scholarly journals Neonatal Calf Diarrhea Virus

Author(s):  
C.A. MEBUS
1971 ◽  
Vol 8 (5-6) ◽  
pp. 490-505 ◽  
Author(s):  
C. A. Mebus ◽  
E. L. Stair ◽  
N. R. Underdahl ◽  
M. J. Twiehaus

Gross, immunofluorescent, and light microscopic findings in seven gnotobiotic calves inoculated orally with a Reo-like neonatal calf diarrhea virus were compared to findings in three control gnotobiotic calves. Neonatal calf diarrhea virus infected primarily the villous epithelium of the small intestine. Calves examined within 1.5 h after onset of diarrhea had tall columnar immunofluorescent villous epithelial cells in the middle and lower small intestine. Calves examined 2–4.5 h after onset of diarrhea had cuboidal to squamous villous epithelial cells and an increase in reticulum-like cells in the villous lamina propria of the middle and lower small intestine. Viral tilers were 106 and 108 in colonic contents from two calves inoculated with cell-culture-adapted virus and necropsied, respectively, 2 and 6 h after onset of diarrhea.


1995 ◽  
Vol 33 (4) ◽  
pp. 990-994 ◽  
Author(s):  
W Lu ◽  
G E Duhamel ◽  
Y Hoshino ◽  
D A Benfield ◽  
E A Nelson ◽  
...  

1976 ◽  
Vol 3 (3) ◽  
pp. 376-377
Author(s):  
M W Peterson ◽  
R S Spendlove ◽  
R A Smart

Thirty-four calf and five infant fecal specimens were tested for the neonatal calf diarrhea virus (NCDV) and for the reovirus-like infantile diarrhea agent; respectively. The procedures used were the fluorescent virus precipitin test and immune electron microscopy. Fourteen of the calf stools contained detectable NCDV, and four of the five infant stools contained the reovirus-like human agent. Infectious NCDV was detected in four of the 34 calf fecal specimens when Madin-Darby bovine kidney cell cultures that had been inoculated with supernatant fluids from stool suspensions were stained with fluorescent antibody. The 20 calf stools that did not have detectable virus were examined for the bovine corona diarrhea virus. Coronavirus was found in two of these specimens.


1977 ◽  
Vol 5 (1) ◽  
pp. 1-4
Author(s):  
S Matsuno ◽  
S Inouye ◽  
R Kono

Neonatal calf diarrhea virus (a bovine rotavirus) formed distinct plaques in monolayers of MA-104 cells, an established macacus rhesus monkey kidney cell line, when diethylaminoethyl dextran and trypsin were included in the overlay medium. By using this plaque assay method, titration of neutralizing antibody to neonatal calf diarrhea virus was made feasible. It was demonstrated that some human sera contained neutralizing antibody to this agent.


2016 ◽  
Vol 99 (8) ◽  
pp. 6563-6571 ◽  
Author(s):  
Stephani Fischer ◽  
Rolf Bauerfeind ◽  
Claus-Peter Czerny ◽  
Stephan Neumann

2020 ◽  
Vol 16 (1) ◽  
Author(s):  
Celina Guadalupe Vega ◽  
Marina Bok ◽  
Maren Ebinger ◽  
Lucía Alejandra Rocha ◽  
Alejandra Antonella Rivolta ◽  
...  

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