Sequential Precipitation and Delipidation Enables Efficient Enrichment of Low-Molecular Weight Proteins and Peptides from Human Plasma

2020 ◽  
Vol 19 (8) ◽  
pp. 3340-3351 ◽  
Author(s):  
Na Li ◽  
Yue Zhou ◽  
Jifeng Wang ◽  
Lili Niu ◽  
Qing Zhang ◽  
...  
Keyword(s):  
Molecular Weight ◽  
Human Plasma ◽  
Low Molecular Weight ◽  
Sequential Precipitation ◽  
Low Molecular Weight Proteins ◽  
Proteins And Peptides

scholarly journals Novel Synthesis of Core-Shell Silica Nanoparticles for the Capture of Low Molecular Weight Proteins and Peptides

Molecules ◽  
2017 ◽  
Vol 22 (10) ◽  
pp. 1712 ◽  
Author(s):  
Sergio Hernandez-Leon ◽  
Jose Sarabia-Sainz ◽  
Gabriela Montfort ◽  
Ana Guzman-Partida ◽  
Maria Robles-Burgueño ◽  
...  
Keyword(s):  
Molecular Weight ◽  
Silica Nanoparticles ◽  
Core Shell ◽  
Low Molecular Weight ◽  
Novel Synthesis ◽  
Low Molecular Weight Proteins ◽  
Proteins And Peptides

scholarly journals Use of Tri-Chloroacetic Acid /Acetone/polyethylene glycol for protein purification from HIV-1 infected human plasma

2019 ◽  
Author(s):  
Sushanta Kumar Barik ◽  
Keshar Kunja Mohanty ◽  
Deepa Bisht ◽  
Partha Sarathi Mohanty ◽  
Shripad Patil ◽  
...  
Keyword(s):  
Molecular Weight ◽  
Polyethylene Glycol ◽  
Protein Purification ◽  
Human Plasma ◽  
Water Soluble ◽  
Low Molecular Weight ◽  
High Concentration ◽  
Low Concentration ◽  
Low Molecular Weight Proteins ◽  
Hiv 1

Abstract Human plasma contains high amount of abundant proteins like albumin and globulin. Normally, the proteins having potential for biomarkers are present in very low concentration in human plasma. To resolve the low concentration proteins in polyacrylamide gel, the removal of high abundant proteins from plasma are very essential. Polyethylene glycol is a nontoxic, water soluble synthetic polymer has several applications in chemical and biomedical industries. Various molecular variants of poly ethylene glycol is available and used in protein purification. The mechanism behind the use of high concentration of polyethylene glycol is it binds the molecule in more compact or interpenetrates forming a gel like network surrounding the molecule. Polyethylene glycol -6000 removes the high abundant proteins like Albumin and Globulin in the HIV -1 infected plasma samples and concentrates the low molecular weight proteins as the low molecular weight proteins are essential in biomarker study.

Simplification of the purification of heat stable recombinant low molecular weight proteins and peptides from GST-fusion products

2021 ◽  
pp. 122627
Author(s):  
Beatrice Sakhel ◽  
Srinivas Jayanthi ◽  
Djamali Muhoza ◽  
Patience Okoto ◽  
Thallapuranam Krishnaswamy Suresh Kumar ◽  
...  
Keyword(s):  
Molecular Weight ◽  
Low Molecular Weight ◽  
Heat Stable ◽  
Fusion Products ◽  
Low Molecular Weight Proteins ◽  
Proteins And Peptides
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