Selection of appropriate reference genes for gene expression studies by quantitative real-time polymerase chain reaction in cucumber

2010 ◽  
Vol 399 (2) ◽  
pp. 257-261 ◽  
Author(s):  
Hongjian Wan ◽  
Zhenguo Zhao ◽  
Chuntao Qian ◽  
Yihu Sui ◽  
Ahmed Abbas Malik ◽  
...  
Keyword(s):  
Gene Expression ◽  
Polymerase Chain Reaction ◽  
Real Time ◽  
Reference Genes ◽  
Chain Reaction ◽  
Expression Studies ◽  
Polymerase Chain ◽  
Gene Expression Studies ◽  
Selection Of

scholarly journals Validation of the Reference Genes for the Gene Expression Studies in Different Cell Lines of Pig

2021 ◽  
Vol 2021 ◽  
pp. 1-9
Author(s):  
Chun-Di Xie ◽  
Bingyuan Wang ◽  
Zhao-Ji Shen ◽  
Wen-Ye Yao ◽  
Hong Ao ◽  
...  
Keyword(s):  
Gene Expression ◽  
Polymerase Chain Reaction ◽  
Cell Lines ◽  
Reference Genes ◽  
Theoretical Basis ◽  
Target Genes ◽  
Chain Reaction ◽  
Expression Studies ◽  
Polymerase Chain ◽  
Gene Expression Studies

Reverse transcription quantitative real-time polymerase chain reaction is one of the important methods to investigate gene expression in cells and tissues. However, if the data cannot be normalized with appropriate reference genes, the results may be unreliable. In this study, we detected the expression of 15 reference genes in three pig cell lines. The results showed that SDHA and ALDOA were the most stable reference genes in 3D4/21 cells. TOP2B, TBP, and PPIA were the most stable reference genes in PK-15 cells. SDHA and ALDOA were the most stable reference genes in IPEC-J2 cells. In addition, each cell line only needs to use two reference genes to standardize the expression of target genes. Taken together, this study provides a reference for different pig cell lines to select reference genes and also provides a theoretical basis for the use of these cell lines in related functional researches.

Comparison of reference genes for quantitative real-time polymerase chain reaction analysis of gene expression in sugarcane

2004 ◽  
Vol 22 (4) ◽  
pp. 325-337 ◽  
Author(s):  
Hayati M. Iskandar ◽  
Robert S. Simpson ◽  
Rosanne E. Casu ◽  
Graham D. Bonnett ◽  
Donald J. Maclean ◽  
...  
Keyword(s):  
Gene Expression ◽  
Polymerase Chain Reaction ◽  
Real Time ◽  
Reference Genes ◽  
Polymerase Chain Reaction Analysis ◽  
Chain Reaction ◽  
Polymerase Chain
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