Formulation design considerations and good practice for live attenuated vaccine development

2022 ◽  
pp. 27-78
Author(s):  
Lee C. Smith ◽  
Paul Nelson
Keyword(s):  
Vaccine Development ◽  
Good Practice ◽  
Formulation Design ◽  
Live Attenuated Vaccine ◽  
Design Considerations ◽  
Attenuated Vaccine

Livening up pertussis vaccine development

2020 ◽  
Vol 12 (529) ◽  
pp. eaba9014
Author(s):  
Michelle Boyle
Keyword(s):  
Pertussis Vaccine ◽  
Bordetella Pertussis ◽  
Vaccine Development ◽  
Live Attenuated Vaccine ◽  
Attenuated Vaccine ◽  
Functional Antibodies

Vaccination with a Bordetella pertussis live attenuated vaccine induces a beneficial TH1 cell bias and broadly targeting functional antibodies in humans.

scholarly journals A Review on Dengue Vaccine Development

Vaccines ◽  
2020 ◽  
Vol 8 (1) ◽  
pp. 63 ◽  
Author(s):  
Sheng-Qun Deng ◽  
Xian Yang ◽  
Yong Wei ◽  
Jia-Ting Chen ◽  
Xiao-Jun Wang ◽  
...  
Keyword(s):  
Global Health ◽  
Subunit Vaccine ◽  
Vaccine Development ◽  
Clinical Use ◽  
Risk Of Infection ◽  
Vaccine Recombinant ◽  
Dengue Vaccine ◽  
Live Attenuated Vaccine ◽  
Attenuated Vaccine ◽  
Vectored Vaccine

Dengue virus (DENV) has become a global health threat with about half of the world’s population at risk of infection. Although the disease caused by DENV is self-limiting in the first infection, the antibody-dependent enhancement (ADE) effect increases the mortality in the second infection with a heterotypic virus. Since there is no specific efficient medicine in treatment, it is urgent to develop vaccines to prevent infection and disease progression. Currently, only a live attenuated vaccine, chimeric yellow fever 17D—tetravalent dengue vaccine (CYD-TDV), has been licensed for clinical use in some countries, and many candidate vaccines are still under research and development. This review discusses the progress, strengths, and weaknesses of the five types of vaccines including live attenuated vaccine, inactivated virus vaccine, recombinant subunit vaccine, viral vectored vaccine, and DNA vaccine.

scholarly journals Streptococcus iniae M-Like Protein Contributes to Virulence in Fish and Is a Target for Live Attenuated Vaccine Development

PLoS ONE ◽  
2008 ◽  
Vol 3 (7) ◽  
pp. e2824 ◽  
Author(s):  
Jeffrey B. Locke ◽  
Ramy K. Aziz ◽  
Mike R. Vicknair ◽  
Victor Nizet ◽  
John T. Buchanan
Keyword(s):  
Vaccine Development ◽  
Streptococcus Iniae ◽  
Live Attenuated Vaccine ◽  
Attenuated Vaccine

scholarly journals Expression analysis of centrin gene in promastigote and amastigote forms of leishmania infantum iranian isolates: a promising target for live attenuated vaccine development against canine leishmaniasis

2021 ◽  
Vol 17 (1) ◽  
Author(s):  
Mohammad Javad Abbaszadeh Afshar ◽  
Samira Elikaee ◽  
Reza Saberi ◽  
Sina Mohtasebi ◽  
Mehdi Mohebali
Keyword(s):  
Leishmania Infantum ◽  
Vaccine Development ◽  
Essential Gene ◽  
Canine Leishmaniasis ◽  
Logarithmic Phase ◽  
Growth Phases ◽  
Live Attenuated Vaccine ◽  
Intracellular Amastigotes ◽  
Attenuated Vaccine ◽  
Promising Target

Abstract Background Leishmania parasites express various essential proteins in different growth phases (logarithmic/stationary) and forms (promastigote/amastigote). Targeting the genes encoding such proteins paves the way for controlling these parasites. Centrin is an essential gene, which its protein product seems to be vital for the proliferation of Leishmania parasites. Herein, this study was contrived to analyze the expression level of the centrin gene in different growth phases and forms of Leishmania infantum (L. infantum) parasites isolated from various endemic areas of canine leishmaniasis (CanL) in Iran. Results All three collected isolates were identified as L. infantum using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). Real-time reverse transcription (RT)-PCR revealed a statistically significant up-regulation (3.13-fold) in the logarithmic phase promastigotes compared to stationary ones (p < 0.01), whereas centrin was expressed equally in intracellular amastigotes at different time points during cell culture. Also, our finding revealed a slight up-regulation of the centrin gene (1.22-fold) in the intracellular amastigotes compared to logarithmic phase promastigotes, which was found statistically non-significant (p > 0.05). Conclusions Centrin gene in Iranian isolates of L. infantum is more expressed in exponential than stationary phases and seems to be considered as a promising target in the development of a genetically modified live attenuated vaccine for CanL control.

scholarly journals Immunogenicity and Protective Efficacy of an Intranasal Live-attenuated Vaccine Against SARS-CoV-2

iScience ◽  
2021 ◽  
pp. 102941
Author(s):  
Jun-Guy Park ◽  
Fatai S. Oladunni ◽  
Mohammed A. Rohaim ◽  
Jayde Whittingham-Dowd ◽  
James Tollitt ◽  
...  
Keyword(s):  
Protective Efficacy ◽  
Live Attenuated Vaccine ◽  
Attenuated Vaccine
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