Enzymatic Methods for Lactic and Pyruvic Acids

Rumen fluid and cellulase based techniques are widely used to predict the digestibility of compound feeds and their ingredients. Recently gammanase enzymes have been added to some cellulase based techniques (Dowman, 1993; De Boever et al., 1994). Few comparisons of these techniques have involved by-product concentrate ingredients. The objective of this experiment was to compare the ability of three techniques, in vitro rumen fluid (RF), pepsin cellulase gammanase (PCG), and neutral detergent cellulase gammanase (NCDG), to predict the in vivo organic matter digestibility (OMD) of concentrate ingredients.

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Human lipoproteins comprise at least 12 different classes that are 1 lognormally distributed.

10.1101/2021.03.19.21253934 ◽

2021 ◽

Author(s):

Tomokazu Konishi ◽

Risako Fujiwara ◽

Tadaaki Saito ◽

Nozomi Satou ◽

...

Keyword(s):

Human Serum ◽

Separation Method ◽

High Gravity ◽

Serum Samples ◽

Rapid Separation ◽

Human Serum Samples ◽

Multiple Factors ◽

Mixed Classes ◽

Time Required ◽

Enzymatic Methods

Lipoproteins in medical samples have been measured by enzymatic methods that coincide with conventional ultracentrifugation. However, the high gravity and time required for ultracentrifugation can cause sample degradation. This study presents the results of HPLC, a gentler and rapid separation method, for 55 human serum samples. The elution patterns were analysed parametrically, and the attribute of each class was confirmed biochemically. Human samples contained 12 classes of lipoproteins, each of which may consist primarily of proteins. There are three classes of VLDLs. The level of each class was distributed lognormally, and the standard amount and the 95% range were estimated. Enzymatic methods measure the levels of several mixed classes. This lognormal character suggests that the levels are controlled by the synergy of multiple factors.

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Enzymatic Methods for Lactic and Pyruvic Acids

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The prediction of digestibility by rumen fluid and enzymatic methods

Human lipoproteins comprise at least 12 different classes that are 1 lognormally distributed.

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