Quantitation of soluble aggregates in recombinant monoclonal antibody cell culture by pH-gradient protein A chromatography

2009 ◽  
Vol 388 (2) ◽  
pp. 273-278 ◽  
Author(s):  
Hai Pan ◽  
Ken Chen ◽  
Matt Pulisic ◽  
Izydor Apostol ◽  
Gang Huang
Keyword(s):  
Cell Culture ◽  
Monoclonal Antibody ◽  
Protein A ◽  
Ph Gradient ◽  
Recombinant Monoclonal Antibody ◽  
Soluble Aggregates

When Good Intentions Go Awry: Modification of a Recombinant Monoclonal Antibody in Chemically Defined Cell Culture by Xylosone, an Oxidative Product of Ascorbic Acid

2015 ◽  
Vol 87 (15) ◽  
pp. 7529-7534 ◽  
Author(s):  
Chris Chumsae ◽  
Patrick Hossler ◽  
Haly Raharimampionona ◽  
Yu Zhou ◽  
Sean McDermott ◽  
...  
Keyword(s):  
Cell Culture ◽  
Monoclonal Antibody ◽  
Ascorbic Acid ◽  
Recombinant Monoclonal Antibody

Effect of methionine oxidation of a recombinant monoclonal antibody on the binding affinity to protein A and protein G

2008 ◽  
Vol 870 (1) ◽  
pp. 55-62 ◽  
Author(s):  
Georgeen Gaza-Bulseco ◽  
Sagar Faldu ◽  
Karen Hurkmans ◽  
Chris Chumsae ◽  
Hongcheng Liu
Keyword(s):  
Monoclonal Antibody ◽  
Binding Affinity ◽  
Protein A ◽  
Protein G ◽  
Methionine Oxidation ◽  
Recombinant Monoclonal Antibody

Principle of Express Selection of Leading Producing Clones of Monoclonal Antibodies in the Development of Stable CHO-Based Cell Lines

2019 ◽  
Vol 35 (4) ◽  
pp. 65-72
Author(s):  
V.I. Pavelko ◽  
I.A. Kirik ◽  
V.N. Bade ◽  
T.O. Malygina ◽  
R.A. Khamitov ◽  
...  
Keyword(s):  
Cell Culture ◽  
Monoclonal Antibody ◽  
Monoclonal Antibodies ◽  
Parallel Processing ◽  
Cell Lines ◽  
Cho Cells ◽  
Fed Batch ◽  
Cho Cell ◽  
Recombinant Monoclonal Antibody ◽  
Selection Of

Growth and productive characteristics of monoclonal cell lines based on CHO cells and producing a therapeutic protein have been monitored using the robot Ambr Tap Biosystems, which permitted to identify the leading line. Twenty four clones producing a recombinant monoclonal antibody were studied under the close to industrial conditions in a fed-batch culturing mode. The ambr®15 cell culture workstation controls 24 disposable mini bioreactors, and offers parallel processing and evaluation of multiple (24) experiments in an automated bench-top system. The volumetric productivity of 24 clones determined by ELISA was 120-450 mg /L. A protocol was shown to select a leader among producing clones for further research. producing clones, mini bioreactor; Ambr Tap Biosystems, fed-batch, monoclonal antibodies, CHO cell culture

Impact of cell culture on recombinant monoclonal antibody product heterogeneity

2016 ◽  
Vol 32 (5) ◽  
pp. 1103-1112 ◽  
Author(s):  
Hongcheng Liu ◽  
Christine Nowak ◽  
Mei Shao ◽  
Gomathinayagam Ponniah ◽  
Alyssa Neill
Keyword(s):  
Cell Culture ◽  
Monoclonal Antibody ◽  
Recombinant Monoclonal Antibody ◽  
Product Heterogeneity ◽  
Monoclonal Antibody Product

Controlling trisulfide modification in recombinant monoclonal antibody produced in fed-batch cell culture

2012 ◽  
Vol 109 (10) ◽  
pp. 2523-2532 ◽  
Author(s):  
Rashmi Kshirsagar ◽  
Kyle McElearney ◽  
Alan Gilbert ◽  
Marty Sinacore ◽  
Thomas Ryll
Keyword(s):  
Cell Culture ◽  
Monoclonal Antibody ◽  
Fed Batch ◽  
Recombinant Monoclonal Antibody ◽  
Batch Cell

scholarly journals Staphylococcus aureus Decolonization of Mice With Monoclonal Antibody Neutralizing Protein A

2018 ◽  
Vol 219 (6) ◽  
pp. 884-888 ◽  
Author(s):  
Xinhai Chen ◽  
Yan Sun ◽  
Dominique Missiakas ◽  
Olaf Schneewind
Keyword(s):  
Staphylococcus Aureus ◽  
Monoclonal Antibody ◽  
Protein A ◽  
Immunoglobulin A ◽  
Systemic Administration ◽  
Staphylococcal Protein A ◽  
Recombinant Monoclonal Antibody ◽  
Invasive Infections ◽  
Selection For ◽  
Hospital Acquired

Abstract Staphylococcus aureus persistently colonizes the nasopharynx of about one-third of the human population, a key risk factor for community- and hospital-acquired invasive infections. Current strategies for S. aureus decolonization include topical and systemic administration of antibiotics, which is associated with selection for antibiotic resistance and posttreatment recolonization. Using a mouse model for S. aureus colonization, we show here that systemic administration of a recombinant monoclonal antibody neutralizing staphylococcal protein A (SpA) can stimulate antibacterial immunoglobulin G and immunoglobulin A responses and promote S. aureus decolonization. These results suggest that antibody neutralizing SpA, a B-cell superantigen, may also be useful for S. aureus decolonization in humans.

Solid-phase immunoelectron microscopy for rapid diagnosis of enteroviruses

1984 ◽  
Vol 42 ◽  
pp. 226-227 ◽  
Author(s):  
K. Pegg-Feige ◽  
F. W. Doane
Keyword(s):  
Electron Microscopy ◽  
Cell Culture ◽  
Immunoelectron Microscopy ◽  
Detection Method ◽  
Solid Phase ◽  
Protein A ◽  
Plant Viruses ◽  
Rapid Diagnosis ◽  
Virus Diagnosis ◽  
Antiviral Antibody

Immunoelectron microscopy (IEM) applied to rapid virus diagnosis offers a more sensitive detection method than direct electron microscopy (DEM), and can also be used to serotype viruses. One of several IEM techniques is that introduced by Derrick in 1972, in which antiviral antibody is attached to the support film of an EM specimen grid. Originally developed for plant viruses, it has recently been applied to several animal viruses, especially rotaviruses. We have investigated the use of this solid phase IEM technique (SPIEM) in detecting and identifying enteroviruses (in the form of crude cell culture isolates), and have compared it with a modified “SPIEM-SPA” method in which grids are coated with protein A from Staphylococcus aureus prior to exposure to antiserum.

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