication of Java Turmeric (Curcuma xanthorrhiza) Using Thin Layer Chromatography and 1H-NMR Based-Metabolite Fingerprinting Coupled with Multivariate Analysis

Molecules ◽  
2020 ◽  
Vol 25 (17) ◽  
pp. 3928
Author(s):  
Abdul Rohman ◽  
Theresia Wijayanti ◽  
Anjar Windarsih ◽  
Sugeng Riyanto
Keyword(s):  
Multivariate Analysis ◽  
Thin Layer ◽  
Thin Layer Chromatography ◽  
1H Nmr ◽  
Proton Nuclear Magnetic Resonance ◽  
Orthogonal Projections ◽  
Data Set ◽  
Metabolite Fingerprinting ◽  
Curcuma Xanthorrhiza ◽  
Layer Chromatography

The identification of adulteration practices of medicinal plants used as herbal medicine is very important to ensure the quality, safety, and efficacy. In this study, thin layer chromatography (TLC) and proton nuclear magnetic resonance (1H-NMR)-based metabolite fingerprinting coupled with multivariate analysis were used for authentication of Curcuma xanthorrhiza extract from Curcuma aeruginosa. Curcumin contents obtained from C. xanthorrhiza extract from various regions were in the range of 0.74%–1.23%. Meanwhile, curcumin contents obtained from C. xanthorrhiza extract adulterated with 0%, 10%, 25%, 40%, 50%, and 75% of C. aeruginosa were 1.02%, 0.96%, 0.86%, 0.69%, 0.43%, and 0.27%, respectively. The decreasing of curcumin contents in adulterant concentrations of 40% and more in C. xanthorrhiza rhizome could indicate the adulteration with other rhizomes. Multivariate analysis of PCA (principal component analysis) using data set obtained from 1H-NMR spectra clearly discriminated pure and adulterated C. xanthorrhiza with C. aeruginosa. OPLS-DA (orthogonal projections to latent structures-discriminant analysis) successfully classified pure and adulterated C. xanthorrhiza with higher R2X (0.965), R2Y (0.958), and Q2(cum) (0.93). It can be concluded that 1H-NMR-based metabolite fingerprinting coupled with PCA and OPLS-DA offers an adequate method to assess adulteration practice and to evaluate the authentication of C. xanthorrhiza extracts.

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