scholarly journals Strategies for Characterization of Low-Abundant Intact or Truncated Low-Molecular-Weight Proteins From Human Plasma

2017 ◽  
pp. 105-123 ◽  
Author(s):  
Tanxi Cai ◽  
Fuquan Yang
Keyword(s):  
Molecular Weight ◽  
Human Plasma ◽  
Low Molecular Weight ◽  
Low Molecular Weight Proteins

Isolation and characterization of a low molecular weight growth-promoting factor from human plasma

Life Sciences ◽  
1987 ◽  
Vol 41 (3) ◽  
pp. 297-304 ◽  
Author(s):  
M.H. Heulin ◽  
J. Rajoelina ◽  
M. Artur ◽  
C. Geschier ◽  
J. Straczek ◽  
...  
Keyword(s):  
Molecular Weight ◽  
Human Plasma ◽  
Low Molecular Weight ◽  
Weight Growth ◽  
Isolation And Characterization ◽  
Growth Promoting

The Characterization of Two New Low Molecular Weight Proteins (LMPs) from Streptococcus pyogenes

1992 ◽  
Vol 277 (1) ◽  
pp. 1-9 ◽  
Author(s):  
Dieter Gerlach ◽  
Heide Alouf ◽  
Ladislav Morávek ◽  
Manfred Pavlik ◽  
Werner Köhler
Keyword(s):  
Molecular Weight ◽  
Streptococcus Pyogenes ◽  
Low Molecular Weight ◽  
Low Molecular Weight Proteins

Mass spectrometric characterization of membrane integral low molecular weight proteins from photosystem II in barley etioplasts

PROTEOMICS ◽  
2009 ◽  
Vol 9 (3) ◽  
pp. 625-635 ◽  
Author(s):  
Matthias Plöscher ◽  
Bernhard Granvogl ◽  
Mikael Zoryan ◽  
Veronika Reisinger ◽  
Lutz Andreas Eichacker
Keyword(s):  
Molecular Weight ◽  
Photosystem Ii ◽  
Mass Spectrometric ◽  
Low Molecular Weight ◽  
Low Molecular Weight Proteins

scholarly journals Use of Tri-Chloroacetic Acid /Acetone/polyethylene glycol for protein purification from HIV-1 infected human plasma

2019 ◽  
Author(s):  
Sushanta Kumar Barik ◽  
Keshar Kunja Mohanty ◽  
Deepa Bisht ◽  
Partha Sarathi Mohanty ◽  
Shripad Patil ◽  
...  
Keyword(s):  
Molecular Weight ◽  
Polyethylene Glycol ◽  
Protein Purification ◽  
Human Plasma ◽  
Water Soluble ◽  
Low Molecular Weight ◽  
High Concentration ◽  
Low Concentration ◽  
Low Molecular Weight Proteins ◽  
Hiv 1

Abstract Human plasma contains high amount of abundant proteins like albumin and globulin. Normally, the proteins having potential for biomarkers are present in very low concentration in human plasma. To resolve the low concentration proteins in polyacrylamide gel, the removal of high abundant proteins from plasma are very essential. Polyethylene glycol is a nontoxic, water soluble synthetic polymer has several applications in chemical and biomedical industries. Various molecular variants of poly ethylene glycol is available and used in protein purification. The mechanism behind the use of high concentration of polyethylene glycol is it binds the molecule in more compact or interpenetrates forming a gel like network surrounding the molecule. Polyethylene glycol -6000 removes the high abundant proteins like Albumin and Globulin in the HIV -1 infected plasma samples and concentrates the low molecular weight proteins as the low molecular weight proteins are essential in biomarker study.

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