A simple hybridoma screening method for high-affinity monoclonal antibodies using the signal ratio obtained from time-resolved fluorescence assay

2006 ◽  
Vol 351 (2) ◽  
pp. 219-228 ◽  
Author(s):  
Kenji Daigo ◽  
Sui Sugita ◽  
Yasuhiro Mochizuki ◽  
Hiroko Iwanari ◽  
Kanae Hiraishi ◽  
...  
Keyword(s):  
Monoclonal Antibodies ◽  
Screening Method ◽  
Fluorescence Assay ◽  
Time Resolved Fluorescence ◽  
Signal Ratio ◽  
Time Resolved ◽  
High Affinity

A simple time-resolved fluorescence assay for quantitative determination of DOTA chelator

2019 ◽  
Vol 584 ◽  
pp. 113384 ◽  
Author(s):  
Michał Szkop ◽  
Kamil Brygoła ◽  
Magdalena Janczewska ◽  
Tomasz Ciach
Keyword(s):  
Quantitative Determination ◽  
Fluorescence Assay ◽  
Time Resolved Fluorescence ◽  
Time Resolved

Measurement of estrone-3-glucuronide in urine by rapid, homogeneous time-resolved fluoroimmunoassay.

1989 ◽  
Vol 35 (4) ◽  
pp. 555-559 ◽  
Author(s):  
G Barnard ◽  
F Kohen ◽  
H Mikola ◽  
T Lövgren
Keyword(s):  
Monoclonal Antibodies ◽  
Liquid Phase ◽  
Clinical Utility ◽  
Ovarian Function ◽  
Fluorescence Immunoassay ◽  
Time Resolved Fluorescence ◽  
Time Resolved ◽  
Europium Chelate ◽  
Covalently Linked ◽  
Sensitivity Specificity

Abstract We describe a liquid-phase nonseparation time-resolved fluorescence immunoassay for measuring estrone-3-glucuronide in undiluted urine. The sensitivity, specificity, and accuracy are similar to those for a conventional separation fluoroimmunoassay or radioimmunoassay, but the speed, convenience, precision, reliability, and clinical utility of the new method are more advantageous. The labeled antigen, a fluorescent europium chelate covalently linked to estrone-3-glucuronide, is incubated for 10 min with a limited concentration of polyclonal or monoclonal antibodies to estrone-3-glucuronyl-6-bovine serum albumin and 10 microL of standard or sample (undiluted urine) in microtiter wells. The fluorescence emanating from the antibody-free label, which is proportional to the concentration of estrone-3-glucuronide in the standard or sample, is then measured in a time-resolved fluorometer. The method is useful for monitoring ovarian function in women.

scholarly journals Optimization of time-resolved fluorescence assay for detection of europium–tetraazacyclododecyltetraacetic acid-labeled ligand–receptor interactions

2010 ◽  
Vol 398 (1) ◽  
pp. 15-23 ◽  
Author(s):  
Channa R. De Silva ◽  
Josef Vagner ◽  
Ronald Lynch ◽  
Robert J. Gillies ◽  
Victor J. Hruby
Keyword(s):  
Fluorescence Assay ◽  
Time Resolved Fluorescence ◽  
Time Resolved ◽  
Receptor Interactions

A Homogeneous Time-Resolved Fluorescence Assay to Identify Inhibitors of HIV-1 Fusion

2013 ◽  
pp. 11-17
Author(s):  
Liesbet Smeulders ◽  
Lieve Bunkens ◽  
Inge Vereycken ◽  
Koen Van Acker ◽  
Pascale Holemans ◽  
...  
Keyword(s):  
Fluorescence Assay ◽  
Time Resolved Fluorescence ◽  
Time Resolved ◽  
Hiv 1
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