Magnetic cell separation

Abstract Abstract 3718 CD25+Foxp3+ regulatory T cell (Treg) bear great potential to prevent or treat a variety of immune mediated diseases, including autoimmunity, organ rejection or GvHD. Currently Treg for clinical application can be separated by magnetic cell separation via the CliniMACS® Plus Instrument using CD25 enrichment plus/minus prior depletion of CD8 or CD19 positive cells. With this technology Treg can be enriched to a mean purity of about 50% and first clinical trials for prevention of GvHD show no adverse effects at all. Despite these promising results, concerns have been raised whether in the setting of organ transplantation or autoimmunity higher Treg purities and/or the in vitro expansion of Treg without loss of Foxp3+ expression are required. Therefore, we have optimized the parameters for CD25 enrichment via CliniMACS to achieve higher purity of the isolated Treg. The purity of Treg could be increased by about 20–30% resulting in an average purity of 70–80% of Foxp3+ Treg. We have also developed a protocol for the in vitro expansion of CliniMACS isolated Treg using CD3/CD28 coated MACSiBead™Particles. In the presence of Rapamycin CliniMACS isolated Treg could be expanded about 20 fold with a single round of stimulation. Importantly Foxp3+ expression was not affected by the expansion but remained constant at about 70–80%. Similarly the expression of effector cytokines by expanded Treg was greatly suppressed by Rapamycin. These data show that Treg for clinical application can efficiently be isolated with high purity via CliniMACS and subsequently be expanded in vitro without loss of Foxp3 expression. Disclosures: Conrads: Miltenyi Biotec: Employment. Schmitz:Miltenyi Biotec: Employment. Assenmacher:m: Employment. Niemand:Miltenyi Biotec: Employment. Scheffold:Miltenyi Biotec: Employment.

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Dynamics of magnetic particles in cylindrical Halbach array: implications for magnetic cell separation and drug targeting

Medical & Biological Engineering & Computing ◽

10.1007/s11517-010-0636-8 ◽

2010 ◽

Vol 48 (8) ◽

pp. 745-753 ◽

Cited By ~ 23

Author(s):

Peter Babinec ◽

Andrej Krafčík ◽

Melánia Babincová ◽

Joseph Rosenecker

Keyword(s):

Cell Separation ◽

Drug Targeting ◽

Magnetic Particles ◽

Halbach Array ◽

Magnetic Cell Separation

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Microdevice for magnetic cell separation simple fabrication and simulation analysis

2017 IEEE International Conference on Power, Control, Signals and Instrumentation Engineering (ICPCSI) ◽

10.1109/icpcsi.2017.8392066 ◽

2017 ◽

Author(s):

O. Saeed ◽

Yulin Deng

Keyword(s):

Cell Separation ◽

Simulation Analysis ◽

Magnetic Cell Separation

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Functionalized Magnetic Nanoparticles for the Detection and Quantitative Analysis of Cell Surface Antigen

BioMed Research International ◽

10.1155/2013/349408 ◽

2013 ◽

Vol 2013 ◽

pp. 1-9 ◽

Cited By ~ 14

Author(s):

Daryoush Shahbazi-Gahrouei ◽

Mohammad Abdolahi ◽

Sayyed Hamid Zarkesh-Esfahani ◽

Sophie Laurent ◽

Corine Sermeus ◽

...

Keyword(s):

Magnetic Nanoparticles ◽

Cell Surface ◽

Surface Antigen ◽

Cell Separation ◽

Cell Surface Antigen ◽

Cell Surface Antigens ◽

Vitro Assay ◽

Gold Standard Method ◽

Magnetic Cell Separation

Cell surface antigens as biomarkers offer tremendous potential for early diagnosis, prognosis, and therapeutic response in a variety of diseases such as cancers. In this research, a simple, rapid, accurate, inexpensive, and easily available in vitro assay based on magnetic nanoparticles and magnetic cell separation principle was applied to identify and quantitatively analyze the cell surface antigen expression in the case of prostate cancer cells. Comparing the capability of the assay with flow cytometry as a gold standard method showed similar results. The results showed that the antigen-specific magnetic cell separation with antibody-coated magnetic nanoparticles has high potential for quantitative cell surface antigen detection and analysis.

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